scholarly journals Genetic Variation of Clupeonella engrauliformis Populations Inferred from RFLP Analysis of Mitochondrial DNA D-loop Region on the Southern Coast of the Caspian Sea, Iran

2010 ◽  
Vol 22 (3) ◽  
Author(s):  
F. LALOEI
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Caspian Sea ◽  
Rflp Analysis ◽  
Southern Coast ◽  
The Caspian Sea ◽  
Loop Region ◽  
D Loop

PCR–RFLP analysis of mitochondrial DNA for identification of Rutilus rutilus caspicus populations on the southern coast of the Caspian Sea, Iran

2006 ◽  
Vol 86 (6) ◽  
pp. 1463-1467 ◽  
Author(s):  
Sohrab Rezvani ◽  
Amin Eimanifar ◽  
Reza Aghili ◽  
Faramarz Laloei
Keyword(s):  
Caspian Sea ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Rutilus Rutilus ◽  
Southern Coast ◽  
The Caspian Sea ◽  
Length Polymorphisms ◽  
Pcr Rflp ◽  
Nucleotide Divergence ◽  
Genetic Pools

Genetic analysis using restriction fragment length polymorphisms (RFLPs) of cytochrome b in mtDNA was made to clarify genetic variations among two Iranian Rutilus rutilus caspicus populations of commercial importance from the southern coast of the Caspian Sea. Polymorphism was detected using six restriction enzymes and a total of six composite haplotypes were identified. Four haplotypes were rare occurring only once in two regions (west and east of the southern Caspian Sea). Nucleotide and haplotype diversities were higher in the south-west region of the Caspian Sea (π=3.43%, h=23.3).The nucleotide divergence between the two populations was low (0.064%). The test for heterogeneity of composite haplotype frequencies gave no significant outcome for all samples (χ2=0.137, P≤0.05). The results indicate that significant attention should be paid to the genetic characterization of R. rutilus caspicus populations for conservation of their genetic pools and aquaculture policies at the coastlines of the Caspian Sea.

scholarly journals Mitochondrial Diversity of the East Balkan Swine (Sus scrofa f. domestica) in South-Eastern Bulgaria

2019 ◽  
Vol 69 (2) ◽  
pp. 229-236
Author(s):  
Nadezhda Palova ◽  
Iskra Yankova ◽  
Boyko Neov ◽  
Peter Hristov ◽  
Georgi Radoslavov
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Sus Scrofa ◽  
Genetic Profile ◽  
Scientific Center ◽  
Equal Distribution ◽  
Loop Region ◽  
D Loop ◽  
Purposeful Selection ◽  
Balkan Mountains

Abstract The East Balkan Swine (EBS) is the only preserved local swine breed in Bulgaria and one of the few indigenous pig breeds in Europe. The EBS is distributed in the region of Eastern Balkan Mountains and the Strandja Mountain. To reveal the breed’s genetic profile, we analyzed 50 purebred individuals according to mitochondrial DNA (D-loop region, HVR1) and sequence analysis in the Scientific Center of Agriculture (Sredets region) in the country. The obtained results show the presence of four haplotypes: three Asian specific haplotypes (H1, H2, and H3) and the European specific E1a1. The haplotypes H2 (6 %) and H3 (2 %) were newly described and were branched from the basic clade H1 (90 %). All haplotypes belong to the Asiatic clade A (98 %), except one sample assigned to the European haplogroup E1 (2 %) in contrast to samples from East North Bulgaria where Asiatic and Europen clades were with almost equal distribution. The coexistence of two mtDNA clades in EBS in Bulgaria may be related to the source of the pig populations and/or the historical crossbreeding with imported pigs. In conclusion, due to its native origin, the East Balkan Swine may be the only possible option for a solution to the exhaustion of the beneficial genetic variation of available cultural breeds. With its participation, high-productive populations can be restored and established after a long and purposeful selection.

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