Genetic Variation in Yellowfin Tuna Thunnus Albacares (Bonnaterre, 1788) Along Indian Coast Using Pcr-Rflp Analysis of Mitochondrial Dna D-Loop Region

2012 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Swaraj Priyaranjan Kunal ◽  
◽  
Girish Kumar Girish Kumar ◽  
Maria Rosalia Menezes
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Rflp Analysis ◽  
Yellowfin Tuna ◽  
Thunnus Albacares ◽  
Indian Coast ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop

Polymorphisms of genes required for methionine synthesis and DNA methylation influence mitochondrial DNA methylation

Epigenomics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1003-1012
Author(s):  
Andrea Stoccoro ◽  
Pierpaola Tannorella ◽  
Lucia Migliore ◽  
Fabio Coppedè
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Exploratory Study ◽  
High Resolution Melting ◽  
Late Onset ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop ◽  
One Carbon Metabolism ◽  
Methionine Synthesis

Aim: Impaired methylation of the mitochondrial DNA and particularly in the regulatory displacement loop (D-loop) region, is increasingly observed in patients with neurodegenerative disorders. The present study aims to investigate if common polymorphisms of genes required for one-carbon metabolism ( MTHFR, MTRR, MTR and RFC-1) and DNA methylation reactions ( DNMT1, DNMT3A and DNMT3B) influence D-loop methylation levels. Materials & methods: D-loop methylation data were available from 133 late-onset Alzheimer’s disease patients and 130 matched controls. Genotyping was performed with PCR-RFLP or high resolution melting techniques. Results: Both MTRR 66A > G and DNMT3A -448A > G polymorphisms were significantly associated with D-loop methylation levels. Conclusion: This exploratory study suggests that MTRR and DNMT3A polymorphisms influence mitochondrial DNA methylation; further research is required to better address this issue.

PCR-based genetic identification of marlin and other billfish

1998 ◽  
Vol 49 (5) ◽  
pp. 383 ◽  
Author(s):  
B. H. Innes ◽  
P. M. Grewe ◽  
R. D. Ward
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Test ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Genetic Identification ◽  
Dna Molecule ◽  
Pcr Rflp ◽  
D Loop ◽  
Specific Variation ◽  
Species Specific

A genetic test was developed for the identification of the six species of billfish found in Australian waters (black marlin, Indo–Pacific blue marlin, striped marlin, Indo–Pacific sailfish, shortbill spearfish and broadbill swordfish). The test was based on the PCR–RFLP analysis of a 1400 bp region of the mitochondrial DNA molecule, the d-loop, using four restriction enzymes (Hinf I, Rsa I and Sau3A I andTaq I). A total of 33 composite haplotypes were observed among 160 fish; all were species-specific. Three of the species—black marlin, striped marlin and broadbill swordfish—showed sufficient intra-specific variation to be useful in population structure analyses.

scholarly journals Genetic Relationships of Cattle Breeds Assessed by PCR-RFLP of the Bovine Mitochondrial DNA D-loop Region

2005 ◽  
Vol 18 (10) ◽  
pp. 1368-1374 ◽  
Author(s):  
Du Hak Yoon ◽  
Hak Kyo Lee ◽  
Sung Jung Oh ◽  
Ki Chang Hong ◽  
Gwang Joo Jeon ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Relationships ◽  
Cattle Breeds ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop
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