The present study was taken to prepare barley and oat meal supplemented flours with specialreference to chapati making quality. For this purpose, nine treatments of supplemented flours were preparedby gradually replacing whole wheat. Chapaties were prepared from all compositions along with control(100% whole wheat flour) and analysed for dietary fibre content and sensory attributes like colour, taste,aroma, texture, breakability, folding ability, chewability and overall acceptability at stated intervals. Efficacystudy was carried out on healthy individuals to explore the hypocholesterolemic and hypoglycemic worthof chapaties prepared from supplemented flours. Results of dietary fibre analysis showed that there wassignificant increase in level of soluble 0.96 to 3.13%, insoluble 1.92 to 5.57% and total dietary fibre content2.88 to 8.13% with increase in the supplementation level of barley and oat meal. The highest soluble (3.13),insoluble (5.57) and total dietary fibre (8.13) content were found in wheat flour supplemented with 7.5%oat meal and 7.5% barley flour while their concentration was not changed during storage. Sensory attributesshowed that chapaties prepared from wheat flour supplemented with 7.5% oat meal and 7.5% barley flourwere liked the most due to better overall acceptability. On the basis of nutritional and sensory characteristics,three best chapaties along with control were served to normal humans. The results revealed that consumption of chapaties supplemented with 15% oat meal greatly reduced serum cholesterol (7.7%), low density lipoprotein (6.8%), triglycerides (28.5%), blood glucose (5.5%) and weight (4.7%) while increased highdensity lipoprotein (2.0%), serum protein (12.5%) and albumin protein (15.7%) as compared to other twochapaties. It is concluded that the consumption of barley and oat meal supplemented chapaties tackle thehyperglycemia and hypercholesterolemia in healthy humans as well as in obese persons because it deliverthree times more dietary fibre as compared to chapaties prepared from wheat flour only.