Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of microorganisms. Colony-count technique at 30°C

Keyword(s):  
2012 ◽  
Vol 66 (3-4) ◽  
pp. 219-231
Author(s):  
Ljiljana Jankovic ◽  
Brana Radenkovic-Damnjanovic ◽  
Nedjeljko Karabasil ◽  
Milorad Mirilovic ◽  
Slobodan Maric

The objective of this work was to establish, on the grounds of obtained results for the total number of bacteria before and after completed disinfection, whether there are differences in the efficiency of disinfection performed by a professional and disinfection carried out by an unqualified employee in a private slaughterhouse. The material used in these investigations were samples of wet-dry swabs taken over a course of five weeks, before and after disinfection carried out by an unqualified employee and the skilled professional, from the following: the knife used for evisceration, the floor in the evisceration area, from the table serving for bristle removal, and from the floor underneath the bristle removal surface. The wet-dry swabs were taken according to the procedure described in the standard method ISO 18593 (Microbiology of food and animal feeding stuffs - Horizontal methods for sampling techniques from surfaces using contact plates and swabs). Analyzing the taken samples, the total number of bacteria was determined using the standard method ISO 4833 (Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30?C). Disinfection was carried out using a chlorine preparation (sodium dichloroisocyanurate dihydrate) in a concentration of 0.02% and for an exposure period of 30 min. The results were interpreted on the grounds of the border values in evaluating the hygiene of the equipment, tools, and work surfaces, presented in Commission Decision 471/2001/ EC. The results of the investogations have shown that the disinfection performed by the skilled professional was more efficient than the disinfection performed by the unqualified person, as the total number of bacteria was significantly smaller (p<0.01) in the course of all 5 experimental weeks on the evisceration knife, the floor under the bristle removal surface, during weeks 1, 2 and 5, and on the floor in the evisceration area in weeks 1 and 5.


2020 ◽  
Vol 103 (2) ◽  
pp. 513-522
Author(s):  
Patrick Bird ◽  
Benjamin Bastin ◽  
Nicole Klass ◽  
Erin Crowley ◽  
James Agin ◽  
...  

Abstract Background The 3M™ Petrifilm™ Rapid E. coli/Coliform Count Plate is a selective and differential sample-ready-culture medium designed for the rapid enumeration of Escherichia coli (E. coli) and coliforms in the food and beverage industries. Objective The 3M Petrifilm Rapid E. coli/Coliform Count Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual (BAM) Chapter 4 Enumeration of Escherichia coli and the Coliform Bacteria, the International Organization of Standards (ISO) 4832:2006 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coliforms—Colony-count technique, and ISO 16649-2:2017 Microbiology of food and animal feeding stuffs—Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli—Part 2 Colony-count technique at 44 degrees C using bromo-4-chloro-3- indolyl beta-D-glucuronide methods for the enumeration of E. coli and coliforms in dry dog kibble. Method The candidate method was evaluated using two diluents, Butterfield's phosphate buffered diluent and peptone salt solution, in a paired study design with each reference method in a multi-laboratory collaborative study following the current AOAC Validation Guidelines. Three target contamination levels and an uninoculated control level were evaluated. Results The candidate and reference methods were not statistically different at each contamination level. Reproducibility values obtained during the collaborative study were similar between the candidate and reference methods. Conclusion These results demonstrate that the candidate method is equivalent to the reference methods. Highlight 3M Petrifilm Rapid E. coli/Coliform Count Plate was recommended for Official First Action status for enumeration of E. coli and coliforms in a broad range of foods and environmental surfaces.


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