Abstract
Background
In this international Multi Laboratory Trial (MLT) the precision data and the accuracy of the ISO/CD 22184 IDF/WD 244 analytical method for the quantification of 6 different mono- and disaccharides (galactose, glucose, fructose, sucrose, lactose and maltose) in milk and milk products with high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) are established.
Objective
Thirteen different laboratories participated (located in New Zealand, United States, Europe). The study was divided into two parts. Firstly, the learning/familiarization part in which the participants got experienced with the method. After successfully completing this part, the participants were allowed to continue with the second part of this trial: the analyses of the seven blind MLT duplicate samples.
Method
The selected seven MLT samples comprise five MUVA reference dairy samples, one NIST certified infant formula and one industrial sample. In the Tables S-1 to S-6 the results of this MLT study have been summarized per sugar. There is a very good agreement between the MUVA and NIST certified sugar contents in the MLT samples and the in the MLT established sugar contents in those MUVA and NIST samples.
Results
This demonstrates that the accuracy of the newly developed HPAEC-PAD method is very good.
Conclusions
Certificates of analysis were available for both the MUVA samples and the NIST sample. There is a very good agreement between the by MUVA and NIS certified sugar contents in the MLT samples and the corresponding in the MLT established sugar contents. This demonstrates that the accuracy of the HPAEC-PAD method is very good.
In the IDF/ISO Analytical Week in June 2019 in Prague the report on the MLT study were discussed and adopted.
SIMULTANEOUS SEPARATION AND QUANTITATIVE DETERMINATION OF MONOSACCHARIDES, URONIC ACIDS, AND ALDONIC ACIDS BY HIGH PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY COUPLED WITH PULSED AMPEROMETRIC DETECTION IN CORN STOVER PREHYDROLYSATES
