Faculty Opinions recommendation of Determining the genetic structure of the natural population of Staphylococcus aureus: a comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing.

2003 ◽  
Author(s):  
Marilyn Roberts
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Structure ◽  
Natural Population ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Dna Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Phage Typing ◽  
Randomly Amplified Polymorphic Dna ◽  
Pulsed Field

scholarly journals Coagulase gene variants associated with distinct populations of Staphylococcus aureus

2003 ◽  
Vol 130 (2) ◽  
pp. 207-219 ◽  
Author(s):  
P. E. CARTER ◽  
K. BEGBIE ◽  
F. M. THOMSON-CARTER
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Protein A ◽  
Pulsed Field Gel Electrophoresis ◽  
Gene Variants ◽  
Pfge Analysis ◽  
Pulsed Field ◽  
Clonal Distribution

An identifying characteristic of Staphylococcus aureus is the production of staphylocoagulase (coagulase). The aim of this study was to determine the clonal distribution of coagulase gene (coa) variants within populations of S. aureus defined by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and protein A variation. The N-terminal region of the coa gene from 43 methicillin-susceptible (MSSA) and 252 methicillin-resistant (MRSA) S. aureus human isolates and 9 animal S. aureus isolates was amplified and digested with HinfI. Twelve types were identified amongst the MSSA isolates and the majority (93%) of MRSA isolates were assigned to 5 of the 12 types. MLST and PFGE analysis identified epidemic populations of MRSA and each epidemic population was characterized by a different coagulase type. Nine of the 12 MLST-defined clonal complex ancestral genotypes recently described each carried a different coagulase type suggesting that coagulase evolution and the evolution of the clonal complexes are intimately related.

Emergence and Characterization of Foodborne Methicillin-Resistant Staphylococcus aureus in Korea

2010 ◽  
Vol 73 (12) ◽  
pp. 2285-2290 ◽  
Author(s):  
CHAE HONG RHEE ◽  
GUN-JO WOO
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Food Samples ◽  
Pulsed Field Gel Electrophoresis ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Fish Samples ◽  
Toxic Shock Syndrome Toxin ◽  
Mrsa Strains

A total of 165 Staphylococcus aureus strains, isolated from different food samples between 2003 and 2006, were tested for antimicrobial susceptibility. The mecA-positive methicillin-resistant S. aureus (MRSA) strains were further characterized by testing for various virulence genes and by molecular typing with multilocus sequence typing and pulsed-field gel electrophoresis. Of the 165 S. aureus isolates, 150 strains (90.9%) were resistant to at least one antibiotic while no strain was resistant to vancomycin. Four strains were resistant to both oxacillin and cefoxitin and were mecA positive. The mecA-positive MRSA strains were isolated from raw meat and fish samples (two beef samples and two fish samples) and were resistant to β-lactam antibiotics. Based on multilocus sequence typing analysis, the isolates were assigned to sequence type 1 (ST1), ST72, and an undetermined ST (ST72 slv). All four MRSA isolates were shown to be enterotoxigenic. The ST1 MRSA isolate harbored the sea-seh gene combination and the ST72 and ST72 slv MRSA strains harbored the seg-sei and the sea-seg-sei gene combinations, respectively. However, none of the MRSA isolates had the genes for Panton-Valentine leukocidin, toxic shock syndrome toxin 1, and exfoliative toxins. The pulsed-field gel electrophoresis patterns of the ST72 isolates in our study were highly similar, even though they were isolated from food samples in different years and from different regions of Korea.

scholarly journals Comparison of Randomly Amplified Polymorphic DNA Analysis and Pulsed-Field Gel Electrophoresis for Typing of Moraxella catarrhalis Strains

1999 ◽  
Vol 37 (2) ◽  
pp. 450-452 ◽  
Author(s):  
Hoang Vu-Thien ◽  
Carole Dulot ◽  
Didier Moissenet ◽  
Brigitte Fauroux ◽  
Antoine Garbarg-Chenon
Keyword(s):  
Gel Electrophoresis ◽  
Moraxella Catarrhalis ◽  
Dna Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Randomly Amplified Polymorphic Dna ◽  
Pulsed Field ◽  
Chronic Colonization ◽  
Different Strains

Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typing ofM. catarrhalis.

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