Faculty Opinions recommendation of Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586.

2003 ◽  
Author(s):  
Ben Lugtenberg
Keyword(s):  
Pseudomonas Fluorescens ◽  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Characterization of the Mupirocin Biosynthesis Gene Cluster from Pseudomonas fluorescens NCIMB 10586

2003 ◽  
Vol 10 (5) ◽  
pp. 419-430 ◽  
Author(s):  
A.Kassem El-Sayed ◽  
Joanne Hothersall ◽  
Sian M. Cooper ◽  
Elton Stephens ◽  
Thomas J. Simpson ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Characterization of the Biosynthesis Gene Cluster for the Pyrrole Polyether Antibiotic Calcimycin (A23187) inStreptomyces chartreusisNRRL 3882

2010 ◽  
Vol 55 (3) ◽  
pp. 974-982 ◽  
Author(s):  
Qiulin Wu ◽  
Jingdan Liang ◽  
Shuangjun Lin ◽  
Xiufen Zhou ◽  
Linquan Bai ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Mutational Analysis ◽  
Divalent Cations ◽  
Biological Effects ◽  
Biosynthetic Gene Cluster ◽  
Type I ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Hydroxyanthranilic Acid

ABSTRACTThe pyrrole polyether antibiotic calcimycin (A23187) is a rare ionophore that is specific for divalent cations. It is widely used as a biochemical and pharmacological tool because of its multiple, unique biological effects. Here we report on the cloning, sequencing, and mutational analysis of the 64-kb biosynthetic gene cluster fromStreptomyces chartreusisNRRL 3882. Gene replacements confirmed the identity of the gene cluster, andin silicoanalysis of the DNA sequence revealed 27 potential genes, including 3 genes for the biosynthesis of the α-ketopyrrole moiety, 5 genes that encode modular type I polyketide synthases for the biosynthesis of the spiroketal ring, 4 genes for the biosynthesis of 3-hydroxyanthranilic acid, anN-methyltransferase tailoring gene, a resistance gene, a type II thioesterase gene, 3 regulatory genes, 4 genes with other functions, and 5 genes of unknown function. We propose a pathway for the biosynthesis of calcimycin and assign the genes to the biosynthesis steps. Our findings set the stage for producing much desired calcimycin derivatives using genetic modification instead of chemical synthesis.

scholarly journals Isolation and Characterization of Canthaxanthin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobiumsp. Strain ORS278

2000 ◽  
Vol 182 (13) ◽  
pp. 3850-3853 ◽  
Author(s):  
Laure Hannibal ◽  
Jean Lorquin ◽  
Nicolas Angles D'Ortoli ◽  
Nelly Garcia ◽  
Clemence Chaintreuil ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Photosynthetic Bacterium ◽  
Carotenoid Biosynthesis ◽  
Open Reading Frame ◽  
Biosynthesis Gene Cluster ◽  
Reading Frame ◽  
Functional Assignment ◽  
Isolation And Characterization ◽  
Biosynthesis Gene

ABSTRACT A carotenoid biosynthesis gene cluster involved in canthaxanthin production was isolated from the photosyntheticBradyrhizobium sp. strain ORS278. This cluster includes five genes identified as crtE, crtY,crtI, crtB, and crtW that are organized in at least two operons. The functional assignment of each open reading frame was confirmed by complementation studies.

Identification and characterization of the ficellomycin biosynthesis gene cluster from Streptomyces ficellus

2017 ◽  
Vol 101 (20) ◽  
pp. 7589-7602 ◽  
Author(s):  
Yang Liu ◽  
Meng Li ◽  
Huiyan Mu ◽  
Shuting Song ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Identification And Characterization

scholarly journals Characterization of the Final Two Genes of the Gibberellin Biosynthesis Gene Cluster ofGibberella fujikuroi

2003 ◽  
Vol 278 (31) ◽  
pp. 28635-28643 ◽  
Author(s):  
Bettina Tudzynski ◽  
Martina Mihlan ◽  
María Cecilia Rojas ◽  
Pia Linnemannstöns ◽  
Paul Gaskin ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Gibberellin Biosynthesis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Characterization of the Xiamenmycin Biosynthesis Gene Cluster in Streptomyces xiamenensis 318

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99537 ◽  
Author(s):  
Yong Yang ◽  
Ling Fu ◽  
Jinlong Zhang ◽  
Linghan Hu ◽  
Minjuan Xu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene
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