Faculty Opinions recommendation of Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight.

A coiled coil-nucleotide binding site-leucine-rich repeat gene RB/Rpi-blb1 isolated from Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans and is currently employed in potato breeding for durable late blight (LB) resistance. RB homologues were reported in several Solanum species; some of them retained defence function. Here, we report additional evidence on RB-like sequences in 21 Solanum species of the section Petota. The panel of Solanum species was screened with three RB-related PCR markers. RB-like sequences were found in every tested Solanum accession, suggesting universal distribution of RB structural homologues among Solanum genomes, while locus-specific RB-629 was found only in 15 species. Phylogenetic analysis of RB-629 sequences suggested a highly conservative pattern of polymorphisms that was neither species- nor series-specific. Apparently, duplication and evolution of RB-like loci preceded Solanum speciation. Marker presence and particular haplotypes were not immediately associated with high LB resistance.

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Phytophthora infestans Isolates Lacking Class I ipiO Variants Are Virulent on Rpi-blb1 Potato

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-22-12-1535 ◽

2009 ◽

Vol 22 (12) ◽

pp. 1535-1545 ◽

Cited By ~ 77

Author(s):

Nicolas Champouret ◽

Klaas Bouwmeester ◽

Hendrik Rietman ◽

Theo van der Lee ◽

Chris Maliepaard ◽

...

Keyword(s):

Cell Death ◽

Late Blight ◽

Phytophthora Infestans ◽

Broad Spectrum ◽

Late Blight Resistance ◽

Class I ◽

Large Set ◽

Class Iii ◽

Solanum Bulbocastanum ◽

Blight Disease

A strategy to control the devastating late blight disease is providing potato cultivars with genes that are effective in resistance to a broad spectrum of Phytophthora infestans isolates. Thus far, most late blight resistance (R) genes that were introgressed in potato were quickly defeated. In contrast, the Rpi-blb1 gene originating from Solanum bulbocastanum has performed as an exclusive broad-spectrum R gene for many years. Recently, the RXLR effector family ipiO was identified to contain Avr-blb1. Monitoring the genetic diversity of the ipiO family in a large set of isolates of P. infestans and related species resulted in 16 ipiO variants in three distinct classes. Class I and class II but not class III ipiO variants induce cell death when coinfiltrated with Rpi-blb1 in Nicotiana benthamiana. Class I is highly diverse and is represented in all analyzed P. infestans isolates except two Mexican P. infestans isolates, and these were found virulent on Rpi-blb1 plants. In its C-terminal domain, IPI-O contains a W motif that is essential for triggering Rpi-blb1–mediated cell death and is under positive selection. This study shows that profiling the variation of Avr-blb1 within a P. infestans population is instrumental for predicting the effectiveness of Rpi-blb1–mediated resistance in potato.

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Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Frontiers in Plant Science ◽

10.3389/fpls.2020.00699 ◽

2020 ◽

Vol 11 ◽

Author(s):

Elena Rakosy-Tican ◽

Ramona Thieme ◽

Janine König ◽

Marion Nachtigall ◽

Thilo Hammann ◽

...

Keyword(s):

Late Blight ◽

Resistance Genes ◽

Broad Spectrum ◽

Late Blight Resistance ◽

Blight Resistance ◽

R Genes ◽

Solanum Bulbocastanum ◽

Breeding Lines

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Preliminary Results Regarding Presence of RB Genes Homologues from Solanum Bulbocastanum in some Solanum Species

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:6415 ◽

2011 ◽

Vol 68 (1) ◽

Author(s):

Erika BALAZS ◽

Constantin BOTEZ ◽

Abdelmoumen TAOUTAOU

Keyword(s):

Late Blight ◽

Broad Spectrum ◽

Fungal Pathogen ◽

Marker Assisted Selection ◽

Solanum Species ◽

Resistant Variety ◽

Solanum Bulbocastanum ◽

Causal Organism ◽

Resistant Varieties ◽

Rb Gene

Phytophtora infestans, the causal organism of late blight, is the most important fungal pathogen of potato. In the strategy of potato late blight control utilization of resistant variety is the most important. For creating new resistant varieties Marker Assisted Selection increase significant the efficiency of this process. Genomes of several Solanum species were reported to contain RB homologues with confirmed broad-spectrum defence function. We tested 8 primers for RB gene homologues from Solanum bulbocastanum on different potato species, including S. bulbocastanum, S. demissum, and S. tuberosum. Some primers amplified only S. bulbocastanum, and others amplified olso S. demissum and S. tuberosum.

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