Faculty Opinions recommendation of Binding of fluorescent dye to genomic RNA inside intact human rhinovirus after viral capsid penetration investigated by capillary electrophoresis.

2004 ◽  
Author(s):  
David Evans
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescent Dye ◽  
Human Rhinovirus ◽  
Viral Capsid ◽  
Genomic Rna

Binding of Fluorescent Dye to Genomic RNA Inside Intact Human Rhinovirus after Viral Capsid Penetration Investigated by Capillary Electrophoresis

2004 ◽  
Vol 76 (4) ◽  
pp. 882-887 ◽  
Author(s):  
Leopold Kremser ◽  
Vadim M. Okun ◽  
Andreas Nicodemou ◽  
Dieter Blaas ◽  
Ernst Kenndler
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescent Dye ◽  
Human Rhinovirus ◽  
Viral Capsid ◽  
Genomic Rna

scholarly journals Trimeric Hantavirus Nucleocapsid Protein Binds Specifically to the Viral RNA Panhandle

2004 ◽  
Vol 78 (15) ◽  
pp. 8281-8288 ◽  
Author(s):  
M. A. Mir ◽  
A. T. Panganiban
Keyword(s):  
Nucleocapsid Protein ◽  
Rna Binding ◽  
Rna Viruses ◽  
Sin Nombre Virus ◽  
Viral Capsid ◽  
Genomic Rna ◽  
Rna Molecules ◽  
N Protein ◽  
High Affinity ◽  
Negative Sense

ABSTRACT Hantaviruses are tripartite negative-sense RNA viruses and members of the Bunyaviridae family. The nucleocapsid (N) protein is encoded by the smallest of the three genome segments (S). N protein is the principal structural component of the viral capsid and is central to the hantavirus replication cycle. We examined intermolecular N-protein interaction and RNA binding by using bacterially expressed Sin Nombre virus N protein. N assembles into di- and trimeric forms. The mono- and dimeric forms exist transiently and assemble into a trimeric form. In contrast, the trimer is highly stable and does not efficiently disassemble into the mono- and dimeric forms. The purified N-protein trimer is able to discriminate between viral and nonviral RNA molecules and, interestingly, recognizes and binds with high affinity the panhandle structure composed of the 3′ and 5′ ends of the genomic RNA. In contrast, the mono- and dimeric forms of N bind RNA to form a complex that is semispecific and salt sensitive. We suggest that trimerization of N protein is a molecular switch to generate a protein complex that can discriminate between viral and nonviral RNA molecules during the early steps of the encapsidation process.

Fluorescence Labeling of Human Rhinovirus Capsid and Analysis by Capillary Electrophoresis

2004 ◽  
Vol 76 (14) ◽  
pp. 4175-4181 ◽  
Author(s):  
Leopold Kremser ◽  
Tünde Konecsni ◽  
Dieter Blaas ◽  
Ernst Kenndler
Keyword(s):  
Capillary Electrophoresis ◽  
Human Rhinovirus ◽  
Fluorescence Labeling

scholarly journals RNA-protein interactions directed by the 3' end of human rhinovirus genomic RNA.

1995 ◽  
Vol 69 (6) ◽  
pp. 3605-3614 ◽  
Author(s):  
S Todd ◽  
J H Nguyen ◽  
B L Semler
Keyword(s):  
Protein Interactions ◽  
Human Rhinovirus ◽  
Genomic Rna

Building a viral capsid in the presence of genomic RNA

2013 ◽  
Vol 87 (2) ◽  
Author(s):  
Eric C. Dykeman ◽  
Peter G. Stockley ◽  
Reidun Twarock
Keyword(s):  
Viral Capsid ◽  
Genomic Rna

Docking of a human rhinovirus neutralizing antibody onto the viral capsid

1995 ◽  
Vol 23 (4) ◽  
pp. 491-501 ◽  
Author(s):  
José Tormo ◽  
Nuria B. Centeno ◽  
Emilia Fontana ◽  
Thomas Bubendorfer ◽  
Ignacio Fita ◽  
...  
Keyword(s):  
Neutralizing Antibody ◽  
Human Rhinovirus ◽  
Viral Capsid

scholarly journals Species-Specific and Cross-Reactive IgG1 Antibody Binding to Viral Capsid Protein 1 (VP1) Antigens of Human Rhinovirus Species A, B and C

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e70552 ◽  
Author(s):  
Jua Iwasaki ◽  
Wendy-Anne Smith ◽  
Shane R. Stone ◽  
Wayne R. Thomas ◽  
Belinda J. Hales
Keyword(s):  
Capsid Protein ◽  
Antibody Binding ◽  
Human Rhinovirus ◽  
Viral Capsid ◽  
Viral Capsid Protein ◽  
Igg1 Antibody ◽  
Species Specific
Sign in / Sign up

Export Citation Format

Share Document