Faculty Opinions recommendation of RabGEF1 is a negative regulator of mast cell activation and skin inflammation.

Ethnopharmacological Relevance. In this study, we investigated the effects of Tribulus terrestris fruit (Leguminosae, Tribuli Fructus, TF) extract on oxazolone-induced atopic dermatitis in mice. Materials and Methods. TF extract was prepared with 30% ethanol as solvent. The 1% TF extract with or without 0.1% HC was applied to the back skin daily for 24 days. Results. 1% TF extract with 0.1% HC improved AD symptoms and reduced TEWL and symptom scores in AD mice. 1% TF extract with 0.1% HC inhibited skin inflammation through decrease in inflammatory cells infiltration as well as inhibition of Orai-1 expression in skin tissues. TF extract inhibited Orai-1 activity in Orai-1-STIM1 cooverexpressing HEK293T cells but increased TRPV3 activity in TRPV3-overexpressing HEK293T cells. TF extract decreased β-hexosaminidase release in RBL-2H3 cells. Conclusions. The present study demonstrates that the topical application of TF extract improves skin inflammation in AD mice, and the mechanism for this effect appears to be related to the modulation of calcium channels and mast cell activation. This outcome suggests that the combination of TF and steroids could be a more effective and safe approach for AD treatment.

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Extracellular superoxide dismutase ameliorates house dust mite-induced atopic dermatitis-like skin inflammation and inhibits mast cell activation in mice

Experimental Dermatology ◽

10.1111/exd.13028 ◽

2016 ◽

Vol 25 (8) ◽

pp. 630-635 ◽

Cited By ~ 5

Author(s):

Yun Sang Lee ◽

Jung-Hye Choi ◽

Ji-Hyun Lee ◽

Han-Woong Lee ◽

Weontae Lee ◽

...

Keyword(s):

Superoxide Dismutase ◽

Atopic Dermatitis ◽

Mast Cell ◽

House Dust ◽

House Dust Mite ◽

Cell Activation ◽

Skin Inflammation ◽

Mast Cell Activation ◽

Extracellular Superoxide Dismutase ◽

Dust Mite

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Lack of Endogenous Annexin A1 Increases Mast Cell Activation and Exacerbates Experimental Atopic Dermatitis

Cells ◽

10.3390/cells8010051 ◽

2019 ◽

Vol 8 (1) ◽

pp. 51 ◽

Cited By ~ 5

Author(s):

Jéssica Parisi ◽

Mab Corrêa ◽

Cristiane Gil

Keyword(s):

Atopic Dermatitis ◽

Mast Cell ◽

Cell Activation ◽

Skin Diseases ◽

Skin Inflammation ◽

Skin Lesions ◽

Mast Cell Activation ◽

Skin Thickness ◽

Annexin A1 ◽

Inflammatory Skin Diseases

Annexin A1 (AnxA1) is a protein with potent anti-inflammatory actions and an interesting target that has been poorly explored in skin inflammation. This work evaluated the lack of endogenous AnxA1 in the progression of ovalbumin (OVA)-induced atopic dermatitis (AD)-like skin lesions. OVA/Alum-immunized C57BL/6 male wild-type (WT) and AnxA1 null (AnxA1-/-) mice were challenged with drops containing OVA on days 11, 14–18 and 21–24. The AnxA1-/- AD group exhibited skin with intense erythema, erosion and dryness associated with increased skin thickness compared to the AD WT group. The lack of endogenous AnxA1 also increased IgE relative to WT animals, demonstrating exacerbation of the allergic response. Histological analysis revealed intense eosinophilia and mast-cell activation in AD animals, especially in AnxA1-/-. Both AD groups increased skin interleukin (IL)-13 levels, while IL-17A was upregulated in AnxA1-/- lymph nodes and mast cells. High levels of phosphorylated ERK were detected in keratinocytes from AD groups. However, phospho-ERK levels were higher in the AnxA1-/- when compared to the respective control groups. Our results suggest AnxA1 as an important therapeutic target for inflammatory skin diseases.

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