Faculty Opinions recommendation of Retinoic acid controls the bilateral symmetry of somite formation in the mouse embryo.

Orthotopic grafts of wheat germ agglutinin-colloidal gold conjugate (WGA-gold) labelled cells were used to demonstrate differences in the segmental fate of cells in the presomitic mesoderm of the early-somite-stage mouse embryos developing in vitro. Labelled cells in the anterior region of the presomitic mesoderm colonized the first three somites formed after grafting, while those grafted to the middle region of this tissue were found mostly in the 4th-7th newly formed somites. Labelled cells grafted to the posterior region were incorporated into somites whose somitomeres were not yet present in the presomitic mesoderm at the time of grafting. There was therefore an apparent posterior displacement of the grafted cells in the presomitic mesoderm. Colonization of somites by WGA-gold labelled cells was usually limited to two to three consecutive somites in the chimaera. The distribution of cells derived from a single graft to two somites was most likely due to the segregation of the labelled population when cells were allocated to adjacent meristic units during somite formation. Further spreading of the labelled cells to several somites in some cases was probably the result of a more extensive mixing of mesodermal cells among the somitomeres prior to somite segmentation.

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Retinoic Acid Teratogenicity in Mouse Embryo

Medico-Legal Update ◽

10.37506/mlu.v20i4.1953 ◽

2020 ◽

Vol 20 (4) ◽

Keyword(s):

Retinoic Acid ◽

Mouse Embryo

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Expression of cellular retinoic acid binding protein in the developing nervous system of mouse embryo

Developmental Brain Research ◽

10.1016/0165-3806(90)90138-o ◽

1990 ◽

Vol 54 (2) ◽

pp. 161-167 ◽

Cited By ~ 7

Author(s):

Mariko Momoi ◽

Takanori Yamagata ◽

Kou Ichihashi ◽

Masayoshi Yanagisawa ◽

Makoto Yamakado ◽

...

Keyword(s):

Nervous System ◽

Retinoic Acid ◽

Mouse Embryo ◽

Binding Protein ◽

Acid Binding Protein ◽

Developing Nervous System

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The expression of cellular retinoic acid binding protein (CRABP) in the neural tube and notochord of mouse embryo.

Proceedings of the Japan Academy Series B ◽

10.2183/pjab.65.9 ◽

1989 ◽

Vol 65 (2) ◽

pp. 9-12 ◽

Cited By ~ 3

Author(s):

Mariko Y. MOMOI ◽

Michiko HAYASAKA ◽

Kazunori HANAOKA ◽

Takashi MOMOI

Keyword(s):

Retinoic Acid ◽

Neural Tube ◽

Mouse Embryo ◽

Binding Protein ◽

Acid Binding Protein

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Domains of cellular retinoic acid-binding protein I (CRABP I) expression in the hindbrain and neural crest of the mouse embryo

Mechanisms of Development ◽

10.1016/0925-4773(92)90011-8 ◽

1992 ◽

Vol 37 (1-2) ◽

pp. 13-23 ◽

Cited By ~ 71

Author(s):

Malcolm Maden ◽

Claire Horton ◽

Anthony Graham ◽

Lisa Leonard ◽

John Pizzey ◽

...

Keyword(s):

Retinoic Acid ◽

Neural Crest ◽

Mouse Embryo ◽

Binding Protein ◽

Acid Binding Protein ◽

Crabp I

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A new coactivator complex required for retinoic acid-dependent regulation of embryonic symmetry

10.1101/089201 ◽

2016 ◽

Author(s):

Goncalo C. Vilhais-Neto ◽

Marjorie Fournier ◽

Jean-Luc Plassat ◽

Mihaela E. Sardiu ◽

Anita Saraf ◽

...

Keyword(s):

Retinoic Acid ◽

Rna Polymerase Ii ◽

Protein Complex ◽

Target Genes ◽

Bilateral Symmetry ◽

Molecular Control ◽

Mouse Mutants ◽

Proteomic Approach

Bilateral symmetry is a striking feature of the vertebrate body plan organization. Vertebral precursors, called somites, provide one of the best illustrations of embryonic symmetry. Maintenance of somitogenesis symmetry requires Retinoic acid (RA) and its coactivator Rere/Atrophin2. Here, using a proteomic approach we identify a protein complex, containing Wdr5, Hdac1, Hdac2 and Rere (named WHHERE), which regulates RA signalling and controls embryonic symmetry. We demonstrate that Wdr5, Hdac1 and Hdac2 are required for RA signalling in vitro and in vivo. Mouse mutants for Wdr5 and Hdac1 exhibit asymmetrical somite formation characteristic of RA-deficiency. We also identify the Rere-binding histone methyltransferase Ehmt2/G9a, as a RA coactivator controlling somite symmetry. Upon RA treatment, WHHERE and Ehmt2 become enriched at RA target genes to promote RNA Polymerase II recruitment. Our work identifies a novel protein complex linking key epigenetic regulators acting in the molecular control of embryonic bilateral symmetry.

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Specific effects of retinoic acid on the skeletal morphogenesis of the 11-day mouse embryo forelimb bud in vitro

Biology of the Cell ◽

10.1016/0248-4900(90)90310-y ◽

1990 ◽

Vol 68 (3) ◽

pp. 213-220 ◽

Cited By ~ 3

Author(s):

Xavier Desbiens ◽

Lucette Meunier ◽

Bernard Lassalle

Keyword(s):

Retinoic Acid ◽

Mouse Embryo ◽

Specific Effects

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Retinoic acid is required in the mouse embryo for left-right asymmetry determination and heart morphogenesis

Development ◽

10.1242/dev.126.12.2589 ◽

1999 ◽

Vol 126 (12) ◽

pp. 2589-2596 ◽

Cited By ~ 10

Author(s):

C. Chazaud ◽

P. Chambon ◽

P. Dolle

Keyword(s):

Retinoic Acid ◽

Vitamin A ◽

Mouse Embryo ◽

Time Course ◽

Retinoic Acid Receptor ◽

Mouse Embryos ◽

Anteroposterior Patterning ◽

Heart Looping ◽

Left Right Asymmetry

Determination of the left-right position (situs) of visceral organs involves lefty, nodal and Pitx2 genes that are specifically expressed on the left side of the embryo. We demonstrate that the expression of these genes is prevented by the addition of a retinoic acid receptor pan-antagonist to cultured headfold stage mouse embryos, whereas addition of excess retinoic acid leads to their symmetrical expression. Interestingly, both treatments lead to randomization of heart looping and to defects in heart anteroposterior patterning. A time course analysis indicates that only the newly formed mesoderm at the headfold-presomite stage is competent for these retinoid effects. We conclude that retinoic acid, the active derivative of vitamin A, is essential for heart situs determination and morphogenesis.

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