Faculty Opinions recommendation of Unique maturation program of the IgE response in vivo.

2007 ◽  
Author(s):  
P'ng Loke
Keyword(s):  
Ige Response

scholarly journals Hyper Immunoglobulin E Response in Mice with Monoclonal Populations of B and T Lymphocytes1✪

2001 ◽  
Vol 194 (9) ◽  
pp. 1349-1360 ◽  
Author(s):  
Maria A. Curotto de Lafaille ◽  
Stephanie Muriglan ◽  
Mary-Jean Sunshine ◽  
Ying Lei ◽  
Nino Kutchukhidze ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Immunoglobulin E ◽  
Effector Memory ◽  
Serum Ige ◽  
Activation Induced Cell Death ◽  
Normal Individuals ◽  
Specific Effector ◽  
Ige Response

A key event in the pathogenesis of allergies is the production of antibodies of the immunoglobulin (Ig)E class. In normal individuals the levels of IgE are tightly regulated, as illustrated by the low serum IgE concentration. In addition, multiple immunizations are usually required to generate detectable IgE responses in normal experimental animals. To define the parameters that regulate IgE production in vivo, we generated mice bearing monoclonal populations of B and T lymphocytes specific for influenza virus hemagglutinin (HA) and chicken ovalbumin (OVA), respectively. A single immunization of the monoclonal mice with the cross-linked OVA-HA antigen led to serum IgE levels that reached 30–200 μg/ml. This unusually high IgE response was prevented by the infusion of regulatory α/β CD4+ T cells belonging to both CD25+ and CD25− subpopulations. The regulation by the infused T cells impeded the development of fully competent OVA-specific effector/memory Th2 lymphocytes without inhibiting the initial proliferative response of T cells or promoting activation-induced cell death. Our results indicate that hyper IgE responses do not occur in normal individuals due to the presence of regulatory T cells, and imply that the induction of regulatory CD4+ T cells could be used for the prevention of atopy.

scholarly journals Unique Maturation Program of the IgE Response In Vivo

Immunity ◽  
2007 ◽  
Vol 26 (2) ◽  
pp. 191-203 ◽  
Author(s):  
Agustin Erazo ◽  
Nino Kutchukhidze ◽  
Monica Leung ◽  
Ana P. Guarnieri Christ ◽  
Joseph F. Urban ◽  
...  
Keyword(s):  
Ige Response

Inhibition of an in vivo antigen-specific IgE response by antibodies to CD23

Science ◽  
1993 ◽  
Vol 261 (5124) ◽  
pp. 1038-1041 ◽  
Author(s):  
L Flores-Romo ◽  
J Shields ◽  
Y Humbert ◽  
P Graber ◽  
J. Aubry ◽  
...  
Keyword(s):  
Specific Ige ◽  
Ige Response

scholarly journals Specific panallergen peptide of Sorghum Polcalcin showing IgE response identified based on in silico and in vivo peptide mapping

2019 ◽  
Vol 39 (11) ◽  
Author(s):  
Chandra Sekhar Bokka ◽  
Ganesh Kumar Veeramachaneni ◽  
V.B.S.C. Thunuguntla ◽  
Naresh Kumar Manda ◽  
Jayakumar Singh Bondili
Keyword(s):  
In Silico ◽  
Peptide Mapping ◽  
Interferon Γ ◽  
Docking Studies ◽  
Antigenic Determinants ◽  
T Helper ◽  
In Vivo Evaluation ◽  
Ige Response ◽  
Helper Type

Abstract In India, Sorghum plant allergenicity was reported to be approximately 54.9%. Sorghum bicolor Polcalcin (Sorb PC) was identified as the panallergen but the specificity of this allergen is yet to be characterized. The present study was aimed to characterize the antigenic determinants of Sorb PC that are responsible for eliciting the IgE response. In silico modeling, simulation studies and docking of Sorb PC peptides (PC1–11) against IgG and IgE followed by in vivo evaluation was adopted. Peptide docking studies revealed PC 6 with highest G-score −12.85 against IgE followed by PC-11, 5, 1 and 7 (−10.91) peptides. The mice sensitized with PC7 peptide showed interleukin (IL) 4 (IL-4), IL-5, IL-12, TNF-α and GMCSF levels increased when compared with other peptides and controls, signifying a strong T helper type 2 (Th2)-based response. In tandem, the T helper type 1 (Th1) pathway was inhibited by low levels of cytokine IL-2, interferon γ (IFN-γ) and increased IL-10 levels justifying the role of PC7 in allergic IgE response. Considering the above data of overlapping peptides of PC6 and PC7, N-terminal part of the PC7 peptide (DEVQRMM) is found to play a crucial role in Sorghum Polcalcin allergenic response.

scholarly journals The Extracellular Domains of IgG1 and T Cell-Derived IL-4/IL-13 Are Critical for the Polyclonal Memory IgE Response In Vivo

PLoS Biology ◽  
2015 ◽  
Vol 13 (11) ◽  
pp. e1002290 ◽  
Author(s):  
Adriana Turqueti-Neves ◽  
Manuel Otte ◽  
Christian Schwartz ◽  
Michaela Erika Renate Schmitt ◽  
Cornelia Lindner ◽  
...  
Keyword(s):  
T Cell ◽  
Extracellular Domains ◽  
Ige Response

scholarly journals Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 178
Author(s):  
Chandra Sekhar Bokka ◽  
Ganesh Kumar Veeramachaneni ◽  
V. B. S. C. Thunuguntla ◽  
Janakiram Bobbillapati ◽  
Jayakumar Singh Bondili
Keyword(s):  
In Silico ◽  
Peptide Mapping ◽  
Antigenic Determinants ◽  
In Vivo Evaluation ◽  
In Silico Modeling ◽  
In Vivo Analysis ◽  
Low Levels ◽  
Ige Response

Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Methods: To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Results: Peptide PF3 and PF4 displayed high docking G-scores (−9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response. Conclusions: Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.

scholarly journals Inhibition of specific IgE response in vivo by allergen-gene transfer

1996 ◽  
Vol 8 (9) ◽  
pp. 1405-1411 ◽  
Author(s):  
Ching-Hsiang Hsu ◽  
Kaw-Yan Chua ◽  
MI-Hua Tao ◽  
Shau-Ku Huang ◽  
Kue-Hsiung Hsieh ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Specific Ige ◽  
Ige Response

scholarly journals Regulation of reaginic antibody production in mice. I. Suppression by antigen of IgE antibody production in vitro.

1977 ◽  
Vol 146 (6) ◽  
pp. 1534-1548 ◽  
Author(s):  
P J Danneman ◽  
J G Michael
Keyword(s):  
T Lymphocytes ◽  
Antibody Production ◽  
Direct Interaction ◽  
Lymphocyte Culture ◽  
Splenic Lymphocyte ◽  
Ige Antibody ◽  
Antigen System ◽  
Ige Response

Regulation of IgE production by antigen in a primed murine splenic lymphocyte culture system was described. Maximum IgE antibody production was found to occur when cells were cultured in the absence of exogenously added antigen. A cells and T lymphocytes did not affect the production of anti-DNP IgE antibody. By using a hapten-carrier antigen system (DNP-EA) for priming mice in vivo, it was found that the production of anti-DNP IgE by spleen cells in vitro was inhibited by hapten when coupled to homologous (EA) or heterologous (BGG) carrier, and was not enhanced or inhibited by homologous carrier. Anti-DNP IgE antibody production by cultures depleted of macrophages or T lymphocytes was found to be as sensitive to the suppressive effects of hapten as was the IgE production by whole spleen cell cultures. Both IgM and IgG secondary anti-DNP PFC responses in vitro were enhanced by the presence of the homologous hapten-carrier or carrier alone. DNP-BGG had no effect on the anti-DNP IgM or IgG PFC responses of the cultures. These data suggest that endogenous production of antibody (IgM or IgG) was not responsible for the observed suppression of the IgE response in vitrol The experimental results presented indicate that the regulation of the IgE production by antigen in the primed mouse splenic lymphocyte cultures was a consequence of the direct interaction of hapten with IgE B cells.

scholarly journals CD40 and IgE: synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells.

1990 ◽  
Vol 172 (6) ◽  
pp. 1861-1864 ◽  
Author(s):  
H H Jabara ◽  
S M Fu ◽  
R S Geha ◽  
D Vercelli
Keyword(s):  
Monoclonal Antibody ◽  
B Cells ◽  
B Cell ◽  
Interleukin 4 ◽  
B Cell Differentiation ◽  
Human B Cells ◽  
Molecular Events ◽  
Ige Synthesis ◽  
Ige Response

A novel pathway of IgE-B cell differentiation has been identified. Engagement of the B cell antigen CD40 by F(ab')2 fragments of monoclonal antibody (mAb) 626.1 in the presence of recombinant interleukin 4 (rIL-4) induced intense IgE synthesis, but modest IgG synthesis, by highly purified human B cells. Surface IgE- B cells isolated by cell sorting were induced to produce IgE by mAb 626.1 and IL-4. Thus, IgE synthesis is unlikely to result from expansion of a B cell population precommitted to IgE in vivo. A neutralizing anti-IL-6 antibody strongly, but not completely, inhibited the IgE response. This indicates that autocrine production of IL-6 plays an important amplification role in IgE synthesis triggered by anti-CD40 mAb and IL-4. Although the exact role played by CD40 in IgE responses in vivo remains to be established, this T cell-independent system represents a useful model to characterize the biochemical and molecular events leading to IgE synthesis in human B cells.

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