Faculty Opinions recommendation of Regulation of ABCG2 expression at the 3' untranslated region of its mRNA through modulation of transcript stability and protein translation by a putative microRNA in the S1 colon cancer cell line.

2009 ◽  
Author(s):  
Deanna Kroetz ◽  
Mike Baldwin
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Untranslated Region ◽  
Cancer Cell Line ◽  
Protein Translation ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Transcript Stability

scholarly journals Regulation of ABCG2 Expression at the 3′ Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line

2008 ◽  
Vol 28 (17) ◽  
pp. 5147-5161 ◽  
Author(s):  
Kenneth K. W. To ◽  
Zhirong Zhan ◽  
Thomas Litman ◽  
Susan E. Bates
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Untranslated Region ◽  
Mutational Analysis ◽  
Cancer Cell Line ◽  
Protein Translation ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Drug Resistant

ABSTRACT ABCG2 is recognized as an important efflux transporter in clinical pharmacology and is potentially important in resistance to chemotherapeutic drugs. To identify epigenetic mechanisms regulating ABCG2 mRNA expression at its 3′ untranslated region (3′UTR), we performed 3′ rapid amplification of cDNA ends with the S1 parental colon cancer cell line and its drug-resistant ABCG2-overexpressing counterpart. We found that the 3′UTR is >1,500 bp longer in parental cells and, using the miRBase TARGETs database, identified a putative microRNA (miRNA) binding site, distinct from the recently reported hsa-miR520h site, in the portion of the 3′UTR missing from ABCG2 mRNA in the resistant cells. We hypothesized that the binding of a putative miRNA at the 3′UTR of ABCG2 suppresses the expression of ABCG2. In resistant S1MI80 cells, the miRNA cannot bind to ABCG2 mRNA because of the shorter 3′UTR, and thus, mRNA degradation and/or repression on protein translation is relieved, contributing to overexpression of ABCG2. This hypothesis was rigorously tested by reporter gene assays, mutational analysis at the miRNA binding sites, and forced expression of miRNA inhibitors or mimics. The removal of this epigenetic regulation by miRNA could be involved in the overexpression of ABCG2 in drug-resistant cancer cells.

Anticancer effect of X-Ray triggered methotrexate conjugated albumin coated bismuth sulfide nanoparticles on SW480 colon cancer cell line

2020 ◽  
Vol 582 ◽  
pp. 119320
Author(s):  
Mohammad Hasan Faghfoori ◽  
Hamed Nosrati ◽  
Hamed Rezaeejam ◽  
Jalil Charmi ◽  
Saeed Kaboli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Bismuth Sulfide ◽  
Anticancer Effect ◽  
X Ray

scholarly journals Target disruption of the mutant β-catenin gene in colon cancer cell line HCT116: preservation of its malignant phenotype

Oncogene ◽  
2002 ◽  
Vol 21 (38) ◽  
pp. 5906-5911 ◽  
Author(s):  
Shigeki Sekine ◽  
Tatsuhiro Shibata ◽  
Michiie Sakamoto ◽  
Setsuo Hirohashi
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Malignant Phenotype

scholarly journals In situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jigang Wang ◽  
Jianbin Zhang ◽  
Chong-Jing Zhang ◽  
Yin Kwan Wong ◽  
Teck Kwang Lim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Proteomic Profiling ◽  
Hct116 Colon Cancer Cell

scholarly journals A new method for detection of tumor driver-dependent changes of protein sialylation in a colon cancer cell line reveals nectin-3 as TGFBR2 target

2015 ◽  
Vol 24 (10) ◽  
pp. 1686-1694 ◽  
Author(s):  
Jennifer Lee ◽  
Uwe Warnken ◽  
Martina Schnölzer ◽  
Johannes Gebert ◽  
Jürgen Kopitz
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
New Method

scholarly journals Magnesium deficiency with high calcium-to-magnesium ratio promotes a metastatic phenotype in the CT26 colon cancer cell line

2020 ◽  
Vol 33 (3) ◽  
pp. 68-85
Author(s):  
Gopal Kumar ◽  
Prodyot K. Chatterjee ◽  
Swati Madankumar ◽  
Syed F. Mehdi ◽  
Xiangying Xue ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Magnesium Deficiency ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Metastatic Phenotype ◽  
High Calcium

Evaluation of the Effects of the Flavonoid Apigenin on Apoptotic Pathway Gene Expression on the Colon Cancer Cell Line (HT29)

2011 ◽  
Vol 14 (10) ◽  
pp. 1107-1117 ◽  
Author(s):  
Mehmet Turktekin ◽  
Ece Konac ◽  
H. Ilke Onen ◽  
Ebru Alp ◽  
Akin Yilmaz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Apoptotic Pathway ◽  
Pathway Gene
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