scholarly journals In situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jigang Wang ◽  
Jianbin Zhang ◽  
Chong-Jing Zhang ◽  
Yin Kwan Wong ◽  
Teck Kwang Lim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Proteomic Profiling ◽  
Hct116 Colon Cancer Cell

scholarly journals KO of 5-InsP7kinase activity transforms the HCT116 colon cancer cell line into a hypermetabolic, growth-inhibited phenotype

2017 ◽  
Vol 114 (45) ◽  
pp. 11968-11973 ◽  
Author(s):  
Chunfang Gu ◽  
Hoai-Nghia Nguyen ◽  
Douglas Ganini ◽  
Zhaowei Chen ◽  
Henning J. Jessen ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Tumor Therapy ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
P53 Expression ◽  
Hct116 Colon Cancer Cell ◽  
Hct116 Cells

The inositol pyrophosphates 5-InsP7(diphosphoinositol pentakisphosphate) and 1,5-InsP8(bis-diphosphoinositol tetrakisphosphate) are highly energetic cellular signals interconverted by the diphosphoinositol pentakisphosphate kinases (PPIP5Ks). Here, we used CRISPR to KO PPIP5Ks in the HCT116 colon cancer cell line. This procedure eliminates 1,5-InsP8and raises 5-InsP7levels threefold. Expression of p53 and p21 was up-regulated; proliferation and G1/S cell-cycle transition slowed. Thus, PPIP5Ks are potential targets for tumor therapy. Deletion of the PPIP5Ks elevated [ATP] by 35%; both [ATP] and [5-InsP7] were restored to WT levels by overexpression of PPIP5K1, and a kinase-compromised PPIP5K1 mutant had no effect. This covariance of [ATP] with [5-InsP7] provides direct support for an energy-sensing attribute (i.e., 1 mMKmfor ATP) of the 5-InsP7–generating inositol hexakisphosphate kinases (IP6Ks). We consolidate this conclusion by showing that 5-InsP7levels are elevated on direct delivery of ATP into HCT116 cells using liposomes. Elevated [ATP] inPPIP5K−/−HCT116 cells is underpinned by increased mitochondrial oxidative phosphorylation and enhanced glycolysis. To distinguish between 1,5-InsP8and 5-InsP7as drivers of the hypermetabolic and p53-elevated phenotypes, we usedIP6K2RNAi and the pan-IP6K inhibitor,N2-(m-trifluorobenzyl),N6-(p-nitrobenzyl) purine (TNP), to return 5-InsP7levels inPPIP5K−/−cells to those of WT cells without rescuing 1,5-InsP8levels. Attenuation of IP6K restored p53 expression but did not affect the hypermetabolic phenotype. Thus, we conclude that 5-InsP7regulates p53 expression, whereas 1,5-InsP8regulates ATP levels. These findings attribute hitherto unsuspected functionality for 1,5-InsP8to bioenergetic homeostasis.

scholarly journals DNA methyltransferase knockout alters calcium homeostasis in HCT116 colon cancer cell line (1013.4)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Jeffery Rovatti ◽  
Alexander Wu ◽  
Shannon Kinney ◽  
Diptiman Bose
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Calcium Homeostasis ◽  
Dna Methyltransferase ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Hct116 Colon Cancer Cell

scholarly journals Investigation of PTGS2, MAGE-A3, CALR, KRT19 and TMPRSS4 expressions in HCT116 colon cancer and PC3 prostate cancer cell lines

2020 ◽  
Vol 4 (2) ◽  
pp. 37
Author(s):  
Süreyya Bozkurt ◽  
Filiz Yarimcan ◽  
Hüseyin Ayhan ◽  
Hacer Kotan ◽  
Hüma Tuğçe Sezgin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Hct116 Colon Cancer Cell

Cancer is a disease arising from DNA alterations that dysregulate gene structure and function. These deregulated genes can also play a role in tumor invasion and metastasis or resistance to treatment. In this study, we determined the gene expression during transcription of PTGS2 (Prostaglandin-endoperoxide synthase 2), MAGE-A3 (Melanoma-associated antigen 3), CALR (Calreticulin), KRT19 (Cytokeratin 19), and TMPRSS4 (Transmembrane protease, serine 4) in HCT116 colon cancer cell line and PC3 prostate cancer cell line. After RNA isolation and cDNA conversion, DNA amplification was performed with Real-Time PCR. We determined the altered transcriptional expression level of those genes. In HCT116 colon cancer cell line, expression of the TMPRSS4 gene, MAGEA3 gene and KRT19 gene was found as increased and expression of the CALR gene and the PTGS2 gene was found as decreased. Especially a 93.70-fold increase in expression of the KRT19 gene was found in HCT116 colon cancer cell line. In PC3 prostate cancer cell lines, TMPRS4 gene expression and MAGEA3 gene expression were found as increased. But there was 50 fold decrease in PTGS2 gene expression.

scholarly journals Rapamycin synergizes with low-dose oxaliplatin in the HCT116 colon cancer cell line by inducing enhanced apoptosis

2011 ◽  
Vol 2 (4) ◽  
pp. 643-647 ◽  
Author(s):  
XUEYING LU ◽  
HAIBO WEI ◽  
XIAOJIN ZHANG ◽  
WENXIN ZHENG ◽  
CHENG CHANG ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Low Dose ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Hct116 Colon Cancer Cell

Homozygous deletions may be markers of nearby heterozygous mutations: The complex deletion atFRA16Din the HCT116 colon cancer cell line removes exons ofWWOX

2008 ◽  
Vol 47 (5) ◽  
pp. 437-447 ◽  
Author(s):  
Amber E. Alsop ◽  
Karen Taylor ◽  
Jieqing Zhang ◽  
Hani Gabra ◽  
Adam J. W. Paige ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Hct116 Colon Cancer Cell ◽  
Homozygous Deletions

Anticancer effect of X-Ray triggered methotrexate conjugated albumin coated bismuth sulfide nanoparticles on SW480 colon cancer cell line

2020 ◽  
Vol 582 ◽  
pp. 119320
Author(s):  
Mohammad Hasan Faghfoori ◽  
Hamed Nosrati ◽  
Hamed Rezaeejam ◽  
Jalil Charmi ◽  
Saeed Kaboli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Bismuth Sulfide ◽  
Anticancer Effect ◽  
X Ray
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