Faculty Opinions recommendation of Coordination of polymerization, chain termination, and export in assembly of the Escherichia coli lipopolysaccharide O9a antigen in an ATP-binding cassette transporter-dependent pathway.

2009 ◽  
Author(s):  
Miguel Valvano
Keyword(s):  
Escherichia Coli ◽  
Chain Termination ◽  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Dependent Pathway ◽  
Escherichia Coli Lipopolysaccharide ◽  
Polymerization Chain ◽  
Atp Binding Cassette

scholarly journals The MalF P2 Loop of the ATP-Binding Cassette Transporter MalFGK2 from Escherichia coli and Salmonella enterica Serovar Typhimurium Interacts with Maltose Binding Protein (MalE) throughout the Catalytic Cycle

2008 ◽  
Vol 191 (3) ◽  
pp. 754-761 ◽  
Author(s):  
Martin L. Daus ◽  
Mathias Grote ◽  
Erwin Schneider
Keyword(s):  
Escherichia Coli ◽  
Salmonella Enterica ◽  
Binding Protein ◽  
Maltose Binding Protein ◽  
Cross Linking ◽  
Atp Binding ◽  
Cross Link ◽  
Atp Binding Cassette Transporter ◽  
Serovar Typhimurium ◽  
Atp Binding Cassette

ABSTRACT We have investigated the interaction of the uncommonly large periplasmic P2 loop of the MalF subunit of the maltose ATP-binding cassette transporter (MalFGK2) from Escherichia coli and Salmonella enterica serovar Typhimurium with maltose binding protein (MalE) by site-specific chemical cross-linking in the assembled transport complex. We focused on possible distance changes between two pairs of residues of the P2 loop and MalE during the transport cycle. The distance between MalF(S205C) and MalE(T80C) (∼5 Å) remained unchanged under all conditions tested. Cross-linking did not affect the ATPase activity of the complex. The distance between MalF(T177C) and MalE(T31C) changed from ∼10 Å to ∼5 Å upon binding of ATP (or maltose, with a less pronounced result) and was reset to ∼10 Å after hydrolysis of one ATP. A cross-link (∼25 Å) between MalF(S205C) and MalE(T31C) was observed only when the transporter resided in a transition state-like conformation, as was the case after vanadate trapping or in a binding protein-independent mutant, both of which are characterized by tight binding of unliganded MalE to the transporter. Thus, we propose that the observed cross-link is indicative of catalytic intermediates of the transporter. Together, our results strengthen the notion that the MalF P2 loop plays an important role in intersubunit communication. In particular, this loop is involved in keeping MalE in close contact with the transporter. The data are discussed with respect to a crystal structure and current transport models.

Hyperglycemia accelerates ATP-binding cassette transporter A1 degradation via an ERK-dependent pathway in macrophages

2013 ◽  
Vol 114 (6) ◽  
pp. 1364-1373 ◽  
Author(s):  
Yu-Cheng Chang ◽  
Wayne H.-H. Sheu ◽  
Yi-Shen Chien ◽  
Pei-Chi Tseng ◽  
Wen-Jane Lee ◽  
...  
Keyword(s):  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Dependent Pathway ◽  
Atp Binding Cassette
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