Faculty Opinions recommendation of Human epidermal Langerhans cells maintain immune homeostasis in skin by activating skin resident regulatory T cells.

2012 ◽  
Author(s):  
Oscar Colegio ◽  
Rachel Rosenstein
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Langerhans Cells ◽  
Immune Homeostasis ◽  
Epidermal Langerhans Cells

scholarly journals Human Epidermal Langerhans Cells Maintain Immune Homeostasis in Skin by Activating Skin Resident Regulatory T Cells

Immunity ◽  
2012 ◽  
Vol 36 (5) ◽  
pp. 873-884 ◽  
Author(s):  
Julien Seneschal ◽  
Rachael A. Clark ◽  
Ahmed Gehad ◽  
Clare M. Baecher-Allan ◽  
Thomas S. Kupper
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Langerhans Cells ◽  
Immune Homeostasis ◽  
Epidermal Langerhans Cells

In vitro primary sensitization of hapten-specific T cells by cultured human epidermal Langerhans cells - a screening predictive assay for contact sensitizers

1996 ◽  
Vol 26 (5) ◽  
pp. 563-570 ◽  
Author(s):  
M. KRASTEVA ◽  
J. PEGUET-NAVARRO ◽  
C. MOULON ◽  
P. COURTELLEMONT ◽  
G. REDZINIAK ◽  
...  
Keyword(s):  
T Cells ◽  
Langerhans Cells ◽  
Primary Sensitization ◽  
Predictive Assay ◽  
Epidermal Langerhans Cells

scholarly journals Anti-CD25 monoclonal antibody Fc variants differentially impact regulatory T cells and immune homeostasis

Immunology ◽  
2016 ◽  
Vol 148 (3) ◽  
pp. 276-286 ◽  
Author(s):  
David J. Huss ◽  
Alex F. Pellerin ◽  
Brian P. Collette ◽  
Arun K. Kannan ◽  
Liaomin Peng ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Cells ◽  
Regulatory T Cells ◽  
Immune Homeostasis

In cutaneous leishmaniasis, induction of retinoic acid in skin-derived Langerhans cells is not sufficient for induction of parasite persistence-mediating regulatory T cells

2017 ◽  
Vol 87 (3) ◽  
pp. 307-309
Author(s):  
Kirsten Dietze-Schwonberg ◽  
Kordula Kautz-Neu ◽  
Beate Lorenz ◽  
Björn E. Clausen ◽  
Esther von Stebut
Keyword(s):  
T Cells ◽  
Retinoic Acid ◽  
Regulatory T Cells ◽  
Cutaneous Leishmaniasis ◽  
Langerhans Cells

In vitro primary sensitization of hapten-specific T cells by cultured human epidermal Langerhans cells-a screening predictive assay for contact sensitizers

1996 ◽  
Vol 26 (5) ◽  
pp. 563-570 ◽  
Author(s):  
M. KRASTEVA ◽  
J. PEGUET-NAVARRO ◽  
C. MOULON ◽  
P. COURTELLEMONT ◽  
G. REDZINIAK ◽  
...  
Keyword(s):  
T Cells ◽  
Langerhans Cells ◽  
Primary Sensitization ◽  
Predictive Assay ◽  
Epidermal Langerhans Cells

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen-presenting cells that induce proallergic T cells

2007 ◽  
Vol 119 (4) ◽  
pp. 982-990 ◽  
Author(s):  
Susanne Ebner ◽  
Van Anh Nguyen ◽  
Markus Forstner ◽  
Yui-Hsi Wang ◽  
Dolores Wolfram ◽  
...  
Keyword(s):  
T Cells ◽  
Langerhans Cells ◽  
Antigen Presenting Cells ◽  
Thymic Stromal Lymphopoietin ◽  
Antigen Presenting ◽  
Epidermal Langerhans Cells

scholarly journals Fast and Efficient Genome Editing of Human FOXP3+ Regulatory T Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Lauren Van Zeebroeck ◽  
Rebeca Arroyo Hornero ◽  
Beatriz F. Côrte-Real ◽  
Ibrahim Hamad ◽  
Torsten B. Meissner ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Genome Editing ◽  
Therapeutic Target ◽  
Peripheral Tolerance ◽  
Immune Homeostasis ◽  
Cell Integrity ◽  
Potential Therapeutic Target ◽  
Functional Assays

FOXP3+ regulatory T cells (Tregs) are central for maintaining peripheral tolerance and immune homeostasis. Because of their immunosuppressive characteristics, Tregs are a potential therapeutic target in various diseases such as autoimmunity, transplantation and infectious diseases like COVID-19. Numerous studies are currently exploring the potential of adoptive Treg therapy in different disease settings and novel genome editing techniques like CRISPR/Cas will likely widen possibilities to strengthen its efficacy. However, robust and expeditious protocols for genome editing of human Tregs are limited. Here, we describe a rapid and effective protocol for reaching high genome editing efficiencies in human Tregs without compromising cell integrity, suitable for potential therapeutic applications. By deletion of IL2RA encoding for IL-2 receptor α-chain (CD25) in Tregs, we demonstrated the applicability of the method for downstream functional assays and highlighted the importance for CD25 for in vitro suppressive function of human Tregs. Moreover, deletion of IL6RA (CD126) in human Tregs elicits cytokine unresponsiveness and thus may prevent IL-6-mediated instability of Tregs, making it an attractive target to potentially boost functionality in settings of adoptive Treg therapies to contain overreaching inflammation or autoimmunity. Thus, our rapid and efficient protocol for genome editing in human Tregs may advance possibilities for Treg-based cellular therapies.

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