Immunohistochemical study of epidermal Langerhans cells and dermal dendritic cells in benign and malignant skin lesions characterized by a dermal lymphoid infiltrate consisting either of B-cells or T-cells

1987 ◽  
Vol 411 (4) ◽  
pp. 337-343 ◽  
Author(s):  
M. Drijkoningen ◽  
C. Wolf-Peeters ◽  
J. Snauwaert ◽  
H. Greef ◽  
V. Desmet
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
B Cells ◽  
Langerhans Cells ◽  
Immunohistochemical Study ◽  
Skin Lesions ◽  
Lymphoid Infiltrate ◽  
Malignant Skin ◽  
Epidermal Langerhans Cells

Epidermal Langerhans' Cells and Dermal Dendritic Cells in Human Fetal and Neonatal Skin: An Immunohistochemical Study

1987 ◽  
Vol 4 (1) ◽  
pp. 11-17 ◽  
Author(s):  
M. Drijkoningen ◽  
C. Wolf-Peeters ◽  
K. Steen ◽  
P. Moerman ◽  
V. Desmet
Keyword(s):  
Dendritic Cells ◽  
Langerhans Cells ◽  
Immunohistochemical Study ◽  
Neonatal Skin ◽  
Epidermal Langerhans Cells

scholarly journals The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency

2011 ◽  
Vol 208 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Venetia Bigley ◽  
Muzlifah Haniffa ◽  
Sergei Doulatov ◽  
Xiao-Nong Wang ◽  
Rachel Dickinson ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cells ◽  
Dendritic Cells ◽  
Dendritic Cell ◽  
Peripheral Blood ◽  
Langerhans Cells ◽  
Elevated Serum ◽  
Blood Compartment ◽  
Hla Dr ◽  
Epidermal Langerhans Cells

Congenital or acquired cellular deficiencies in humans have the potential to reveal much about normal hematopoiesis and immune function. We show that a recently described syndrome of monocytopenia, B and NK lymphoid deficiency additionally includes the near absence of dendritic cells. Four subjects showed severe depletion of the peripheral blood HLA-DR+ lineage− compartment, with virtually no CD123+ or CD11c+ dendritic cells (DCs) and very few CD14+ or CD16+ monocytes. The only remaining HLA-DR+ lineage− cells were circulating CD34+ progenitor cells. Dermal CD14+ and CD1a+ DC were also absent, consistent with their dependence on blood-derived precursors. In contrast, epidermal Langerhans cells and tissue macrophages were largely preserved. Combined loss of peripheral DCs, monocytes, and B and NK lymphocytes was mirrored in the bone marrow by complete absence of multilymphoid progenitors and depletion of granulocyte-macrophage progenitors. Depletion of the HLA-DR+ peripheral blood compartment was associated with elevated serum fms-like tyrosine kinase ligand and reduced circulating CD4+CD25hiFoxP3+ T cells, supporting a role for DC in T reg cell homeostasis.

Murine epidermal Langerhans cells and splenic dendritic cells present tumor-associated antigens to primed T cells

1994 ◽  
Vol 24 (2) ◽  
pp. 315-319 ◽  
Author(s):  
Philip J. Cohen ◽  
Peter A. Cohen ◽  
Steven A. Rosenberg ◽  
James J. Mulé ◽  
Stephen I. Katz
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Langerhans Cells ◽  
Tumor Associated Antigens ◽  
Epidermal Langerhans Cells

In vitro primary sensitization of hapten-specific T cells by cultured human epidermal Langerhans cells - a screening predictive assay for contact sensitizers

1996 ◽  
Vol 26 (5) ◽  
pp. 563-570 ◽  
Author(s):  
M. KRASTEVA ◽  
J. PEGUET-NAVARRO ◽  
C. MOULON ◽  
P. COURTELLEMONT ◽  
G. REDZINIAK ◽  
...  
Keyword(s):  
T Cells ◽  
Langerhans Cells ◽  
Primary Sensitization ◽  
Predictive Assay ◽  
Epidermal Langerhans Cells

scholarly journals Murine Langerin + dermal dendritic cells prime CD 8 + T cells while Langerhans cells induce cross‐tolerance

2014 ◽  
Vol 6 (12) ◽  
pp. 1638-1638 ◽  
Author(s):  
Vincent Flacher ◽  
Christoph H Tripp ◽  
David G Mairhofer ◽  
Ralph M Steinman ◽  
Patrizia Stoitzner ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Langerhans Cells ◽  
Cross Tolerance

scholarly journals P01.02 TLR-mediated suppression of the CCL22-CCR4 axis as a new target for tumor immunotherapy

2021 ◽  
Vol 9 (Suppl 1) ◽  
pp. A3.2-A4
Author(s):  
J Grün ◽  
I Piseddu ◽  
C Perleberg ◽  
N Röhrle ◽  
S Endres ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
B Cells ◽  
Regulatory T Cells ◽  
Type I ◽  
List Type ◽  
Gm Csf

BackgroundUnmethylated CpG-DNA is a potent ligand for the endosomal Toll-like-receptor-9, important for the immune activation to pathogen-associated molecules.1 CpG and other TLR-ligands show effective immunotherapeutic capacities in cancer treatment by inducing an antitumorigenic immunity.2 They are able to reduce tumor progression by reduction of intratumoral secretion of the immunoregulating chemokine CCL223 and subsequent recruitment of immunosuppressive regulatory T cells (Treg), which express CCR4 the only so far known receptor for CCL22.4 Our recent work has shown that CCL22 secretion by dendritic cells (DC) in the lymph node, mediates tolerance by inducing DC-Treg contacts.5 Indeed, in the absence of CCL22, immune responses to vaccination were stronger and resulted in tumor rejection.6 Therefore, we are aiming to investigate the effects of TLR-ligands on systemic CCL22 levels, elucidating all involved mechanisms to identify new targets for cancer immunotherapy.Materials and MethodsT, B and CD11c+ DCs of wildtype (wt) and RAG1-/- mice were isolated from splenocytes by magnetic-activated cell sorting for in vitro assays. Different co-cultures were incubated with CpG and GM-CSF, known as an CCL22 inducer.5 For in vivo experiments, wt mice were treated with CpG, R484 or poly(I:C) alone and in combination with GM-CSF. CCL22-levels in a number of organs were analyzed.ResultsAnalyzing the different immune cell compartments in vitro, we found that DCs in whole splenocytes secrete CCL22 during culture while DC cultured alone showed no CCL22 secretion. When treated with CpG, CCL22-levels were reduced in splenocytes, while it was induced in DC culture alone. The same results were seen when RAG splenocytes, that lack functional B and T cells, were cultured with CpG. CpG treated B cells were able to suppress CCL22 secretion by DC unlike T cells alone. Co-cultures of T and B cells treated with CpG, however, induced the strongest CCL22 suppression in DC. In vivo, we could show that all TLR ligands tested reduced CCL22 in a number of organs significantly. Furthermore, CpG showed the strongest suppression of CCL22 even in the presence of the CCL22 inducer GM-CSF.5ConclusionsWe could show that B cells with T cells mediate CCL22 suppression by TLR ligands. The fact that CpG was able to reduce CCL22 levels even in the presence of the inducer GM-CSF demonstrates the potent CCL22 suppressive capacity of TLR ligands.ReferencesO’Neill LA, et al. The history of toll-like receptors – redefining innate immunity. Nat Rev Immunol 2013;13(6):453–60.Rothenfusser S, et al. Recent advances in immunostimulatory CpG oligonucleotides. Curr Opin Mol Ther 2003;5(2):98–106.Wang S, et al. Intratumoral injection of a CpG oligonucleotide reverts resistance to PD-1 blockade by expanding multifunctional CD8+ T cells. Proc Natl Acad Sci U S A 2016;113(46): E7240–E7249.Rapp M, et al. CCL22 controls immunity by promoting regulatory T cell communication with dendritic cells in lymph nodes. J Exp Med 2019;216(5):1170–1181.Piseddu I, et al. Constitutive expression of CCL22 is mediated by T cell-derived GM-CSF. J Immunol 2020;205(8):2056–2065.Anz D, et al. Suppression of intratumoral CCL22 by type i interferon inhibits migration of regulatory T cells and blocks cancer progression. Cancer Res 2015;75(21):4483–93.Disclosure InformationJ. Grün: None. I. Piseddu: None. C. Perleberg: None. N. Röhrle: None. S. Endres: None. D. Anz: None.

scholarly journals Expanded circulating follicular dendritic cells facilitate immune responses in chronic HBV infection

2020 ◽  
Author(s):  
Xiaoyi Li ◽  
Qifan Zhang ◽  
Wanyue Zhang ◽  
Guofu Ye ◽  
Yanchen Ma ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
B Cells ◽  
Hepatitis B ◽  
Immune Responses ◽  
Cd4 T Cells ◽  
Hbv Infection ◽  
Follicular Dendritic Cells ◽  
Chronic Hbv Infection ◽  
Chronic Hbv

Abstract Background: The restoration of host hepatitis B virus (HBV)-specific antiviral immunity is an effective strategy for hepatitis B recovery. Follicular dendritic cells (FDCs) play a crucial role in immune regulation. The goal of the present study was to investigate the characteristics and functions of FDCs in chronic HBV infection. Methods: The frequencies of FDCs in peripheral blood, liver, and spleen were measured in patients with chronic HBV infection. Isolated FDCs from splenic tissues of HBV-related liver cirrhosis-induced hypersplenism patients were cultured with autologous intrasplenic CD4 + T cells and CD19 + B cells.Results: We found that patients with chronic HBV infection had a significantly increased frequency of circulating FDCs compared with that of healthy controls. Additionally, the frequency of circulating FDCs was positively correlated with that of intrahepatic and intrasplenic counterparts. Moreover, a positive correlation between the frequency of circulating FDCs and plasmablast and memory B cells, as well as C-X-C motif chemokine receptor type 5 (CXCR5) + CD4 + T cells and CXCR5 + CD8 + T cells was also observed. Notably, in vitro experiments demonstrated that FDCs derived from splenic tissues of chronic HBV patients facilitated interferon-γ and interleukin-21 production from autologous intrasplenic CD4 + T cells and promoted the proliferation of autologous intrasplenic CD19 + B cells. Conclusions: Expanded FDCs in patients with chronic HBV infection may favor the host immune responses against HBV. The identification of this unique population may contribute to a better understanding of the immune regulatory mechanisms and provide a potential immunotherapeutic target in chronic HBV infection.

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