Faculty Opinions recommendation of Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation.

2015 ◽  
Author(s):  
Xiaojing Ma
Keyword(s):  
Gene Activation ◽  
Interferon Regulatory Factor ◽  
Toll Like Receptor ◽  
Regulatory Factor ◽  
Toll Like Receptor 4 ◽  
Interferon Regulatory Factor 3

scholarly journals The Rab11a GTPase Controls Toll-like Receptor 4-Induced Activation of Interferon Regulatory Factor-3 on Phagosomes

Immunity ◽  
2010 ◽  
Vol 33 (4) ◽  
pp. 583-596 ◽  
Author(s):  
Harald Husebye ◽  
Marie Hjelmseth Aune ◽  
Jørgen Stenvik ◽  
Eivind Samstad ◽  
Frode Skjeldal ◽  
...  
Keyword(s):  
Interferon Regulatory Factor ◽  
Toll Like Receptor ◽  
Regulatory Factor ◽  
Toll Like Receptor 4 ◽  
Interferon Regulatory Factor 3

Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation

Blood ◽  
2006 ◽  
Vol 107 (3) ◽  
pp. 1078-1084 ◽  
Author(s):  
Stanislas Goriely ◽  
Céline Molle ◽  
Muriel Nguyen ◽  
Valentina Albarani ◽  
Najate Ouled Haddou ◽  
...  
Keyword(s):  
Gene Activation ◽  
Ectopic Expression ◽  
Gene Promoter ◽  
Interferon Regulatory Factor ◽  
Raw 264.7 Cells ◽  
Interleukin 12 ◽  
Chain Gene ◽  
Toll Like Receptor ◽  
Regulatory Factor ◽  
Interferon Regulatory Factor 3

AbstractInterleukin-12 (IL-12) is a heterodimeric cytokine produced by dendritic cells (DCs) in response to Toll-like receptor (TLR) ligation. While the mechanisms regulating IL-12p40 chain gene expression are well characterized, molecular events involved in IL-12p35 chain gene activation remain to be clarified. Since IL-12p35 mRNA was induced in human DCs activated through TLR3 or TLR4 but not TLR2, we investigated the potential role of interferon regulatory factor 3 (IRF-3) in IL-12p35 gene transactivation. First, a binding site for IRF-3 named interferon-stimulated response element-1 (ISRE-1) was identified in the human IL-12p35 promoter region between nucleotides -251 and -240. The ISRE-1 site was required for IL-12p35 gene activation in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) or PolyI:C. Ectopic expression of IRF-3 was found to up-regulate IL-12p35 gene activation in the same system. Furthermore, chromatin immunoprecipitation (ChIP) studies demonstrated that IRF-3 is recruited to ISRE-1 site in TLR4- or TLR3-stimulated human DCs. Finally, experiments on DCs from IRF-3-deficient mice established that TLR4-induced IL-12p35 mRNA and IL-12p70 synthesis are impaired in absence of IRF-3. We conclude that IRF-3 binds to a critical cis-acting element in the IL-12p35 gene promoter and thereby represents a key factor for the induction of IL-12p70 synthesis in DCs.

Sign in / Sign up

Export Citation Format

Share Document