Faculty Opinions recommendation of sRNA-Mediated Control of Transcription Termination in E. coli.

2016 ◽  
Author(s):  
Luciano Marraffini ◽  
Jon McGinn
Keyword(s):  
Transcription Termination ◽  
E Coli

THE ROLE OF RHO IN TRANSCRIPTION TERMINATION IN E. COLI

1978 ◽  
pp. 163-169
Author(s):  
Max Gottesman ◽  
Sankar Adhya ◽  
Don Court ◽  
Asis Das
Keyword(s):  
Transcription Termination ◽  
E Coli

scholarly journals The Rho-Dependent Transcription Termination Is Involved in Broad-Spectrum Antibiotic Susceptibility in Escherichia coli

2020 ◽  
Vol 11 ◽  
Author(s):  
Md. Hafeezunnisa ◽  
Ranjan Sen
Keyword(s):  
Broad Spectrum ◽  
Antibiotic Susceptibility ◽  
Transcription Termination ◽  
Efflux Pumps ◽  
Dependent Manner ◽  
Broad Spectrum Antibiotic ◽  
E Coli ◽  
Distinct Cell ◽  
High Level ◽  
Termination Function

One of the major ways of acquiring multidrug resistance in bacteria is via drug influx and efflux pathways. Here, we show that E. coli with compromised Rho-dependent transcription termination function has enhanced broad-spectrum antibiotic susceptibility, which arises from the inefficient TolC-efflux process and increased permeability of the membrane. The Rho mutants have altered morphology, distinct cell surface, and increased levels of lipopolysaccharide in their outer membrane, which might have rendered the TolC efflux pumps inefficient. These alterations are due to the upregulations of poly-N-acetyl-glucosamine and lipopolysaccharide synthesis operons because of inefficient Rho functions. The Rho mutants are capable of growing on various dipeptides and carbohydrate sources, unlike their WT counterpart. Dipeptides uptake arises from the upregulations of the di-peptide permease operon in these mutants. The metabolomics of the Rho mutants revealed the presence of a high level of novel metabolites. Accumulation of these metabolites in these Rho mutants might titrate out the TolC-efflux pumps, which could further reduce their efficiency. We conclude that the transcription termination factor, Rho, regulates the broad-spectrum antibiotic susceptibility of E. coli through multipartite pathways in a TolC-dependent manner. The involvement of Rho-dependent termination in multiple pathways and its association with antibiotic susceptibility should make Rho-inhibitors useful in the anti-bacterial treatment regimen.

Fast Translation within the First 45 Codons Decreases mRNA Stability and Increases Premature Transcription Termination in E. coli

2019 ◽  
Vol 431 (6) ◽  
pp. 1088-1097 ◽  
Author(s):  
Steen Pedersen ◽  
Thilde Bagger Terkelsen ◽  
Mette Eriksen ◽  
Magnus Krarup Hauge ◽  
Casper Carstens Lund ◽  
...  
Keyword(s):  
Mrna Stability ◽  
Transcription Termination ◽  
E Coli

scholarly journals Expression of Different-Size Transcripts from theclpP-clpX Operon of Escherichia coli during Carbon Deprivation

2000 ◽  
Vol 182 (23) ◽  
pp. 6630-6637 ◽  
Author(s):  
Chin Li ◽  
Yi Ping Tao ◽  
Lee D. Simon
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Type Strain ◽  
Wild Type Strain ◽  
Transcription Termination ◽  
Carbon Starvation ◽  
Lower Percentage ◽  
Wild Type ◽  
Lower Efficiency ◽  
E Coli

ABSTRACT Transcription of the clpP-clpX operon ofEscherichia coli leads to the production of two different sizes of transcripts. In log phase, the level of the longer transcript is higher than the level of the shorter transcript. Soon after the onset of carbon starvation, the level of the shorter transcript increases significantly, and the level of the longer transcript decreases. The longer transcript consists of the entireclpP-clpX operon, whereas the shorter transcript contains the entire clpP gene but none of the clpXcoding sequence. The RpoH protein is required for the increase in the level of the shorter transcript during carbon starvation. Primer extension experiments suggest that there is increased usage of the ς32-dependent promoter of the clpP-clpXoperon within 15 min after the start of carbon starvation. Expression of the clpP-clpX operon from the promoters upstream of theclpP gene decreases to a very low level by 20 min after the onset of carbon starvation. Various pieces of evidence suggest, though they do not conclusively prove, that production of the shorter transcript may involve premature termination of the longer transcript. The half-life of the shorter transcript is much less than that of the longer transcript during carbon starvation. E. coli rpoBmutations that affect transcription termination efficiency alter the ratio of the shorter clpP-clpX transcript to the longer transcript. The E. coli rpoB3595 mutant, with an RNA polymerase that terminates transcription with lower efficiency than the wild type, accumulates a lower percentage of the shorter transcript during carbon starvation than does the isogenic wild-type strain. In contrast, the rpoB8 mutant, with an RNA polymerase that terminates transcription with higher efficiency than the wild type, produces a higher percentage of the shorter clpP-clpXtranscript when E. coli is in log phase. These and other data are consistent with the hypothesis that the shorter transcript results from premature transcription termination during production of the longer transcript.

Evidence against transcription termination within the E. coli lac operon

1974 ◽  
Vol 133 (4) ◽  
pp. 367-371 ◽  
Author(s):  
Louis W. Lim ◽  
David Kennell
Keyword(s):  
Transcription Termination ◽  
Lac Operon ◽  
E Coli

Requirement for E. coli NusG protein in factor-dependent transcription termination

Cell ◽  
1992 ◽  
Vol 68 (5) ◽  
pp. 989-994 ◽  
Author(s):  
Susan L. Sullivan ◽  
Max E. Gottesman
Keyword(s):  
Transcription Termination ◽  
E Coli

scholarly journals Transcription termination and anti-termination in E. coli

2002 ◽  
Vol 7 (8) ◽  
pp. 755-768 ◽  
Author(s):  
Evgeny Nudler ◽  
Max E. Gottesman
Keyword(s):  
Transcription Termination ◽  
E Coli

scholarly journals Bacteriophage P4 sut1: a mutation suppressing transcription termination

2007 ◽  
Vol 88 (3) ◽  
pp. 1041-1047
Author(s):  
Susanna Terzano ◽  
Ilaria Oliva ◽  
Francesca Forti ◽  
Claudia Sala ◽  
Francesca Magnoni ◽  
...  
Keyword(s):  
Stop Codon ◽  
Transcription Termination ◽  
Spontaneous Mutation ◽  
Wild Type ◽  
Stem Loop ◽  
Reading Frame ◽  
E Coli ◽  
Stem Loop Structure ◽  
Bacteriophage P4 ◽  
Sense Codon

In the Escherichia coli satellite phage P4, transcription starting from PLE is prevalently controlled via premature termination at several termination sites. We identified a spontaneous mutation, P4 sut1 (suppression of termination), in the natural stop codon of P4 orf151 that, by elongating translation, suppresses transcription termination at the downstream t151 site. Both the translational and the transcriptional profile of P4 sut1 differed from those of P4 wild-type. First of all, P4 sut1 did not express Orf151, but a higher molecular mass protein, compatible with the 303 codon open reading frame generated by the fusion of orf151, cnr and the intervening 138 nt. Moreover, after infection of E. coli, the mutant expressed a very low amount of the 1.3 and 1.7 kb transcripts originating at PLE and PLL promoters, respectively, and terminating at the intracistronic t151 site, whereas correspondingly higher amounts of the 4.1 and 4.5 kb RNAs arising from the same promoters and covering the entire operon were detected. Thus the sut1 mutation converts a natural stop codon into a sense codon, suppresses a natural intracistronic termination site and leads to overexpression of the downstream cnr and α genes. This correlates with the inability of P4 sut1 to propagate in the plasmid state. By cloning different P4 DNA fragments, we mapped the t151 transcription termination site within the 7633–7361 region between orf151 and gene cnr. A potential stem–loop structure, resembling the structure of a Rho-independent termination site, was predicted by mfold sequence analysis at 7414–7385.

Sign in / Sign up

Export Citation Format

Share Document