Faculty Opinions recommendation of Characterization of 2-Oxindole Forming Heme Enzyme MarE, Expanding the Functional Diversity of the Tryptophan Dioxygenase Superfamily.

Author(s):  
Gong-Li Tang
2017 ◽  
Vol 139 (34) ◽  
pp. 11887-11894 ◽  
Author(s):  
Yuyang Zhang ◽  
Yi Zou ◽  
Nelson L. Brock ◽  
Tingting Huang ◽  
Yingxia Lan ◽  
...  

1985 ◽  
Vol 260 (10) ◽  
pp. 6080-6087 ◽  
Author(s):  
L A Andersson ◽  
V Renganathan ◽  
A A Chiu ◽  
T M Loehr ◽  
M H Gold

2020 ◽  
Vol 118 (3) ◽  
pp. 119a-120a
Author(s):  
Irene Pertici ◽  
Giulio Bianchi ◽  
Lorenzo Bongini ◽  
Vincenzo Lombardi ◽  
Pasquale Bianco

2008 ◽  
Vol 52 (7) ◽  
pp. 2473-2479 ◽  
Author(s):  
Magdalena Stoczko ◽  
Jean-Marie Frère ◽  
Gian Maria Rossolini ◽  
Jean-Denis Docquier

ABSTRACT Metallo-β-lactamases (MBLs) are zinc-dependent bacterial enzymes characterized by an efficient hydrolysis of carbapenems and a lack of sensitivity to commercially available β-lactamase inactivators. Apart from the acquired subclass B1 enzymes, which exhibit increasing clinical importance and whose evolutionary origin remains unclear, most MBLs are encoded by resident genes found in the genomes of organisms belonging to at least three distinct phyla. Using genome database mining, we identified an open reading frame (ORF) (ECA2849) encoding an MBL-like protein in the sequenced genome of Erwinia carotovora, an important plant pathogen. Although no detectable β-lactamase activity could be found in E. carotovora, a recombinant Escherichia coli strain in which the ECA2849 ORF was cloned showed decreased susceptibility to several β-lactams, while carbapenem MICs were surprisingly poorly affected. The enzyme, named CAR-1, was purified by means of ion-exchange chromatography steps, and its characterization revealed unique structural and functional features. This new MBL was able to efficiently hydrolyze cephalothin, cefuroxime, and cefotaxime and, to a lesser extent, penicillins and the other cephalosporins but only poorly hydrolyzed meropenem, while imipenem was not recognized. CAR-1 is the first example of a functional naturally occurring MBL in the family Enterobacteriaceae (order Enterobacteriales) and highlights the extraordinary structural and functional diversity exhibited by MBLs.


2016 ◽  
Vol 138 (49) ◽  
pp. 16016-16023 ◽  
Author(s):  
Timothy H. Yosca ◽  
Matthew C. Langston ◽  
Courtney M. Krest ◽  
Elizabeth L. Onderko ◽  
Tyler L. Grove ◽  
...  

2020 ◽  
Author(s):  
Beatriz P. Cazorla ◽  
Javier Cabello ◽  
Andrés Reyes ◽  
Emilio Guirado ◽  
Julio Peñas ◽  
...  

Abstract. Conservation Biology faces the challenge of safeguarding the ecological processes that sustain biodiversity. Characterization and evaluation of these processes can be carried out through attributes or functional traits related to the exchanges of matter and energy between vegetation and the atmosphere. Nowadays, the use of satellite imagery provides useful methods to produce a spatially continuous characterization of ecosystem functioning and processes at regional scales. Our dataset characterizes the patterns of ecosystem functioning in Sierra Nevada (Spain) from the vegetation greenness dynamics captured through the spectral vegetation index EVI (Enhanced Vegetation Index) since 2001 to 2018 (product MOD13Q1.006 from MODIS sensor). First, we provided three Ecosystem Functional Attributes (EFAs) (i.e., descriptors of annual primary production, seasonality, and phenology of carbon gains), as well as their integration into a synthetic mapping of Ecosystem Functional Types (EFTs). Second, we provided two measures of functional diversity: EFT richness and EFT rarity. Finally, in addition to the yearly maps, we calculated interannual summaries, i.e., means and inter-annual variabilities. Examples of research and management applications of these data sets are also included to highlight the value of EFAs and EFTs to improve the understanding and monitoring ecosystem processes across environmental gradients. The datasets are available in two open-source sites (PANGAEA: https://doi.pangaea.de/10.1594/PANGAEA.904575 (Cazorla et al. 2019) and http://obsnev.es/apps/efts_SN.html), and bring to scientists, managers and the general public valuable information on the first characterization of the functional diversity at ecosystem level developed in a Mediterranean hotspot. Sierra Nevada represents an exceptional ecology laboratory of field conditions, where a long-term monitoring (LTER) program was established 10 years ago. The data availability on biodiversity, climate, ecosystem services, hydrology, land-use changes and management practices from Sierra Nevada, will allow to explore the relationships between these other environmental data and ecosystem functional data that we provide in this work.


2012 ◽  
Vol 196 (3) ◽  
pp. 726-737 ◽  
Author(s):  
Abhijit A. Karve ◽  
Sara S. Jawdy ◽  
Lee E. Gunter ◽  
Sara M. Allen ◽  
Xiaohan Yang ◽  
...  

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