Faculty Opinions recommendation of Soxb1 activity regulates sensory neuron regeneration, maintenance, and function in planarians.

The generation of sensory and autonomic neurons from the neural crest requires the functions of two classes of basic helix-loop-helix (bHLH) transcription factors, the Neurogenins (NGNs) and MASH-1, respectively (Fode, C., Gradwohl, G., Morin, X., Dierich, A., LeMeur, M., Goridis, C. and Guillemot, F. (1998) Neuron 20, 483–494; Guillemot, F., Lo, L.-C., Johnson, J. E., Auerbach, A., Anderson, D. J. and Joyner, A. L. (1993) Cell 75, 463–476; Ma, Q., Chen, Z. F., Barrantes, I. B., de la Pompa, J. L. and Anderson, D. J. (1998 Neuron 20, 469–482). We have cloned two chick NGNs and found that they are expressed in a subset of neural crest cells early in their migration. Ectopic expression of the NGNs in vivo biases migrating neural crest cells to localize in the sensory ganglia, and induces the expression of sensory neuron-appropriate markers in non-sensory crest derivatives. Surprisingly, the NGNs can also induce the expression of multiple pan-neuronal and sensory-specific markers in the dermomyotome, a mesodermal derivative. Taken together, these data suggest that a subset of neural crest cells may already be specified for a sensory neuron fate early in migration, as a consequence of NGN expression.

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Plasticity of connections of the olfactory sensory neuron: Regeneration into the forebrain following bulbectomy in the neonatal mouse

Neuroscience ◽

10.1016/0306-4522(79)90002-2 ◽

1979 ◽

Vol 4 (6) ◽

pp. 713-727 ◽

Cited By ~ 118

Author(s):

P.P.C. Graziadei ◽

R.R. Levine ◽

Giuseppina A. Monti Graziadei

Keyword(s):

Sensory Neuron ◽

Olfactory Sensory Neuron ◽

Neonatal Mouse ◽

Neuron Regeneration

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A video protocol for rapid dissection of mouse dorsal root ganglia from defined spinal levels

10.21203/rs.3.rs-25927/v1 ◽

2020 ◽

Cited By ~ 1

Author(s):

James Nicholas Sleigh ◽

Steven J West ◽

Giampietro Schiavo

Keyword(s):

Sensory Neuron ◽

Dorsal Root ◽

Genetic Diseases ◽

Spinal Column ◽

Spinal Cord Dorsal Horn ◽

Neuron Development ◽

Fine Detail ◽

Spinal Cord Dorsal ◽

And Function ◽

External Stimuli

Abstract Objective Dorsal root ganglion (DRG) are heterogeneous assemblies of assorted sensory neuron cell bodies found in bilateral pairs at every level of the spinal column. Unipolar afferent neurons convert external stimuli from the environment into electrical signals that are retrogradely transmitted to the spinal cord dorsal horn. To do this, they extend single axons from their DRG-resident somas that then bifurcate and project both centrally and distally. DRG can be dissected from mice at embryonic stages and any age post-natally, and have been extensively used to study sensory neuron development and function, response to injury, and pathological processes in acquired and genetic diseases. We have previously published a step-by-step dissection method for the rapid isolation of post-natal mouse DRG. Here, the objective is to extend the protocol by providing training videos that showcase the dissection in fine detail and permit the extraction of ganglia from defined spinal levels. Results By following this method, the reader will be able to swiftly and accurately isolate specific lumbar, thoracic, and cervical DRG from mice. Dissected ganglia can then be used for RNA/protein analyses, subjected to immunohistochemical examination, and cultured as explants or dissociated primary neurons, for in-depth investigations of sensory neuron biology.

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Role of Histamine as a Peripheral Sympathetic Neuromediator and its Interrelation with Substance P

Current Pharmaceutical Design ◽

10.2174/1381612826666200813132951 ◽

2020 ◽

Vol 26 (35) ◽

pp. 4486-4495

Author(s):

Augusto S. Manzo Atencio ◽

Flor A. Perez de Manzo ◽

Manuel Velasco

Keyword(s):

Nervous System ◽

Substance P ◽

Sensory Neuron ◽

And Function

This article is an educational review about the fundamental aspects related to the proposal of the existence of a peripheral sympathetic reflex regulated by histamine, through its effect on presynaptic H3 type receptors, under the interaction of a sensory neuron that would be mediated by Substance P. In this respect, we consider it useful to highlight the role of histamine, so we discuss some aspects about its history, metabolism, and function, as well as its interaction with H3 type receptors that are considered as neuroreceptors, which define and typify it as a neuromediator at both levels of the nervous system, central and peripheral.

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