<p>The activation-tagged populations of transgenic rice cv.<br />Asemandi have been developed by introducing construct of<br />activation taq Ac/Ds into rice genom of cv. Asemandi. The T1<br />transgenic rice populations cv. Asemandi containing<br />construct of activation taq have been obtained and needed<br />to be characterized. This study aimed to identify the Basta<br />herbicide resistant plants, transgenic plants containing hpt<br />and bar genes, and changes in agronomic traits. Basta<br />resistant plant was identified by treating leaf with basta<br />solution. Hpt and bar genes were detected by PCR using<br />specific primers. Phenotype characters were identified by<br />observing and measuring their agronomic parameters. The<br />study results showed that out of 315 rice transgenic cv.<br />Asemandi T1 treated with Basta solution, 176 (55.87%) plants<br />were indicated to be resistant to Basta. The results of PCR<br />analysis revealed that eight rice transgenic cv. Asemandi<br />tested contained both hpt and bar genes. In general,<br />compared to the nontransgenic plants, there were changes<br />in several agronomic parameters of T1 transgenic plants cv.<br />Asemandi, including plant height, days to flowering, days to<br />harvesting, periods of grain filling, and weight of 100 grains.<br />Correlation analysis showed that there was no correlation<br />between days of harvesting to weight of 100 grains in<br />transgenic rice, but there was correlation in nontransgenic<br />rice. Transgenic rice plants cv. Asemandi with changes in<br />the agronomic characters will be useful for further study,<br />such as to analyze the function of the genes.</p>
Studying the relationships of various agronomic traits in maize using correlation analysis