scholarly journals Detection of two Shiga-like toxins from Escherichia coli O157:H7 isolates by the polymerase chain reaction method.

1990 ◽  
Vol 45 (3) ◽  
pp. 649-652 ◽  
Author(s):  
Kazuhiro KOBAYASHI ◽  
Kazuko SETO ◽  
Masanao MAKINO
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Escherichia Coli O157 ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Establishment of a Polymerase Chain Reaction Method for Detection of Escherichia coli in Amniotic Fluid in Patients with Chorioamnionitis

2008 ◽  
Vol 24 (2) ◽  
pp. 132-139 ◽  
Author(s):  
Ghada A. Daoud ◽  
Yoshikatsu Suzuki ◽  
Tamao Yamamoto ◽  
Takashi Suzuki ◽  
Nobuhiro Suzumori ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Amniotic Fluid ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Development and application of a real-time polymerase chain reaction method for quantification of Escherichia coli in oysters (Crassostrea gigas)

2019 ◽  
Vol 77 ◽  
pp. 85-92 ◽  
Author(s):  
Marília Miotto ◽  
Antônio A. Fonseca ◽  
Clarissa Barretta ◽  
Helen S. da Silva ◽  
Tomás Pellizzaro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Crassostrea Gigas ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Rapid and sensitive immunomagnetic separation–polymerase chain reaction method for the detection of Escherichia coli O157[ratio ]H7 in raw milk and ice-cream

1997 ◽  
Vol 64 (1) ◽  
pp. 87-93 ◽  
Author(s):  
CHRISTOPHER M. GOODING ◽  
PRABHAKARA V. CHOUDARY
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Ice Cream ◽  
Raw Milk ◽  
Immunomagnetic Separation ◽  
Polymerase Chain Reaction Method ◽  
Escherichia Coli O157 ◽  
Agarose Gel Electrophoresis ◽  
Chain Reaction ◽  
Polymerase Chain

Escherichia coli O157[ratio ]H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157[ratio ]H7/g food could be detected in <10 h.

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