scholarly journals Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

2019 ◽  
pp. 69-79
Author(s):  
Faria Farhana Rain ◽  
Abdul Jabber Howlader ◽  
Abu Faiz Md. Aslam
Keyword(s):  
Research Work ◽  
Dna Barcode ◽  
Haplotype Network ◽  
First Record ◽  
Coi Gene ◽  
Gene Sequences ◽  
Accurate Identification ◽  
Haematobia Irritans ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Flies are considered serious pests which cause health problems of human and animal, transmitting many pathogenic microbes. Pest management programs depend on proper identification of pests. The present research work is an initiative to identify the medically and veterinary important flies based on mitochondrial COI gene sequences. Eleven species of the fly pests were identified. Among them, four fly species were the first record from Bangladesh. The phylogenetic analysis of retrieved sequences confirmed that the evolution of these species occurred from a common ancestor. Highest AT percentage (69.9%) was found in Haematobia irritans exigua and lowest GC percentage (30.4%) was found in Haematobia irritans exigua. The substitution rate of codon was found 1.88 in 1st position, 0.73 in 2nd position and 1.22 in 3rd position, respectively. Interspecific genetic divergence range of flies sequences was 5-20%. Haplotype network showed that Atylotus agrestis was mostly diverged from its common ancestors by 37 mutational steps. This research is the first molecular approach to identify the medically and veterinary important flies based on MT-COI gene sequences along with the establishment of first DNA barcode dataset for accurate identification in Bangladesh.

scholarly journals First record of Zaprionus indianus (Gupta, 1970) (Diptera Drosophilidae) from Bangladesh

2011 ◽  
Vol 11 (3) ◽  
pp. 757-760
Author(s):  
Sadniman Rahman ◽  
Deponkor Kumar Roy ◽  
Mohammad Shamimul Alam
Keyword(s):  
Gene Sequence ◽  
First Record ◽  
Coi Gene ◽  
Zaprionus Indianus ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Zaprionus indianus (Gupta, 1970) (Diptera Drosophilidae), also known as African fig fly, isconsidered as the primary pest only to fig fruits along with secondary pest to other fruits. Its oc-currence has been reported from many countries but not previously reported from Bangladesh.With this note, we report here the first observation of Z. indianus from Bangladesh. Fly sampleswere collected by using yeast-banana traps. The species was identified by morphological char-acteristics and mitochondrial COI gene sequence.

scholarly journals Species detections in environmental DNA using metabarcoding: a useful tool in estuaries monitoring

2015 ◽  
Author(s):  
Adrián Mártinez-Marqués ◽  
Carlos Enrique Carleos ◽  
Eva García-Vazquez ◽  
Yaisel J. Borrell Pichs
Keyword(s):  
Dna Analysis ◽  
Anthropogenic Activities ◽  
Dna Barcode ◽  
Environmental Dna ◽  
Coi Gene ◽  
Universal Primers ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Cantabrian Coast ◽  
Taxonomic Groups

Estuaries are amongst the most productive habitats in Earth, producing more organic materia than forests, meadows or agricultural lands. In addition, estuaries exhibit high, and precious, biodiversity levels. In this study an environmental DNA analysis of the two most important estuaries in Asturias (Cantabrian Coast, north Iberia) in terms of food production (Ría del Eo and Ría de Villaviciosa) was carried out. The objective was to monitor aquatic biodiversity and also to detect alien species that can be associated with anthropogenic activities (e.g.: aquaculture). To achieve these objectives, a metabarcoding methodology based in NGS (next generation sequencing) and the mitochondrial COI gene as a DNA Barcode was used. Results showed that this methodology was useful to detect the presence of three different non-native genera (Crepidula, Lymnaea, Macrobrachium) that are probably parasitating species cultured in these estuaries. It is true that Metabarcoding has still unsolved problems such as the lack of 100% universal primers and paucity of referenced sequences for some taxonomic groups in the databases. However, it represents already a powerful tool to manage the resources of these important ecosystems and to guarantee their long-term sustainailibity.

Genetic Diversity and Population Structure of Panonychus citri (Acari: Tetranychidae), in China Based on Mitochondrial COI Gene Sequences

2010 ◽  
Vol 103 (6) ◽  
pp. 2204-2213 ◽  
Author(s):  
Ming-Long Yuan ◽  
Dan-Dan Wei ◽  
Kun Zhang ◽  
Yu-Zhen Gao ◽  
Yong-Hua Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Coi Gene ◽  
Gene Sequences ◽  
Panonychus Citri ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene
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