scholarly journals Method for purification of collagen: A systematic review

2020 ◽  
pp. 99-112
Author(s):  
Nursyahidatul Azwa Awang ◽  
Azura Amid ◽  
Zatul Iffah Arshad
Keyword(s):  
Systematic Review ◽  
High Yield ◽  
Market Demand ◽  
Purification Method ◽  
Affinity Tags ◽  
Advantages And Disadvantages ◽  
Qualitative Systematic Review ◽  
Purification Methods ◽  
Technical Details ◽  
Different Sources

Collagen has diverse applications in various industries and thus a various method of purifications has been developed over the years to produce high yield and high purity of collagen to fulfill collagen market demand. The objective of this qualitative systematic review is to summarize the current literature on the existing purification methods for collagen from different sources. Toward this end, three databases were searched and articles were screened for all original articles explaining the purification of collagen regardless of their main sources. After the comprehensive screening, out of 715 articles, 12 articles were chosen and were further reviewed. The analysis of the technical details, theory, advantages, and disadvantages of the techniques used are reported in this study. We found four types of purification methods that commonly used to purify various types of collagen from mammals, marine, bacteria, and recombinant collagen. The correlations between the prevalence of the method and the efficiency of collagen purification were also identified. It highlighted that many factors should be considered before choosing any method of purification such as types, sources and structure of the collagen itself. Affinity chromatography commonly used for purification of recombinant collagen as they have been genetically modified and appended by affinity tags whereas conventional purification method is preferable for non-recombinant collagen from mammalian and marine sources.

scholarly journals Preclinical extracranial aneurysm models for the study and treatment of brain aneurysms: A systematic review

2020 ◽  
Vol 40 (5) ◽  
pp. 922-938 ◽  
Author(s):  
Serge Marbacher ◽  
Fabio Strange ◽  
Juhana Frösén ◽  
Javier Fandino
Keyword(s):  
Systematic Review ◽  
Text Analysis ◽  
Experimental Models ◽  
Advantages And Disadvantages ◽  
Novel Treatment ◽  
Pubmed Database ◽  
Basic Understanding ◽  
Technical Modifications ◽  
Technical Details ◽  
Brain Aneurysms

Animal models make an important contribution to our basic understanding of the pathobiology of human brain aneurysms, are indispensable in testing novel treatment approaches, and are essential for training interventional neuroradiologists and neurosurgeons. Researchers are confronted with a broad diversity of models and techniques in various species. This systematic review aims to summarize and categorize extracranial aneurysm models and their characteristics, discuss advantages and disadvantages, and suggest the best use of each model. We searched the electronical Medline/PubMed database between 1950 and 2020 to identify main models and their refinements and technical modifications for creation of extracranial aneurysms. Each study included was assessed for aneurysm-specific characteristics, technical details of aneurysm creation, and histological findings. Among more than 4000 titles and abstracts screened, 473 studies underwent full-text analysis. From those, 68 different techniques/models in five different species were identified, analyzed in detail, and then grouped into one of the five main groups of experimental models as sidewall, terminal, stump, bifurcation, or complex aneurysm models. This systematic review provides a compact guide for investigators in selecting the most appropriate model from a range of techniques to best suit their experimental goals, practical considerations, and laboratory environment.

scholarly journals Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue

Cell Medicine ◽  
2018 ◽  
Vol 10 ◽  
pp. 215517901773309 ◽  
Author(s):  
Nana Ebi ◽  
Chika Miyagi-Shiohira ◽  
Eri Hamada ◽  
Yoshihito Tamaki ◽  
Mariko Masamoto ◽  
...  
Keyword(s):  
Density Gradient ◽  
Stimulation Index ◽  
High Yield ◽  
Islet Isolation ◽  
Pancreatic Islet Transplantation ◽  
Porcine Pancreas ◽  
Purification Method ◽  
Continuous Density ◽  
Yield Rate ◽  
Purification Methods

Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. One method involved loading digested tissue on top of a continuous density gradient (top loading). The other method involved mixing digested tissue with low-density solution and then making a continuous gradient (mixed loading). There were no significant differences between the 2 purification methods in terms of the islet yield, rate of viability or purity, score, or in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these 2 methods of islet purification.

Grieving the loss of a pet: A qualitative systematic review

Death Studies ◽  
2021 ◽  
pp. 1-12
Author(s):  
Michelle Cleary ◽  
Sancia West ◽  
Deependra K. Thapa ◽  
Mark Westman ◽  
Kristina Vesk ◽  
...  
Keyword(s):  
Systematic Review ◽  
Qualitative Systematic Review

scholarly journals Barriers to Incident Reporting among Nurses: A Qualitative Systematic Review

2021 ◽  
pp. 019394592199944
Author(s):  
Moataz Mohamed Maamoun Hamed ◽  
Stathis Konstantinidis
Keyword(s):  
Systematic Review ◽  
Methodological Quality ◽  
Incident Reporting ◽  
Negative Consequences ◽  
Incident Reporting Systems ◽  
Qualitative Systematic Review ◽  
Blame Culture ◽  
Critical Appraisal Skills ◽  
Definition Of

Incident reporting in health care prevents error recurrence, ultimately improving patient safety. A qualitative systematic review was conducted, aiming to identify barriers to incident reporting among nurses. Joanna Briggs Institute methodology for qualitative systematic reviews was followed, with data extracted using JBI QARI tools, and selected studies assessed for methodological quality using Critical Appraisal Skills Program (CASP). A meta-aggregation synthesis was carried out, and confidence in findings was assessed using GRADE ConQual. A total of 921 records were identified, but only five studies were included. The overall methodological quality of these studies was good and GRADE ConQual assessment score was “moderate.” Fear of negative consequences was the most cited barrier to nursing incident reporting. Barriers also included inadequate incident reporting systems and lack of interdisciplinary and interdepartmental cooperation. Lack of nurses’ necessary training made it more difficult to understand the importance of incident reporting and the definition of error. Lack of effective feedback and motivation and a pervasive blame culture were also identified.

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