scholarly journals IN VITRO BREEDING AND NURSING TAQUA BANANA IN TRA VINH PROVINCE

2019 ◽  
Vol 1 (26) ◽  
pp. 102-108
Author(s):  
Tam Thi Thanh Dinh ◽  
Nam Quoc Phan ◽  
Trai Ngoc Nguyen ◽  
An Van Vo ◽  
Phuong Minh Nguyen
Keyword(s):  
In Vitro Propagation ◽  
Ms Medium ◽  
Dark Condition ◽  
Bud Regeneration ◽  
Adenine Sulfate ◽  
Good For ◽  
Optimal Medium ◽  
Propagation Media

The goal of this study is to determine in vitro propagation media of Taqua banana. The results showed that: the optimal medium for banana bud regeneration was MS medium (Murashine & Skoog 1962) supplemented 0.1 mg/l NAA, 100 mg/l adenine sulfate, 30g/l sucrose, 8 g/l Agar, 5 mg/l BAP, 10% volume coconut juice and kept in completely dark condition. The medium, which is similar to bud generation media except for supplementing BAP 7 mg/l, was also good for bud replication with  6,33 shoots per sample after 4 weeks. (2)The medium, which is similar to bud generation media except for non-added BAP and an increase of NAA from 0.1 to 1 mg/l, was the best for the banana rooting.

scholarly journals KOMUNIKASI PENDEK Perbanyakan Tanaman Artemisia annua secara In Vitro

2016 ◽  
Vol 4 (1) ◽  
pp. 41
Author(s):  
Rossa Yunita ◽  
Endang G. Lestari
Keyword(s):  
Plasmodium Falciparum ◽  
Cerebral Malaria ◽  
In Vitro Propagation ◽  
Artemisia Annua ◽  
Ms Medium ◽  
Marked Activity ◽  
Good For

<p>Artemisinin, an anti-malarial medicine<br />isolated from the annual wormwood Artemisia annua,<br />has a marked activity against chloroquine-resistant and<br />chloroquine-sensitive strains of Plasmodium falciparum.<br />This compound is useful for treatment of cerebral malaria.<br />An in vitro propagation system for A. annua has been developed.<br />Shoots were induced by culturing seeds of A.<br />annua on a MS medium containing BAP (0, 0.1, 0.3, 0.5<br />mg/l). Shoots were also formed on each seedling cultured<br />on the same medium. Root formations were obtained from<br />shoots that were subcultured on a MS medium containing<br />IBA (0, 1.0, 1.5, 2 mg/l). The results showed that MS medium<br />supplemented with BAP 0.3 mg/l was the best medium for<br />induction and multiplication of the shoots, while the MS<br />medium supplemented with IBA (1 mg/l) was good for root<br />formations.</p>

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

scholarly journals Effect of fungicides on the growth of fungi isolated from in vitro propagated fruit-bearing plants

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 329-331
Author(s):  
M. Kowalik ◽  
M. Gródek
Keyword(s):  
In Vitro Propagation ◽  
Linear Growth ◽  
Linear Growth Rate ◽  
Ms Medium ◽  
Penicillium Verrucosum ◽  
Mortierella Isabellina ◽  
Cladosporium Sphaerospermum ◽  
Murashige Skoog ◽  
Growth Of Fungi

The aim of the work was to determine the effect of fungicides: Topsin M 70 WG, Bravo 500 SC, and Sportak 45 EC in two concentrations on the growth of fungi: Aspergillus niger, Epicoccum purpurascens, Cladosporium sphaerospermum, Mortierella isabellina and Penicillium verrucosum var. verrucosum on PDA and Murashige-Skoog medium (MS). The smallest index of linear growth rate of the fungi had Topsin M 70 WG for fungi: E. purpurascens and P. verrucosum var. verrucosum on PDA and MS medium and for M. isabellina and C. sphaerospermum on MS in concentrations of 71.42 mg/l and 107.13 mg/l. The highest index of linear growth had Bravo 500 SC in concentrations of 0.5 ml/l and 0.75 ml/l on PDA for E. purpurascens and A. niger. The lowest index of linear growth on MS for E. purpurascens, C. sphaerospermum and P. verrucosum var. verrucosum was noted down. The highest effectiveness in control of linear growth all tested species of fungi was found for fungicide Sportak 45 EC in concentration of 0.55 ml/l and 0.825 ml/l on MS and PDA. Among all tested fungicides used in vitro propagation fungicides from imidazoles group are recommended.

scholarly journals In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

2015 ◽  
Vol 41 ◽  
pp. 71-77
Author(s):  
S. Parvin ◽  
M. Kausar ◽  
M. Enamul Haque ◽  
M. Khalekuzzaman ◽  
B. Sikdar ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Ms Medium ◽  
Cotyledonary Nodes ◽  
Cucumis Melo L

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from invitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growthregulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.Effective result was found on MS medium supplemented with 2.0 mg/l BAP, inwhich 90% and 70% cultures induced multiple shoots from nodal segments andshoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodeswere found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS mediumsupplemented with different concentrations of IBA and NAA for successful rootinduction and the effective result (up to 70%) was found in medium with 1 mg/lIBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, whereas 70% plantlets survived

rol-Transgenic Populus tremula: root development, root-borne bud regeneration and in vitro propagation efficiency

Trees ◽  
1998 ◽  
Vol 12 (8) ◽  
pp. 464-471 ◽  
Author(s):  
Tzvi Tzfira ◽  
Basia Vinocur ◽  
A. Altman ◽  
Alexander Vainstein
Keyword(s):  
Root Development ◽  
In Vitro Propagation ◽  
Populus Tremula ◽  
Bud Regeneration

In vitro propagation, genetic and phytochemical assessment of Thymus persicus — a medicinally important source of pentacyclic triterpenoids

Biologia ◽  
2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Ziba Bakhtiar ◽  
Mohammad Mirjalili ◽  
Ali Sonboli ◽  
Mahdi Farimani ◽  
Mahdi Ayyari
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
In Vitro Propagation ◽  
Random Amplified Polymorphic Dna ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Pentacyclic Triterpenoids ◽  
Thymus Persicus

AbstractThymus persicus (Ronniger ex Rech. f.) Jalas is a valuable and endangered natural source of antitumor pentacyclic triterpenoids, i.e., betulinic acid, oleanolic acid and ursolic acid, which grows in northwest Iran. As the plant has a low propagation rate in nature, a suitable method for in vitro-propagation is needed. With the aim of identifying a suitable system for regenerating T. persicus via direct organogenesis, Murashige & Skoog (MS) medium supplemented with different plant growth regulators (PGRs) was tested. In vitro-grown shoot tips were exposed to the cytokinins 6-benzylaminopurine (BAP), kinetin (KN), and thidiazuron (TDZ), alone or in combination with the auxins 1-naphthalene-acetic acid (NAA), 2,4-dichlorophenoxyacetic (2,4-D), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The highest shoot formation (7.1 ± 0.9) was obtained with a medium fortified with 8.9 μM BAP plus 2.7 μM NAA. Regenerated shoots were easily rooted on the different tested media, with the most abundant (16.6 ± 1.4) and strongest roots obtained on half-strength MS medium containing 2.5 μM IBA. The rooted plantlets were successfully acclimatized (76.6%) in a greenhouse before transference to natural conditions. Homogeneity and phytochemical productivity of the in vitro regenerated plantlets were confirmed by random amplified polymorphic DNA (RAPD) profiles and high performance liquid chromatography (HPLC), respectively.

scholarly journals In Vitro Propagation of Agave americana by Indirect Organogenesis

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 996-999 ◽  
Author(s):  
Carlos Alberto Lecona-Guzmán ◽  
Sheila Reyes-Zambrano ◽  
Felipe Alonso Barredo-Pool ◽  
Miguel Abud-Archila ◽  
Joaquín Adolfo Montes-Molina ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Indirect Organogenesis ◽  
Meristematic Tissue ◽  
Phenotypic Alteration ◽  
Sexual And Asexual Reproduction ◽  
Agave Americana

Factors such as slow growth, low rates of sexual and asexual reproduction, and viability of seeds among others limit the massive propagation of Agave americana L. by conventional methods. In this study, callus induction and shoot proliferation was determined in A. americana using Murashige and Skoog (MS) medium supplemented with dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA). Meristematic tissue was used as the explants, and were placed on MS medium supplemented with 30.0 g·L−1 sucrose with 0.11, 0.18, or 0.45 μm 2,4-D and 11.0, 22.0, 38.2, 44.0, 58.7, or 73.3 μm BA. Treatments were implemented according to factorial experimental design 3 × 6. After 1 month, the number of explants with callus was determined, whereas the numbers of shoots per explant were monitored after 4, 16, 20, and 36 weeks. The maximum percent of explants with callus was obtained with 0.11 μm 2,4-D and 58.7 and 73.3 μm BA, whereas the maximum numbers of shoots per explant (71) were obtained with 0.11 μm 2,4-D and 73.3 μm BA. The effect of different concentrations of indolebutyric acid (IBA) in the rooting of shoots was evaluated. There were no significant effects of IBA on the number of roots, root length, and axillary roots. Plantlets were acclimatized in the glasshouse and they did not show any phenotypic alteration. This is a highly efficient protocol for the in vitro propagation of A. americana via indirect organogenesis.

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

scholarly journals 882 PB 276 IN VITRO PROPAGATION AND CHIMERAL TRAITS OF Cryptanthus `Marian Oppenheimer' (WIDE LEAF CLONE)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560c-560
Author(s):  
Yong Cheong Koh ◽  
Fred T. Davies
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Periclinal Chimera ◽  
Adventitious Shoot Formation ◽  
L1 And L2

The leaves of vegetative stolons of greenhouse grown Cryptanthus `Marian Oppenheimer' (wide leaf clone) were cultured in modified MS media to induce adventitious shoot formation via callus formation. The best callus induction medium was basal MS medium with 10 μM NAA, IBA and BA. Pure green (843), maroon (3), striped (2) and albino plantlets were obtained. Most of the albino plantlets were stunted, tightly clumped together and impossible to score. The medium which produced the highest average number of non-albino plantlets was basal MS medium with 0.3 μM NAA, IBA and BA All non-albino plantlets were rooted in MS medium with 5.4 μM NAA and transplanted ex vitro with a survival rate of 96.7%. The maroon plantlets became green two weeks after transplanting. Histological studies revealed that C. `Marian Oppenheimer' (wide leaf clone) has two tunicas (L1 and L2) and a corpus (L3). Callus on the leaf explant arose mainly from the L2 and L3. Apparently C. `Marian Oppenheimer' (wide leaf clone) is a GWG periclinal chimera.

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