Effects of Different Lighting Conditions on Growth, Yield and Nutrient Content of White Oyster Mushroom in Vertical Farm

The world population is continuously growing at a rapid rate, so a great challenge for this time is to ensure the security of food and nutrition for this increased population seeking for new crop as a source of food and nutrition. In such state mushrooms, a great source of protein, can be a favor that can be cultivated by landless people using agricultural waste material. The research work was about to analyze the effect of three lighting conditions namely dark condition (C1), medium lighting condition (C2), and sunlight (C3) on growth, yield, and nutrient content of pleurotus ostreatus (white oyster mushroom). Vertical farming technology was applied in mushroom production in order to minimize the land use. Three vertical structures each of three layers were used. This study also encompassed the effects of different layers (bottom later L1, middle layer L2, top layer L3) on the growth and yield of mushroom. The findings of this investigation revealed that there were significant differences in growth and yield at different conditions and structural layers. The highest average growth and yield (stalk height 3.87 cm, cap size 11.89 cm, stipe size 2.65 cm, crops per bag 15.78, fresh weight 60.03 g, and dry weight 17.03 g) was observed in dark condition (C1). On the other hand, lowest growth, and yield (stalk height 1.59 cm, cap size 3.67 cm, stipe size 1.51 cm, crops per bag 4.22, fresh weight 18.38 g, and dry weight 4.47 g) was found in sunlight (C3). Oyster mushroom cultivated in dark condition C1 also have high nutrient content. The study suggested that maintaining the absolute darkness is capable of producing high-quality white oyster mushroom.

Morphoanatomy of Pangasionodon hypopthalmus Reared Under Controlled Photoperiod

Fish morphoanatomy is affected by environmental condition such as photoperiod duration. A research on the effects of shortened photoperiod on the morphoanatomy of Pangasionodon hypopthalmus has been conducted. There were 3 treatments applied, namely, 24 hours dark (24D), 18 hours dark (18D) and natural photoperiod (control). The fish, 8 cm total length (TL) and 4 gr body weight (BW) were reared in circular plastic tanks filled with 100 L freshwater and completed with aerators and circulation pumps, 30 fishes/tank. The fish was reared for 8 weeks and fed with commercial fish feed pellets, twice/day, at satiation. The dark condition was created by placing the tanks under dark tarp tents. Morphoanatomy data were studied by the end of experiment. Results shown that the survival of fish was 100%. The growth of fish reared in 24D and 18D was better, more than 20 cm TL and 85 gr BW, while that of the control was 18 cm TL and 72 gr BW. The condition factor as well as the liver condition of fish in all treatment was not different, it was around 1.2. The liver was reddish brown color, smooth and oily in which hepatosomatic index (HSI) value 0.023 in 24D; 0.027 in 18D and 0.048 in control. The visceralsonatic index (VSI) was 0.090 in 24D; 0.70 in 18D and 0.161 in control. In most of fishes reared in 24D and 18D, the gonad were in the 1st maturity stage (gonadosomatic index; GSI 0.0058 to 0.0068), while the gonad of some control fishes were in the 2nd maturity stage (GSI 0.0080). Data obtained shown that the morphoanatomical data (HSI, VSI and GSI) of the fish reared in lengthened dark were lower, but the growth data (TL and BW) was higher than those of the control, indicated that the fish reared in dark condition may allocated more energy for growing.

Glucose Induces Thylakoid Formation and Upregulates Green Pigment Contents in Complete Dark Culture of the Angiosperm Pachiramacrocarpa

In addition to angiosperms, most plants are able to synthesize chlorophyll (Chl)-generating green tissues in total darkness. In this study, 140 plants of the angiosperm Pachira macrocarpa were divided into five groups. Among them, one group was grown for 2 weeks under natural light conditions, whereas the others were grown in complete darkness (0 μmol m−2 s−1). Dark-grown plants were then treated with 0~6% glucose for another 8 weeks. The budding and greening ratios, ultrastructure of chloroplasts (ChlPs) of newly developed leaves, and green pigment contents of pre-illuminated mature and young leaves, and totally dark-grown newly developed leaves were measured. Results showed that glucose inhibited the budding and promoted the greening of newly developed leaves. Pre-illuminated mature and young leaves were able to synthesize green pigments during the 2 weeks of dark adaption. Dark-grown newly developed leaves contained high levels of green pigments at 2 and 3 weeks after budding. Green pigments of glucose-fed newly developed leaves had increased, whereas they had decreased in control leaves. In addition, ChlPs of dark-grown glucose-fed newly developed leaves contained both giant grana and prolamellar bodies (PLBs), usually found in shade plants and etiolated seedlings, respectively. The higher the glucose concentration was, the greater the numbers of grana, thylakoids, and PLBs. Glucose increased the green pigment contents and grana formation in newly developed leaves in a dark condition, and the mechanisms are discussed.

Development of Photocatalytic 3D-Printed Cementitious Mortars: Influence of the Curing, Spraying Time Gaps and TiO2 Coating Rates

This work evaluated the photocatalytic activity of 3D-printed cementitious mortar specimens functionalized with TiO2 nanoparticles to obtain a multifunctional and smart concrete. This research aims to assess the influence of different parameters related to the functionalization process such as adsorption, coating time gaps, and coating rates on the degradation efficiency of the functionalized cementitious specimens. Each specimen was evaluated under the degradation of Rhodamine B (RhB) in an aqueous solution using a sun-light simulation. The obtained results showed a decrease in adsorption (under dark condition) with increasing the sample curing age. The highest photocatalytic efficiency was observed for coated samples aged 7 days. By increasing the coating rates, the photocatalytic efficiency is enhanced. Nonetheless, regardless of the coating rates, all the specimens showed an increase in photocatalytic efficiency for longer time periods of light exposition, i.e., after 8 h of irradiation.

Lampiran 4A Paper Degradasi paraquat (1,1'-dimetil-4,4'bipyridilium) dalam Lingkungan Tanah Desa Oematanunu Kecamatan Kupang Barat

In thus research, the kinetics of paraquat degradation in a medium of Oematnunu soil filtrate medium at two conditions, i. e. light condition and dark condition (on direct sunshine for 8 hours per day) has been studied. to study the effect of sunshine in paraquat degradation, it gas been carried out a paraquat degradation in medium of sterilized aquadest, sterilized well water, sterilized Oematnunu soil filtrate, medium without sterilization like, medium aquadest, medium well water and medium Oematnunu soil filtrate without sterilization. on certain time interval, the rest of paraquat was determined by UV-Vis spectrophotometry after being reduced with sodium dithionite at a maximum wavelength of 604 nm. the results indicated that sunshine increased the rate of paraquat degradation. paraquat degradation studied medium followed kinetics of the first order. the rate constant of paraquat in Oematnunu soil filtrate medium (0,06998 0,00336 /day). higher than that in medium without sterilization and anothers sterilization medium, as well as in well water medium (0,06217 0,00317 /day), aquadest medium (0,03458 0,00252 /day), for anothers sterilized medium as Oematnunu soil filtrate medium (0,06086 0,00285 /day), sterilized well water medium (0,04720 0,00182 /day) and sterilized aquadest medium (0,03472 0,00251 /day).

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

Dianthus chinensis L. is an edible, ornamental herb used to prepare the Dianthi Herba, a Chinese traditional rejuvenating medicine. Owing to the rapid proliferation of callus tissues, in vitro production of flavonoids has their own specific importance. Callus cultures raised followed by auxin directed biosynthesis of flavonoid through related transcript profile were carried out. Murashige and Skoog (MS) medium fortified with 2,4- Dichlorophenoxy acetic acid (2,4- D) or picloram induced formation of friable callus from internode derived cultures of D. chinensis. Culture medium containing 2,4- D (10 µM) produced the highest flavonoid content, 4.44 mg quercetin equivalent per gram (QE g− 1) under incubation in continuous dark condition, while maximum dry weight yield (0.38 g/ culture) was obtained from 10 µM 2,4- D under 16 h light / 8 h dark condition (50 µmol m− 2 s− 1 irradiance) at 60 days of incubation. The callus raised in light condition in 10 µM 2,4- D selected to analyze flavonoid related gene expression profile viz., chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3H), and flavonol synthase (FLS) at specific time intervals. The transcript abundance of CHS, F3H, or FLS gene was higher at 60 days old callus cultures and reaching 11.59, 48.31, and 114.63-fold relative expression than that of initial callus tissues respectively. These understandings are critical for the regulation of targeted phytochemicals as well as their wide exploitation in the field of biological research.