OPTIMIZATION OF BACTERIAL CELLULOSE PRODUCTION FROM SUGAR BEET MOLASSES BY GLUCONACETOBACTER XYLINUS NRRL B-759 IN STATIC CULTURE

We investigated the optimization of bacterial cellulose (BC) production from sugar beet molasses by Gluconacetobacter xylinus NRRL B-759 in static culture. The optimization studies were performed using the central composite design (CCD) of response surface methodology (RSM). The independent variables were the molasses concentration, inoculation ratio and culture volume. The dependent variable was BC production yield. From the optimization tests, based on the model developed by RSM-ANOVA, it was found that binary interactions between molasses concentration–culture volume and inoculation ratio–culture volume had the most significant influence on the responses. The optimum conditions were as follows: 78.932 g/L molasses concentration, 12.973% inoculation ratio, and 130.405 mL of culture volume. The obtained BC was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and elemental analysis. The characterization results obtained in the study revealed that the produced BC exhibited typical FTIR spectrum, elemental composition, and nanofiber structure.

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Preparation and characterization of cellulose nanocrystals from bacterial cellulose produced in sugar beet molasses and cheese whey media

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2018.10.136 ◽

2019 ◽

Vol 122 ◽

pp. 280-288 ◽

Cited By ~ 29

Author(s):

Mahdieh Salari ◽

Mahmood Sowti Khiabani ◽

Reza Rezaei Mokarram ◽

Babak Ghanbarzadeh ◽

Hossein Samadi Kafil

Keyword(s):

Sugar Beet ◽

Bacterial Cellulose ◽

Cellulose Nanocrystals ◽

Cheese Whey ◽

Beet Molasses ◽

Sugar Beet Molasses

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Optimization of the osmotic dehydration of carrot cubes in sugar beet molasses

Thermal Science ◽

10.2298/tsci110808129m ◽

2012 ◽

Vol 16 (1) ◽

pp. 43-52 ◽

Cited By ~ 8

Author(s):

Nevena Misljenovic ◽

Gordana Koprivica ◽

Lato Pezo ◽

Ljubinko Levic ◽

Biljana Curcic ◽

...

Keyword(s):

Sugar Beet ◽

Treatment Time ◽

Osmotic Dehydration ◽

Matter Content ◽

Fractional Factorial ◽

Dry Matter Content ◽

Optimum Conditions ◽

Solid Gain ◽

Beet Molasses ◽

Sugar Beet Molasses

A Response Surface Methodology approach (RSM) was used to determine optimum conditions for the osmotic dehydration of carrot cubes in sugar beet molasses. Treatment times were set to 1, 3 and 5 h, at temperatures of 45, 55 and 65?C and molasses concentrations were 40, 60 and 80% (w/w). The used responses variables were: final dry matter content (DM), water loss (WL), solid gain (Sg), and water activity (aw). A Box and Behnken?s fractional factorial design (2 level-3 parameter) with 15 runs (1 block) was used for design of the experiment. DM, WL, Sg were significantly affected by all process variables (at 90-95% confidence level). The optimum conditions were determined by superimposing the contour plots, with the following response limiting values: DM 50-60%, WL 0.7- 0.8, Sg 0.08-0.09, and aw 0.84-0.86. The optimum conditions generated were: treatment time of 4h, temperature of 60?C, sugar concentration of 66% (w/w).

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