The synthesized calcium oxide nanoparticles of 24 nm size from domestic chicken eggshell was used for production of liquid fuel (biodiesel) to test its catalytic behavior, whereas the yield of liquid fuel was also ascertained and it shows varying percentage yields base on different conditions used for the production. The highest yield was found to be 80% at catalyst concentration of 1.75 ww % and temperature of 60°C, followed by 69% yield at catalyst concentration of 0.5 and temperature of 60°C, whereas the lowest yield was found to be 58% which was found to be at the exact catalyst concentration of 0.5 and temperature of 60°C.