Development of EST Markers Specific to Agropyron cristatum Chromosome 6P in Common Wheat Background

2013 ◽  
Vol 38 (10) ◽  
pp. 1791-1801 ◽  
Author(s):  
Cheng DAI ◽  
Jin-Peng ZHANG ◽  
Xiao-Yang WU ◽  
Xin-Ming YANG ◽  
Xiu-Quan LI ◽  
...  
Keyword(s):  
Common Wheat ◽  
Agropyron Cristatum ◽  
Chromosome 6P

Physical mapping of Agropyron cristatum chromosome 6P using deletion lines in common wheat background

2016 ◽  
Vol 129 (5) ◽  
pp. 1023-1034 ◽  
Author(s):  
Liqiang Song ◽  
Yuqing Lu ◽  
Jinpeng Zhang ◽  
Cuili Pan ◽  
Xinming Yang ◽  
...  
Keyword(s):  
Common Wheat ◽  
Physical Mapping ◽  
Agropyron Cristatum ◽  
Deletion Lines ◽  
Chromosome 6P

scholarly journals Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat

2018 ◽  
Vol 17 (8) ◽  
pp. 1697-1705 ◽  
Author(s):  
Hong-xin CHEN ◽  
Hai-ming HAN ◽  
Qing-feng LI ◽  
Jin-peng ZHANG ◽  
Yu-qing LU ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Common Wheat ◽  
Agropyron Cristatum

Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

Euphytica ◽  
2014 ◽  
Vol 201 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Virginia Ochoa ◽  
Eva Madrid ◽  
Mahmoud Said ◽  
Diego Rubiales ◽  
Adoración Cabrera
Keyword(s):  
Leaf Rust ◽  
Common Wheat ◽  
Chromosome Translocation ◽  
Agropyron Cristatum ◽  
Cytogenetic Characterization

Cytological and molecular analysis of wheat – Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat

Genome ◽  
2016 ◽  
Vol 59 (10) ◽  
pp. 840-850 ◽  
Author(s):  
Liqiang Song ◽  
Yuqing Lu ◽  
Jinpeng Zhang ◽  
Cuili Pan ◽  
Xinming Yang ◽  
...  
Keyword(s):  
Common Wheat ◽  
Agronomic Traits ◽  
Physical Map ◽  
Wheat Breeding ◽  
Agropyron Cristatum ◽  
Grain Number ◽  
Specific Sequence ◽  
Sts Markers ◽  
Grain Number Per Spike ◽  
Translocation Lines

Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat – A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat – A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome. FISH results showed that 15, 5, and 6 lines were translocated onto wheat A, B, and D genomes, respectively. Compared with the previous reports, a fine physical map of 6P chromosome was constructed, consisting of 31 chromosomal bins with 255 STS markers. Twelve translocation lines containing 6PS13∼14 chromosomal bins were highly resistant to leaf rust. Two lines showed high grain number per spike, and three lines displayed both enhanced grain number per spike and thousand-grain weight. Development of wheat – A. cristatum 6P translocation lines will not only provide novel wheat germplasm for wheat breeding but also be helpful to broaden the genetic basis of common wheat.

scholarly journals New Insights Into the Introgression Between Agropyron Cristatum P Genome and Wheat Genome

2021 ◽  
Author(s):  
Zhi Zhang ◽  
Shenghui Zhou ◽  
Weihua Liu ◽  
Liqiang Song ◽  
Jinpeng Zhang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
In Situ Hybridization ◽  
Common Wheat ◽  
Agronomic Traits ◽  
Wheat Genome ◽  
Agropyron Cristatum ◽  
Translocation Line ◽  
Gene Markers ◽  
Physiological Races

Abstract Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat and confers desirable agronomic traits to common wheat. A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81–1.00) exhibited high resistance to prevalent physiological races (CYR32 and CYR33). In this study, three disease resistance-related transcriptomes, which were mapped to A. cristatum 6PS (0.81–1.00) through the analysis of specific molecular markers, were searched from among A. cristatum full-length transcriptomes. Then, three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH)/genomic in situ hybridization (GISH) probes made from the DNA of three bacterial artificial chromosome (BAC) clones, three genes, and A. cristatum “Z559” were used to analyze the BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655. The results revealed the introgression can spontaneously occur between A. cristatum P genome and wheat genome, and indicated the three genes could constitute a gene cluster according to the positions of their FISH signals. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 wheat-A. cristatum breeding materials, which showed resistance to physiological races (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, our study not only provides new insights into the introgression between A. cristatum P genome and wheat genome, but also provides the desirable breeding materials for breeding practice.

Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length

2015 ◽  
Vol 128 (9) ◽  
pp. 1827-1837 ◽  
Author(s):  
Jing Zhang ◽  
Jinpeng Zhang ◽  
Weihua Liu ◽  
Haiming Han ◽  
Yuqing Lu ◽  
...  
Keyword(s):  
Common Wheat ◽  
Grain Weight ◽  
Spike Length ◽  
Agropyron Cristatum ◽  
Chromosome Segment ◽  
Thousand Grain Weight

scholarly journals Development of P genome-specific SNPs and their application in tracing Agropyron cristatum introgressions in common wheat

2019 ◽  
Vol 7 (2) ◽  
pp. 151-162 ◽  
Author(s):  
Huihui Ma ◽  
Jinpeng Zhang ◽  
Jing Zhang ◽  
Shenghui Zhou ◽  
Haiming Han ◽  
...  
Keyword(s):  
Common Wheat ◽  
Agropyron Cristatum

scholarly journals Sequence elimination in hybrid offspring of wheat-Agropyron cristatum (L.) Gaertn introgression line Pubing3504 × common wheat cultivar Jing4839

2016 ◽  
Author(s):  
Wu Xiaoyang ◽  
Chen Dan ◽  
Lu Yuqing ◽  
Zhang Jinpeng ◽  
Liu Weihua ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Common Wheat ◽  
Wheat Cultivar ◽  
Introgression Line ◽  
Snp Markers ◽  
Agropyron Cristatum ◽  
Chromosome Segment ◽  
Common Wheat Cultivar ◽  
Chromosome Differentiation ◽  
Specific Sequences

ABSTRACTSequence elimination is one of main reasons for homologous chromosome differentiation in common wheat. Sequence elimination can occur in genome-specific sequences, chromosome-specific sequences, and repeat sequences in the wheat genome. Genetic polymorphism loci in chromosome-specific sequences can be used to develop molecular markers including simple sequence repeats (SSRs), insertions and deletions, and single nucleotide polymorphisms (SNPs). Pubing3504 is a wheat-Agropyron cristatum (L.) Gaertn introgression line, and Jing4839 is a common wheat cultivar. Assessment of their recombinant inbred line (RIL) population using 120 pairs of SSR markers covering all wheat chromosomes indicated that sequence elimination occurred at the short arm of chromosome 1A (1AS). We developed 13 pairs of new co-dominant SSR markers and constructed a genetic linkage map of 1AS; we found that the segment with sequence elimination is from SSR110 to the end of 1AS. We further developed 10 pairs of dominant SNP markers of Pubing3504, 10 pairs of dominant SNP markers of Jing4839, and 10 pairs of primers designed in SNP flanking sequences to assess RILs. We found that all chromosome segments with sequence elimination came from Jing4839. The sequence elimination occurred in SSR loci, SNP loci, and coding sequences. There was no homologous recombination in the chromosome segment with sequence elimination. We suggest that sequence elimination causes the differentiation of chromosomes and the chromosome differentiation affects the homologous pairing at the chromosome segment in meiosis, which further affects the occurrence of homologous recombination at the chromosome segment.

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