Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length

The genetic and phenotypic correlations between yield components (productive tillering, plant height, spike length, number of spikelets per spike, number of grains per spike, grain weight per spike, grain weight per plant, harvest index, thousand grain weight) and quality components (grain protein content and sedimentation value) were investigated. The plant material was comprised of 50 genotypes of winter wheat grown during two years. Path- coefficient analysis of genetic correlation coefficients for grain mass/plant and other traits determined interrelationships among grain mass per plant and other yield and bread making quality components. The strongest positive genetic correlation was found between grain weight per spike and thousand grain weight and between spike length and number of spikelets per spike. Phenotypic correlation analysis indicated that grain weight per spike correlated positively and significantly with harvest index and thousand kernel weight. The strongest direct effect on grain weight per plant had harvest index and number of spikelets per spike. The spike length through number of spikelets per spike had the strongest indirect effect on grain weight per plant.

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Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

BMC Genomics ◽

10.1186/s12864-019-6416-4 ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Shenghui Zhou ◽

Jinpeng Zhang ◽

Haiming Han ◽

Jing Zhang ◽

Huihui Ma ◽

...

Keyword(s):

Single Molecule ◽

Sequence Data ◽

Full Length ◽

Grain Weight ◽

Agropyron Cristatum ◽

Reference Sequence ◽

Translocation Line ◽

Thousand Grain Weight ◽

Sequencing Platform ◽

Transcriptome Sequences

Abstract Background Agropyron cristatum (L.) Gaertn. (2n = 4x = 28; genomes PPPP) is a wild relative of common wheat (Triticum aestivum L.) and provides many desirable genetic resources for wheat improvement. However, there is still a lack of reference genome and transcriptome information for A. cristatum, which severely impedes functional and molecular breeding studies. Results Single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) was used to sequence full-length cDNA from a mixture of leaves, roots, stems and caryopses and constructed the first full-length transcriptome dataset of A. cristatum, which comprised 44,372 transcripts. As expected, the PacBio transcripts were generally longer and more complete than the transcripts assembled via the Illumina sequencing platform in previous studies. By analyzing RNA-Seq data, we identified tissue-enriched transcripts and assessed their GO term enrichment; the results indicated that tissue-enriched transcripts were enriched for particular molecular functions that varied by tissue. We identified 3398 novel and 1352 A. cristatum-specific transcripts compared with the wheat gene model set. To better apply this A. cristatum transcriptome, the A. cristatum transcripts were integrated with the wheat genome as a reference sequence to try to identify candidate A. cristatum transcripts associated with thousand-grain weight in a wheat-A. cristatum translocation line, Pubing 3035. Conclusions Full-length transcriptome sequences were used in our study. The present study not only provides comprehensive transcriptomic insights and information for A. cristatum but also proposes a new method for exploring the functional genes of wheat relatives under a wheat genetic background. The sequence data have been deposited in the NCBI under BioProject accession number PRJNA534411.

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Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

10.21203/rs.2.9773/v1 ◽

2019 ◽

Author(s):

Shenghui Zhou ◽

Jinpeng Zhang ◽

Haiming Han ◽

Jing Zhang ◽

Ma Huihui ◽

...

Keyword(s):

Single Molecule ◽

Sequence Data ◽

Triticum Aestivum L ◽

Full Length ◽

Grain Weight ◽

Agropyron Cristatum ◽

Translocation Line ◽

Thousand Grain Weight ◽

Sequencing Platform ◽

Transcriptome Sequences

Abstract Background Agropyron cristatum (L.) Gaertn. (2n = 4x = 28; genomes PPPP) is a wild relative of common wheat (Triticum aestivum L.) and provides many desirable genetic resources of wheat improvement. However, there is still a lack of reference genome and transcriptome information for A. cristatum, which severely impedes functional and molecular breeding studies. Results Single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) was used to sequence full-length cDNA from a mixture of leaves, roots, stems and caryopses and constructed the first full-length transcriptome dataset of A. cristatum, which comprised 44,372 transcripts. As expected, the PacBio transcripts were generally longer than the transcripts assembled via the Illumina sequencing platform in a previous study. By mapping the full-length transcripts in this study, we identified tissue-enriched expressed transcripts and enriched GO terms, indicating that tissue-enriched transcripts were enriched for particular molecular functions that varies with tissue. We identified 3,398 novel and 1,352 A. cristatum-specific transcripts compared with the wheat gene model set. In order to better apply this transcriptome of A. cristatum, the A. cristatum transcripts was integrated with wheat genome as a reference sequences to try to identify candidate A. cristatum transcripts associated with thousand-grain weight in a wheat-A. cristatum translocation line Pubing 3035. Conclusions Full-length transcriptome sequences were used in our study. The present study not only provides comprehensive transcriptomic insights and information for A. cristatum but also proposes a new method for exploring the functional genes of wheat relatives under a wheat genetic background. The sequence data have been deposited in the NCBI under BioProject accession number PRJNA534411.

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Study on Genotypic Response and Correlation Analysis of the Yield and Yield Attributing Traits of Different Barley (Hordeum Vulgare) Genotypes

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v4i4.16269 ◽

2017 ◽

Vol 4 (4) ◽

pp. 529-536

Author(s):

Deepak Vitrakoti ◽

Sheetal Aryal ◽

Santosh Rasaily ◽

Bishnu Raj Ojha ◽

Raju Kharel ◽

...

Keyword(s):

Grain Yield ◽

Leaf Area ◽

Plant Height ◽

Flag Leaf ◽

Grain Weight ◽

Spike Length ◽

Thousand Grain Weight ◽

Days To Flowering ◽

Days To Heading ◽

Peduncle Length

Barley, being a tremendous opportunities crop, we are far back regarding study, research and utilization. An experiment was conducted 2014-2015 to evaluate the barley genotypes for their yield attributing traits and correlation and causation. Eleven yield contributing traits viz., days to booting, heading and flowering; peduncle length, spike length, plant height, flag leaf area, flag leaf-1 area, thousand grain weight, biological weight and yield per hectare were recorded. High significant variation among genotypes was found for traits under study. Genotypes SBYT3-13#1115 (1960 kg), 14-SB-NAK-MR#17 (1760 kg) and AM POP#26 (1660 kg) were found to be superior for their per se performance based on grain yield per hectare, yield attributing and other quantitative traits. Thousand grains weight (0.333) had positively highest significant correlation with grain yield per hectare followed by spike length (0.310). Grain yield per hectare showed negative highly significant correlation with days to flowering (-0.796) followed by days to heading (-0.761) and days to booting (-0.663). Peduncle length (0.229), plant height (0.226), biological weight (0.181) and flag leaf area (0.032) were positively correlated with grain yield per hectare while flag leaf-1 area(-0.029) was negatively correlated. Thus, selection for genotypes with higher thousand grain weight and spike length accommodating earlier days to flowering, heading and booting is a prerequisite for attaining improvement in grain yield per hectare.Int J Appl Sci Biotechnol, Vol 4(4): 529-536

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Pleiotropic function of SQUAMOSA PROMOTER-BINDING PROTEIN-BOX gene TaSPL14 on plant architecture of wheat

10.21203/rs.3.rs-25293/v1 ◽

2020 ◽

Author(s):

Jie Cao ◽

Kaiye Liu ◽

Wanjun Song ◽

Jianing Zhang ◽

Yingyin Yao ◽

...

Keyword(s):

Transcription Factor ◽

Plant Height ◽

Plant Architecture ◽

Grain Weight ◽

Spike Length ◽

Thousand Grain Weight ◽

Spikelet Number ◽

Ethylene Response ◽

Spike Development ◽

Squamosa Promoter Binding Protein

Abstract Background SQUAMOSA PROMOTER-BINDING PROTEIN-BOX gene OsSPL14 from rice is evaluated as the major gene for ideal plant architecture consisting of few unproductive tillers, more grains per spike and high lodging resistance stems. However, the function of its orthologous gene TaSPL14 in wheat is still unknown. Results Here, we reported the similarity and variation between TaSPL14 and OsSPL14. Similar to OsSPL14, TaSPL14 knock-out mutants exhibited decreased plant height, spike length, spikelet number, thousand-grain weight. Different from OsSPL14, TaSPL14 had no effect on tiller number. Transcriptome analysis genes related to ethylene response were significantly decreased in young spikes of TaSPL14 knockout mutants, compared with wild type. TaSPL14 directly binds to the promoters of the ethylene response gene TaEIL1 (EIN3-LIKE 1), TaRAP2.11 (ETHYLENE-RESPNSIVE TRANSCRIPTION FACTOR 2.11) and TaERF1 (ETHYLENE-RESPNSIVE TRANSCRIPTION FACTOR 1) and activities their expression, suggesting that TaSPL14 might regulate wheat spike development through ethylene response pathway. Conclusions TaSPL14 had similar function with OsSPL14 in regulating plant height, spike length, spikelet number and thousand-grain weight of wheat, and had different function in tiller development. TaSPL14 might regulate spike development through TaEIL1, TaRAP2.11 and TaERF1, not TaDEP1.The elucidation of TaSPL14 will contribute to exploring the molecular mechanisms underlying plant architecture of wheat.

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Sequence elimination in hybrid offspring of wheat-Agropyron cristatum (L.) Gaertn introgression line Pubing3504 × common wheat cultivar Jing4839

10.1101/067504 ◽

2016 ◽

Author(s):

Wu Xiaoyang ◽

Chen Dan ◽

Lu Yuqing ◽

Zhang Jinpeng ◽

Liu Weihua ◽

...

Keyword(s):

Homologous Recombination ◽

Common Wheat ◽

Wheat Cultivar ◽

Introgression Line ◽

Snp Markers ◽

Agropyron Cristatum ◽

Chromosome Segment ◽

Common Wheat Cultivar ◽

Chromosome Differentiation ◽

Specific Sequences

ABSTRACTSequence elimination is one of main reasons for homologous chromosome differentiation in common wheat. Sequence elimination can occur in genome-specific sequences, chromosome-specific sequences, and repeat sequences in the wheat genome. Genetic polymorphism loci in chromosome-specific sequences can be used to develop molecular markers including simple sequence repeats (SSRs), insertions and deletions, and single nucleotide polymorphisms (SNPs). Pubing3504 is a wheat-Agropyron cristatum (L.) Gaertn introgression line, and Jing4839 is a common wheat cultivar. Assessment of their recombinant inbred line (RIL) population using 120 pairs of SSR markers covering all wheat chromosomes indicated that sequence elimination occurred at the short arm of chromosome 1A (1AS). We developed 13 pairs of new co-dominant SSR markers and constructed a genetic linkage map of 1AS; we found that the segment with sequence elimination is from SSR110 to the end of 1AS. We further developed 10 pairs of dominant SNP markers of Pubing3504, 10 pairs of dominant SNP markers of Jing4839, and 10 pairs of primers designed in SNP flanking sequences to assess RILs. We found that all chromosome segments with sequence elimination came from Jing4839. The sequence elimination occurred in SSR loci, SNP loci, and coding sequences. There was no homologous recombination in the chromosome segment with sequence elimination. We suggest that sequence elimination causes the differentiation of chromosomes and the chromosome differentiation affects the homologous pairing at the chromosome segment in meiosis, which further affects the occurrence of homologous recombination at the chromosome segment.

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Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

10.21203/rs.2.9773/v2 ◽

2019 ◽

Author(s):

Shenghui Zhou(Former Corresponding Author) ◽

Jinpeng Zhang ◽

Haiming Han ◽

Jing Zhang ◽

Ma Huihui ◽

...

Keyword(s):

Single Molecule ◽

Sequence Data ◽

Full Length ◽

Grain Weight ◽

Agropyron Cristatum ◽

Reference Sequence ◽

Translocation Line ◽

Thousand Grain Weight ◽

Sequencing Platform ◽

Transcriptome Sequences

Abstract Agropyron cristatum (L.) Gaertn. (2n = 4x = 28; genomes PPPP) is a wild relative of common wheat (Triticum aestivum L.) and provides many desirable genetic resources for wheat improvement. However, there is still a lack of reference genome and transcriptome information for A. cristatum, which severely impedes functional and molecular breeding studies.Results Single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) was used to sequence full-length cDNA from a mixture of leaves, roots, stems and caryopses and constructed the first full-length transcriptome dataset of A. cristatum, which comprised 44,372 transcripts. As expected, the PacBio transcripts were generally longer and more complete than the transcripts assembled via the Illumina sequencing platform in previous studies. By analyzing RNA-Seq data, we identified tissue-enriched transcripts and assessed their GO term enrichment; the results indicated that tissue-enriched transcripts were enriched for particular molecular functions that varied by tissue. We identified 3,398 novel and 1,352 A. cristatum-specific transcripts compared with the wheat gene model set. To better apply this A. cristatum transcriptome, the A. cristatum transcripts were integrated with the wheat genome as a reference sequence to try to identify candidate A. cristatum transcripts associated with thousand-grain weight in a wheat-A. cristatum translocation line, Pubing 3035.Conclusions Full-length transcriptome sequences were used in our study. The present study not only provides comprehensive transcriptomic insights and information for A. cristatum but also proposes a new method for exploring the functional genes of wheat relatives under a wheat genetic background. The sequence data have been deposited in the NCBI under BioProject accession number PRJNA534411.

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Influence des rayons gamma sur les parametres morphologiques et agronomiques de semences d’une variete de ble tendre cultivee en Algerie

African Journal of Food, Agriculture, Nutrition and Development ◽

10.18697/ajfand.104.20195 ◽

2021 ◽

Vol 21 (9) ◽

pp. 18767-18783

Author(s):

Samia Fernane-Meliani ◽

◽

Z Abdellaoui ◽

S Akretche-Kelfat ◽

B Ancer ◽

...

Keyword(s):

Grain Yield ◽

Common Wheat ◽

Gamma Rays ◽

Yield Components ◽

Grain Weight ◽

Spike Length ◽

Growth And Yield ◽

Wheat Crop ◽

Grand Nombre ◽

1000 Grain Weight

RESUME: L'objectif de cette étude était de déterminer les effets de diverses doses de rayons gamma sur les paramètres morphologiques et agronomiques de semences de blé (Anza). L’expérimentation a été menée à la station expérimentale de l’Ecole Nationale Supérieure Agronomique, El Harrach (Alger, Algérie), sur une parcelle en bloc aléatoire complet, en évaluant la croissance et les composantes de rendement d’une culture, sur deux années consécutives, de cette variété locale de blé tendre (Anza) cultivée en Algérie. Les graines ont été irradiées avec des rayons gamma de 0,10, 0,15 et 0,20 KGy (KiIoGry), tandis que la dose de 0KGy a été retenue comme témoin. Les résultats ont montré que la germination n'a pas été affectée par l'irradiation. La hauteur de la tige la plus marquée a été donnée par le témoin pendant les deux années de culture. A la première année, la dose de 0,20KGy et le témoin ont manifesté la meilleure longueur de l’épi (8,97cm) et le plus grand nombre de pieds levés/m2 a été noté à la deuxième année avec le blé traité à 0,20KGy (149). Le nombre le plus élevé d’épis /m2 est obtenu par les échantillons irradiés à 0,20 KGy et le témoin a donné le plus grand nombre de grains par épi, alors qu’une amélioration de la masse de 1000 grains a été constatée à 0,20KGy. La dose de 0,20KGy a également produit le meilleur rendement en grains à la récolte des deux année (56 et 55q/ha). Il y a eu une interaction entre l'année de culture et la dose d’irradiation pour le nombre de pieds levés et le nombre d’épis/m2. Les faibles doses de rayons gamma, notamment la dose de 0,20 KGy ont contribué à l’amélioration des caractéristiques morphologiques et agronomiques de la variété locale de blé tendre (Anza) cultivée en Algérie, à savoir : la longueur de l’épi, le nombre de pieds levés et d’épis/m2, la masse de 1000 grains et le rendement en grains. ABSTRACT: The aim of this study was to determine the effect of different doses of gamma rays on morphological and agronomical parameters of wheat seeds (Anza). The experiment was conducted in the experimental station of the National School of Agricultural Sciences, El Harrach, Algiers (Algeria). The wheat crop was carried out on a plot in complete random block, by evaluating the growth and yield components of a crop, over two consecutive years, of this common wheat variety (Anza) grown in Algeria. Seeds samples were irradiated with low doses of gamma rays at 0.10, 0.15 and 0.20 KGy (KiIoGry), while 0KGy dose was used as a control. The results showed that the number of germinated seeds was not affected by the irradiation treatment. The highest stem height was given by the control in both years, whereas in the first crop year, the 0.20KGy dose with the control showed the best spike length (8.97cm). In the second year, the highest number of raised feet/m2 was recorded with wheat at 0.20KGy (149). The evolution of yield components according to irradiation doses revealed that the highest number of spikes/m2 was also obtained from samples irradiated at 0.20KGy, the control gave the highest number of seeds by spike, while an improvement in 1000 grain weight was seen at 0.20KGy in both crops. The 0.20KGy dose also produced the highest grain yield at harvest of the two years (56 and 55q/ha). There was an interaction between the crop year and the radiation dose for the number of raised feet and the number of spikes/m2. The low gamma ray doses, particularly 0.20 KGy dose, contributed to the improvement of the morphological and agronomical properties of the local common wheat (Anza) grown in Algeria, including spike length, number of raised feet and spikes/m2, 1000 grain weight and grain yield.

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