Phase Transitions in Phospholipid Monolayers Studied by Atomic Force Microscopy and Langmuir-Blodgett Technique

2008 ◽  
Vol 59 (11) ◽  
Author(s):  
Maria Tomoaia-Cotisel ◽  
Aurora Mocanu

The phase behaviour and surface structure of dipalmitoyl phosphatidyl choline (DPPC) monolayers at the air/water interface, in the absence and the presence of procaine, have been investigated by Langmuir-Blodgett (LB) technique and atomic force microscopy. The LB films were transferred on mica, at a controlled surface pressure, characteristic for the expanded liquid to condensed liquid phase transition of pure DPPC monolayers. The results indicate that procaine penetrates into and specifically interacts with phospholipid monolayers stabilizing the lipid membrane interface.

1996 ◽  
Vol 440 ◽  
Author(s):  
H. C. Wang ◽  
D. W. Cheong ◽  
J. Kumar ◽  
C. Sung ◽  
S. K. Tripathy

AbstractA soluble, asymmetrically substituted polydiacetylene, poly(BPOD), has been reported to form stable monolayers at the air-water interface by the Langmuir-Blodgett (LB) technique [2]. Preformed polydiacetylene has been deposited onto hydrophobic substrates as multilayers to form second order nonlinear optical thin films. Second harmonic generation was found to increase with the number of layers. From previous atomic force microscopy (AFM) studies backbone orientation along the dipping direction with an interchain spacing of about 5 A° was indicated [2].The film morphology and preferential molecular orientation of these LB films are further investigated by transmission electron microscopy (TEM). A specifically tailored sample preparation method for the ultrathin LB films was used. Multilayer films were deposited on hydrophobic collodion covered glass substrates for this purpose. Electron diffraction was employed to study the crystalline organization of mono and multilayers of LB films as well as cast films.


2010 ◽  
Vol 132 (16) ◽  
pp. 5604-5606 ◽  
Author(s):  
Jiro Kumaki ◽  
Takashi Kajitani ◽  
Kanji Nagai ◽  
Kento Okoshi ◽  
Eiji Yashima

1993 ◽  
Vol 57 (1) ◽  
pp. 3795-3800 ◽  
Author(s):  
J. Garnaes ◽  
D.K. Schwartz ◽  
R. Viswanathan ◽  
J.A.N. Zasadzinski

Nature ◽  
1991 ◽  
Vol 349 (6308) ◽  
pp. 398-400 ◽  
Author(s):  
E. Meyer ◽  
L. Howald ◽  
R. M. Overney ◽  
H. Heinzelmann ◽  
J. Frommer ◽  
...  

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