scholarly journals Metal Cations Analysis Contained in Sambucus ebulus L., by Comparative Methods of Atomic Absorption Spectrometry

2019 ◽  
Vol 70 (9) ◽  
pp. 3225-3227
Author(s):  
Adina Magdalena Bunget ◽  
Michael Schenker ◽  
Alin Dragos Demetrian ◽  
Aurel Gherghina ◽  
Lucretiu Radu ◽  
...  
Keyword(s):  
Nitric Acid ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Metal Cations ◽  
Absorption Spectrometry ◽  
Microwave Oven ◽  
The Other ◽  
Vegetative Organs ◽  
Sample Digestion ◽  
Sambucus Ebulus

The purpose of this study was to perform a comparative study of cations in the vegetative organs of Sambucus ebulus by applying three different methods of digestion before atomic absorption spectrometry. Of the tested methods, the most effective was method 3 (mineralisation with nitric acid in a microwave oven), the other methods of sample digestion leading to incomplete mineralization of the samples and implicitly to the incomplete transfer of the analytes into solution, reflecting the slightly different contents in metal cations we obtained.

Zinc determined in 10-microL serum or urine samples by flameless atomic absorption spectrometry.

1981 ◽  
Vol 27 (1) ◽  
pp. 73-77 ◽  
Author(s):  
N E Vieira ◽  
J W Hansen
Keyword(s):  
Nitric Acid ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Absorption Spectrometry ◽  
Repeated Measurements ◽  
Spectrometric Method ◽  
Flameless Atomic Absorption ◽  
Standard Curve ◽  
Flame Atomic Absorption ◽  
Calibration Algorithm

Abstract We describe a precise flameless atomic absorption spectrometric method requiring only 10 microL of sample, which thus permits repeated measurements in the neonate. Standard curves covering the range of 0 to 30 mg of zinc per liter, with standards in various matrixes (albumin, glycerol, serum, or nitric acid) had slopes ranging from 0.49 to 1.17, relative to that for aqueous standards. We prepared low-zinc matrixes, which had slopes similar to that of serum, by dialyzing serum or a 50 g/L solution of albumin vs a buffered zinc-free dialysis fluid containing appropriate inorganic constituents. Use of thoroughly de-ionized water, reagent-grade chemicals, "ultrapure" nitric acid, specified disposable plastic ware, and appropriate pipet tip-rinsing techniques minimized extraneous contamination with zinc. Concentrations of zinc in serum calculated from a "rational-method" calibration algorithm fit to the standard curve agreed well with independent determinations by flame atomic absorption spectrometry.

scholarly journals Chemical Composition of Seeds of Two Endemic Lathyrus L. Species from Turkey

2018 ◽  
Vol 9 (2) ◽  
pp. 66
Author(s):  
Yasin ARSLAN ◽  
Diğdem TRAK ◽  
Bekir YILDIRIM ◽  
Hasan GENÇ ◽  
Erdal KENDÜZLER
Keyword(s):  
Chemical Composition ◽  
Reference Material ◽  
Atomic Absorption Spectrometry ◽  
Endemic Species ◽  
Contaminated Soil ◽  
Confidence Level ◽  
Absorption Spectrometry ◽  
Microwave Oven ◽  
Plant Taxonomy ◽  
Sample Digestion

Genus Lathyrus L. belonging to Fabaceae (Leguminosae) family consisted of 77 taxa at the species, subspecies, and variety level at Turkey and 25 of that were known as endemic. Lathyrus egirdiricus H.Genc & A.Sahin and Lathyrus tefennicus H. Genç & A. Şahin were endemic species and described at the date of 2008 and 2011, respectively. The seeds of Lathyrus tefennicus and Lathyrus egirdiricus were collected in Tefenni of Burdur province and Eğirdir of Isparta province in May of 2016 at Turkey. The concentration of Cd, Cr, Ni, Cu, Mn, Zn, Fe, Ca, Mg, Na and K in the Lathyrus tefennicus and Lathyrus egirdiricus seeds were determined by Atomic Absorption Spectrometry. A microwave oven was used for the sample digestion. The accuracy of proposed method was confirmed by the analysis of the certified reference material (EnviroMAT Contaminated Soil). All results were given at 95% of confidence level. The presence of different concentrations of elements in the seeds of both species strengthens the belief that this data can be used in plant taxonomy.

scholarly journals Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS)

2021 ◽  
Vol 46 (1) ◽  
pp. 12-20
Author(s):  
Carla Maíra Bossu ◽  
Rodrigo Giglioti ◽  
Marcia Cristina de Sena Oliveira ◽  
Pedro Vitoriano De Oliveira ◽  
Ana Rita de Araujo Nogueira
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Gel Electrophoresis ◽  
Screening Method ◽  
Electrothermal Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Microwave Oven ◽  
Soybean Milk ◽  
Milk Samples ◽  
Casein Protein

A screening method for zinc levels in different milk samples (raw cow, raw sheep, UHT cow, UHT goat and soybean milk base) was performed to establish the Zn levels’ differences in protein samples. The samples were digested in a cavity microwave oven and the total Zn levels in the extracts were determined by flame absorption atomic spectrometry (FAAS). The protein separation was performed by urea polyacrylamide gel electrophoresis (UREA-PAGE). Protein bands were digested in the cavity microwave oven and Zn-protein analysis was further conducted by electrothermal atomic absorption spectrometry (ETAAS). The results showed that Zn is mainly bound to 32 kDa (β-casein) protein (17.0 ± 2.0%) in UHT cow and 24 kDa (α-casein) protein (9.0 ± 0.6%) in raw sheep milk. This method provided quantitative information regarding Zn species present in the protein fractions of the milk samples. The accuracy was evaluated using certified reference material (whole milk powder, NIST 8435) with statistically equivalent concentrations (Student’s t-test) for total Zn and by addition and recovery experiments applied to measure Zn-protein. The recovered values were in the 92-110% range.

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