Changes in Pectic Substances of Mature-Green Mume (Prunus mume Sieb. et Zucc) Fruit as Influenced by the Thickness of Packaging Film during Storage

2002 ◽  
Vol 31 (4) ◽  
pp. 621-628 ◽  
1993 ◽  
Vol 40 (3) ◽  
pp. 163-169 ◽  
Author(s):  
Shiming ZHANG ◽  
Kazuo CHACHIN ◽  
Yoshinori UEDA ◽  
Takashi IWATA

Author(s):  
J. H. Luft

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JT Hwang ◽  
HJ Yang ◽  
HJ Hur ◽  
JH Park

1995 ◽  
Vol 64 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Takeshi Ozaki ◽  
Takehiko Shimada ◽  
Tetsu Nakanishi ◽  
Jin Yamamoto ◽  
Masao Yoshida
Keyword(s):  

2005 ◽  
Vol 10 (4) ◽  
pp. 311-315 ◽  
Author(s):  
Seong-Chun Jo ◽  
Ki-Chang Nam ◽  
Byoung-Rok Min ◽  
Dong-Uk Ahn ◽  
Sung-Hwan Cho ◽  
...  

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