Study on Antibacterial Activity of Propolis on Propionibacterium acnes as an Acne-Induced Bacteria

2020 ◽  
Vol 49 (6) ◽  
pp. 586-591
Author(s):  
Na-Rae Kim ◽  
Seung Wan Lee ◽  
Mee Ree Kim
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals The potential of bacteriocin AS-48 in the control of Propionibacterium acnes

2016 ◽  
Author(s):  
Rubén Cebrián ◽  
Sergio Arévalo ◽  
Samir Ananou ◽  
Salvador Arias-Santiago ◽  
Cristina Riazzo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Chemical Compounds ◽  
Clinical Isolates ◽  
Large Decrease ◽  
Large Problem ◽  
Specific Pcr ◽  
Therapeutic Drugs ◽  
Dermatological Disease

Background Global reports show that the antimicrobial-resistance of Propionibacterium acnes isolated from patients with acne vulgaris is becoming a large problem, making it necessary to find new therapeutic drugs. Methods In this study, 23 clinical isolates of P. acnes have been identified by MaldiToff and specific PCR. The susceptibility of theses strains to antibiotics (clindamicin, erytromycin and tetracicline) and to bacteriocin (AS-48) has been established, using the CECT 5684 strain as reference. Moreover, we have investigated the potential of several chemical compounds to bolster the activity of AS-48. Finally, the effectivity of four different formulations containing AS-48 and lysozyme have been evaluated on the surface of swine-ear skin previously inoculated with P. acnes CECT5684 strain. Results. The results presented in this work probe that AS-48 has a significant bactericidal activity against the 23 clinical isolates of P. acnes, including isolates resistant to one or more common antibiotics used in the treatment of acne. Antibacterial synergy of AS-48 with other chemical compounds has been demonstrated, as was the effect of lysozyme and to a lesser extent with palmitic acid. Likewise, the use of a combination therapy into a cream formulation, resulted in large decrease in the number of viable P. acnes counts in an experiemental model. Conclusion. Once more these studios support that compositions comprising bacteriocins displaying antibacterial activity, must be considered an approach for medical and pharmaceutical purposes. These applications are particularly promising in light of emerging antibiotic resistance across bacteria involved in treatment of dermatological disease as acne vulgaris.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE SP.) AND HATI TANAH (ANGIOPTERIS SP.) AND THEIR COMBINATION AGAINST PROPIONIBACTERIUM ACNES

2019 ◽  
pp. 11-13
Author(s):  
SUSI NOVARYATIIN
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
The Other ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Other Hand ◽  
Diffusion Technique ◽  
Inhibition Zones

Objective: The objective of this research was to investigate the preliminary phytochemical screening and antibacterial activity of Bawang Dayak(Eleutherine sp.) and Hati Tanah (Angiopteris sp.) and their combination against Propionibacterium acnes.Methods: The extracts were used for phytochemical screening. Antibacterial activity was performed using disc diffusion technique, with twovariations of the concentration of 5% and 10% for each extracts, and combination of both extracts with three combinations: (1) 5%: 5%, (2) 5%: 10%, and (3) 10%: 5%.Results: Both extracts contained tannins, saponins, and steroids. The antibacterial activity against P. acnes showed that the inhibition zones ofBawang Dayak ethanol extract were 6.1±1.5 mm (5%) and 8.7±1.3 mm (10%). On the other hand, the inhibition zones of Hati Tanah ethanol extract were 4.0±1.6 mm (5%) and 9.2±2.5 mm (10%). The inhibition zones produced in combinations 1, 2, and 3 were 5.8±0.3 mm, 10.8±2.0 mm, and 15.5±2.8 mm, respectively.Conclusion: In this study showed the presence of tannins, saponins, and steroids that might be responsible for antibacterial activity in both extract. The best antibacterial activity was produced by combining the two extracts (combination 3).

scholarly journals THE ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE BULBOSA (MILL.) URB.) FROM CENTRAL KALIMANTAN AGAINST ACNE-CAUSING BACTERIA

2019 ◽  
pp. 22-25
Author(s):  
SUSI NOVARYATIIN ◽  
SYAHRIDA DIAN ARDHANY
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Disc Diffusion ◽  
Central Kalimantan ◽  
Diffusion Technique ◽  
Inhibition Zones ◽  
Phytochemical Constituents

Objective: The objective of this research was to investigate the antibacterial activity of bawang dayak from Central Kalimantan against acne-causingbacteria namely Propionibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus.Methods: The preliminary phytochemical constituents were qualitatively analyzed. Antibacterial activity of bawang dayak ethanol extract wasperformed using disc-diffusion technique, with five variations of concentration of 1.25%, 2.5%, 5%, 10%, and 20%.Results: Bawang dayak extract contained flavonoids, alkaloids, saponins, and tannins. The inhibition zones of bawang dayak ethanol extract in fivevarious concentrations (1.25%, 2.5%, 5%, 10%, and 20%) were 7.0±1.3 mm, 8.5±0.6 mm, 9.9±0.3 mm, 11.2±0.1 mm, and 11.9±0.3 mm against P. acnes;18.8±3.3 mm, 21.9±3.3 mm, 20.8±0.6 mm, 22.0±0.2 mm, and 23.1±0.6 mm against S. epidermidis; and 14.3±3.1 mm, 13.5±0.9 mm, 14.7±1.5 mm,16.1±1.0 mm, and 20.1±0.6 mm against S. aureus, respectively.Conclusion: This present study showed that bawang dayak ethanol extract was active against all the tested acne-causing bacteria. The highestantibacterial activity was produced by 20% of bawang dayak ethanol extract against S. epidermidis.

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