What grafting materials produce greater new bone formation in maxillary sinus floor elevation surgery? A systematic review and network meta-analysis protocol

Abstract BackgroundThe existence of mesenchymal stem cells (MSCs) in Schneiderian membrane has not been determined. The aim of this study is to investigate whether there are MSCs in Schneiderian membrane, and the effect of platelet-rich fibrin (PRF) on osteogenic differentiation of these cells and on new bone formation in maxillary sinus after maxillary sinus floor elevation.MethodsSchneiderian membrane derived mesenchymal stem cells (SM-MSCs) were isolate from rabbit maxillary sinus. Cells were identified by flow cytometry and multipotential differentiation. Real-time cell analysis assay, fluorescence staining, transwell assay, and wound healing assay were used to determine the effects of PRF stimulation on cell proliferation and migration. The osteogenic differentiation ability of cells stimulated by PRF or osteoinductive medium (OM) was evaluated by alkaline phosphatase (ALP) staining, alizarin red staining, PCR and Western blot. Equivalent volume Bio-oss and the mixture of Bio-oss and PRF were used as bone graft materials for maxillary sinus floor elevation. Micro-CT, bone double-staining, HE staining, Masson staining, and toluidine blue staining were used to evaluate the osteogenic effect in 8 and 12 weeks after surgery.ResultsThe cell surface markers were positive for expression of CD90, CD105, and negative for expression of CD34, CD45. SM-MSCs had the ability of osteogenic, adipogenic and chondrogenic differentiation. PRF can stimulate proliferation, migration and osteogenic differentiation of SM-MSCs, which is achieved by up-regulating ERK 1/2 signaling pathway. PRF can accelerate the formation of new bone in maxillary sinus and increase the amount of new bone formation. ConclusionsMSCs exist in Schneiderian membrane, and PRF stimulation can promote cell proliferation, migration and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation can accelerate bone healing and increase the quantity and quality of new bone.

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Maxillary Sinus Floor Augmentation with Autogenous Bone Graft Alone Compared with Alternate Grafting Materials: a Systematic Review and Meta-Analysis Focusing on Histomorphometric Outcome

Journal of Oral and Maxillofacial Research ◽

10.5037/jomr.2020.11302 ◽

2020 ◽

Vol 11 (3) ◽

Author(s):

Thomas Starch-Jensen ◽

Daniel Deluiz ◽

Niels Henrik Bruun ◽

Eduardo Muniz Barretto Tinoco

Keyword(s):

Systematic Review ◽

Bone Graft ◽

Maxillary Sinus ◽

Meta Analysis ◽

Autogenous Bone ◽

Sinus Floor Augmentation ◽

Maxillary Sinus Floor ◽

Sinus Floor ◽

Grafting Materials ◽

Maxillary Sinus Floor Augmentation

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Comparison of Bovine Bone-Autogenic Bone Mixture Versus Platelet-Rich Fibrin for Maxillary Sinus Grafting: Histologic and Histomorphologic Study

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-16-00104 ◽

2017 ◽

Vol 43 (3) ◽

pp. 194-201 ◽

Cited By ~ 11

Author(s):

Hakan Ocak ◽

Nukhet Kutuk ◽

Umut Demetoglu ◽

Esra Balcıoglu ◽

Saim Ozdamar ◽

...

Keyword(s):

Bone Formation ◽

Maxillary Sinus ◽

Autogenous Bone ◽

Bovine Bone ◽

Sinus Floor Elevation ◽

Maxillary Sinus Floor ◽

Sinus Floor ◽

Group 2 ◽

Maxillary Sinus Floor Elevation ◽

Group 1

Numerous grafting materials have been used to augment the maxillary sinus floor for long-term stability and success for implant-supported prosthesis. To enhance bone formation, adjunctive blood-born growth factor sources have gained popularity during the recent years. The present study compared the use of platelet-rich fibrin (PRF) and bovine-autogenous bone mixture for maxillary sinus floor elevation. A split-face model was used to apply 2 different filling materials for maxillary sinus floor elevation in 22 healthy adult sheep. In group 1, bovine and autogenous bone mixture; and in group 2, PRF was used. The animals were killed at 3, 6, and 9 months. Histologic and histomorphologic examinations revealed new bone formation in group 1 at the third and sixth months. In group 2, new bone formation was observed only at the sixth month, and residual PRF remnants were identified. At the ninth month, host bone and new bone could not be distinguished from each other in group 1, and bone formation was found to be proceeding in group 2. PRF remnants still existed at the ninth month. In conclusion, bovine bone and autogenous bone mixture is superior to PRF as a grafting material in sinus-lifting procedures.

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