scholarly journals Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

10.3791/1447 ◽  
2009 ◽  
Author(s):  
Brad Hamilton ◽  
Qiang Feng ◽  
Mike Ye ◽  
G Grant Welstead
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Lentiviral Transduction ◽  
Induced Pluripotent

scholarly journals Differences between Cellular and Molecular Profiles of Induced Pluripotent Stem Cells Generated from Mouse Embryonic Fibroblasts

2010 ◽  
Vol 12 (6) ◽  
pp. 627-639 ◽  
Author(s):  
Eun Young Kim ◽  
Kilsoo Jeon ◽  
Hyo Young Park ◽  
Young Joon Han ◽  
Byoung Chul Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Molecular Profiles ◽  
Induced Pluripotent

scholarly journals Sirtuin 1 Facilitates Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts through the miR-34a and p53 Pathways

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e45633 ◽  
Author(s):  
Yin Lau Lee ◽  
Qian Peng ◽  
Sze Wan Fong ◽  
Andy C. H. Chen ◽  
Kai Fai Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Sirtuin 1 ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Induced Pluripotent

Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction

2019 ◽  
pp. 63-76
Author(s):  
Xiaodong Liu ◽  
Joseph Chen ◽  
Jaber Firas ◽  
Jacob M. Paynter ◽  
Christian M. Nefzger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Lentiviral Transduction ◽  
Induced Pluripotent

scholarly journals Single Transcription Factor Reprogramming of Hair Follicle Dermal Papilla Cells to Induced Pluripotent Stem Cells

Stem Cells ◽  
2011 ◽  
Vol 29 (6) ◽  
pp. 964-971 ◽  
Author(s):  
Su-Yi Tsai ◽  
Britta Am Bouwman ◽  
Yen-Sin Ang ◽  
Soo Jeong Kim ◽  
Dung-Fang Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Induced Pluripotent Stem Cells ◽  
Hair Follicle ◽  
Pluripotent Stem Cells ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Induced Pluripotent

Optimization of Lentiviral Transduction Procedure for the Generation of Human Induced Pluripotent Stem Cells

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Fatemeh Movahedi Motlagh ◽  
Bahareh Rajaei ◽  
Mohammad Massumi ◽  
Maryam Kabir Salmani ◽  
Hamid Reza Soleimanpour-lichaei
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Lentiviral Transduction ◽  
Induced Pluripotent

scholarly journals Glycolysis Combined with Core Pluripotency Factors to Promote the Formation of Chicken Induced Pluripotent Stem Cells

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 425
Author(s):  
Xia Yuan ◽  
Chen Zhang ◽  
Ruifeng Zhao ◽  
Jingyi Jiang ◽  
Xiang Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Embryonic Fibroblasts ◽  
Pluripotency Factors ◽  
Induction Strategy ◽  
Induction Process ◽  
Regulation Mechanisms ◽  
Induced Pluripotent

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) in vitro. Previously, a lentivirus induction strategy of introducing Oct4, Sox2, Nanog and Lin28 (OSNL) into the iPSC process has been shown as a possible way to produce chicken iPSCs from chicken embryonic fibroblasts, but the induction efficiency of this method was found to be significantly limiting. In order to help resolve this efficiency obstacle, this study seeks to clarify the associated regulation mechanisms and optimizes the reprogramming strategy of chicken iPSCs. This study showed that glycolysis and the expression of glycolysis-related genes correlate with a more efficient reprogramming process. At the same time, the transcription factors Oct4, Sox2 and Nanog were found to activate the expression of glycolysis-related genes. In addition, we introduced two small-molecule inhibitors (2i-SP) as a “glycolysis activator” together with the OSNL cocktail, and found that this significantly improved the induction efficiency of the iPSC process. As such, the study identifies direct molecular connections between core pluripotency factors and glycolysis during the chicken iPSC induction process and, with its results, provides a theoretical basis and technical support for chicken somatic reprogramming.

scholarly journals ID: 1021 Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector

2017 ◽  
Vol 4 (S) ◽  
pp. 96
Author(s):  
Oanh Thuy Huynh ◽  
Mai Thi-Hoang Truong ◽  
Phuc Van Pham
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Viral Vector ◽  
Embryonic Stem ◽  
Embryonic Fibroblasts ◽  
Induced Pluripotent ◽  
Teratoma Formation

Background: Embryonic stem cells are pluripotent, thus capable of differentiating into all types of cells derived from the three germ layers. However, the application of embryonic stem cells (ESCs) for preclinical and clinical studies is difficult due to ethical concerns. Induced pluripotent stem cells (iPSCs) are derived from differentiation and have many ESC characteristics. The study herein examines the production of iPSCs from reprogramming of mouse embryonic fibroblasts (MEFs) via transduction with defined factors.  Methods: MEFs were collected from mouse embryos via a previously published protocol. The cells were transduced with a single polycistronic viral vector encoding mouse cDNAs of Oct3/4, Sox2, Klf4 and c-Myc. Transduced cells were treated and sub- cultured with ESC medium. The cells were evaluated as iPSCs with specific morphology, and expression SSEA-1, Oct3/4, Sox2 and Nanog. In addition, they were also evaluated for pluripotency by assessing alkaline phosphatase (AP) activity and in vivo teratoma formation.  Results: Under the reprogrammed conditions, the transduced cells displayed a change in morphology, forming ESC-like clusters. These cell clusters strongly expressed pluripotent markers as well as ESC-specific genes. Furthermore, the colonies exhibited higher AP activity and formed teratomas when injected into the murine testis.  Conclusion: The study herein suggests that MEFs can be reprogrammed into iPSCs using a polycistronic viral vector encoding mouse cDNAs for Oct3/4, Sox2, Klf4 and c- Myc

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