scholarly journals Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion

10.3791/4159 ◽  
2012 ◽  
Author(s):  
Alimatou M. Tchafa ◽  
Arpit D. Shah ◽  
Shafei Wang ◽  
Melissa T. Duong ◽  
Adrian C. Shieh
Keyword(s):  
Cell Culture ◽  
Fluid Flow ◽  
Cell Invasion ◽  
Interstitial Fluid ◽  
Three Dimensional ◽  
Tumor Cell Invasion ◽  
Cell Culture Model ◽  
Interstitial Fluid Flow ◽  
Culture Model ◽  
Dimensional Cell

A Three-dimensional Cell Culture Model for Bovine Endometrium: Regeneration of a Multicellular Spheroid Using Ascorbate

Placenta ◽  
2003 ◽  
Vol 24 (2-3) ◽  
pp. 258-269 ◽  
Author(s):  
N Yamauchi ◽  
O Yamada ◽  
T Takahashi ◽  
K Imai ◽  
T Sato ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Multicellular Spheroid ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell

Cytocompatibility of Biodentine using a three-dimensional cell culture model

2015 ◽  
Vol 49 (6) ◽  
pp. 574-580 ◽  
Author(s):  
E. J. N. L. Silva ◽  
P. M. Senna ◽  
G. De-Deus ◽  
A. A. Zaia
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell

scholarly journals Cytotoxicity Profile of Endodontic Sealers Provided by 3D Cell Culture Experimental Model

2016 ◽  
Vol 27 (6) ◽  
pp. 652-656 ◽  
Author(s):  
Emmanuel João Nogueira Leal Silva ◽  
Nancy Kudsi de Carvalho ◽  
Carina Taboada Ronconi ◽  
Gustavo De-Deus ◽  
Mario Luis Zuolo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Type I ◽  
Cell Culture Model ◽  
Cytotoxic Effects ◽  
Culture Model ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Dimensional Cell

Abstract The aim of the present study was to evaluate the cytotoxic effects of five endodontic sealers (AH Plus, Endomethasone N, EndoSequence BC, MTA Fillapex and Pulp Canal Sealer EWT) using a three-dimensional (3D) cell culture model. A conventional bi-dimensional (2D) cell culture model was used as reference technique for comparison. Balb/c 3T3 fibroblasts were cultured in conventional bi-dimensional cell culture and in rat-tail collagen type I three-dimensional cell culture models. Then, both cell cultures were incubated with elutes of freshly mixed endodontic sealers for 24 h. Cell viability was measured by the methyl-thiazol-diphenyltetrazolium assay (MTT). Data were statistically analyzed using ANOVA and the Tukey test at a significance level of p<0.05. All tested sealers exhibited cytotoxic effects; however, cytotoxic effect was culture model- and sealer-dependent. Sealers showed higher cytotoxicity in 2D than in 3D cell culture model (p<0.05). In both conditions, EndoSequence BC showed the lowest cytotoxicity (p<0.05). MTA Fillapex was much more cytotoxic than the other tested endodontic sealers (p<0.05), with the exception of AH Plus in the 2D cell culture model (p>0.05). Endomethasone N and Pulp Canal Sealer EWT showed lower cytotoxic effects than AH Plus in 2D cell culture model (p<0.05); however no statistical differences was observed among these sealers in 3D cell culture model. It may be concluded that cytotoxicity was higher in 2D cell culture compared to 3D cell culture. EndoSequence BC sealer exhibited the highest cytocompatibility and MTA Fillapex the lowest cytocompatibility.

scholarly journals Mifepristone, but not levonorgestrel, inhibits human blastocyst attachment to an in vitro endometrial three-dimensional cell culture model

2007 ◽  
Vol 22 (11) ◽  
pp. 3031-3037 ◽  
Author(s):  
P.G.L. Lalitkumar ◽  
S. Lalitkumar ◽  
C.X. Meng ◽  
A. Stavreus-Evers ◽  
F. Hambiliki ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Human Blastocyst ◽  
Culture Model ◽  
Dimensional Cell

Three-Dimensional Cell Culture Model Utilization in Renal Carcinoma Cancer Stem Cell Research

2018 ◽  
pp. 47-66 ◽  
Author(s):  
Kamila Maliszewska-Olejniczak ◽  
Klaudia K. Brodaczewska ◽  
Zofia F. Bielecka ◽  
Anna M. Czarnecka
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Stem Cell Research ◽  
Renal Carcinoma ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell

Cytocompatibility of calcium silicate-based sealers in a three-dimensional cell culture model

2016 ◽  
Vol 21 (5) ◽  
pp. 1531-1536 ◽  
Author(s):  
Emmanuel João Nogueira Leal da Silva ◽  
Alexandre A. Zaia ◽  
Ove A. Peters
Keyword(s):  
Cell Culture ◽  
Calcium Silicate ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell

A novel three-dimensional cell culture model based on the composite dacron/collagen scaffold for prostate cancer

2019 ◽  
Vol 18 (1) ◽  
pp. e1152-e1153
Author(s):  
M. Hu ◽  
X. Liu ◽  
C. Li ◽  
Q. Ding ◽  
L. Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Three Dimensional ◽  
Collagen Scaffold ◽  
Cell Culture Model ◽  
Model Based ◽  
Culture Model ◽  
Dimensional Cell

scholarly journals Characterization of an Aggregated Three-Dimensional Cell Culture Model by Multimodal Mass Spectrometry Imaging

2020 ◽  
Vol 92 (18) ◽  
pp. 12538-12547 ◽  
Author(s):  
Lucy E. Flint ◽  
Gregory Hamm ◽  
Joseph D. Ready ◽  
Stephanie Ling ◽  
Catherine J. Duckett ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Mass Spectrometry Imaging ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell
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