scholarly journals Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

10.3791/53942 ◽  
2016 ◽  
Author(s):  
Milad Nazari ◽  
Mark T. Bokhart ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Body Mass ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Whole Body ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

2019 ◽  
Vol 11 (46) ◽  
pp. 5929-5938 ◽  
Author(s):  
Sitora Khodjaniyazova ◽  
Nicholas J. Hanne ◽  
Jacqueline H. Cole ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Direct Analysis ◽  
Chemical Treatments ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Direct analysis and IR-MALDESI mass spectrometry imaging of fresh mouse bones that underwent no chemical treatments other than flash-freezing.

scholarly journals Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 810
Author(s):  
Ying Xi ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Rat Liver ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Ionization Mode ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Mass spectrometry imaging is a powerful tool to analyze a large number of metabolites with their spatial coordinates collected throughout the sample. However, the significant differences in ionization efficiency pose a big challenge to metabolomic mass spectrometry imaging. To solve the challenge and obtain a complete data profile, researchers typically perform experiments in both positive and negative ionization modes, which is time-consuming. In this work, we evaluated the use of the dicationic reagent, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride (abbreviated to [C5(bpyr)2]F2) to detect a broad range of metabolites in the positive ionization mode by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging (IR-MALDESI MSI). [C5(bpyr)2]F2 at 10 µM was doped in 50% MeOH/H2O (v/v) electrospray solvent to form +1 charged adducted ions with anionic species (−1 charged) through post-electrospray ionization. This method was demonstrated with sectioned rat liver and hen ovary. A total of 73 deprotonated metabolites from rat liver tissue sections were successfully adducted with [C5(bpyr)2]2+ and putatively identified in the adducted positive ionization polarity, along with 164 positively charged metabolite ions commonly seen in positive ionization mode, which resulted in 44% increased molecular coverage. In addition, we were able to generate images of hen ovary sections showing their morphological features. Following-up tandem mass spectrometry (MS/MS) indicated that this dicationic reagent [C5(bpyr)2]2+ could form ionic bonds with the headgroup of glycerophospholipid ions. The addition of the dicationic reagent [C5(bpyr)2]2+ in the electrospray solvent provides a rapid and effective way to enhance the detection of metabolites in positive ionization mode.

scholarly journals Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 654-661 ◽  
Author(s):  
Milad Nazari ◽  
Mark T. Bokhart ◽  
Philip L. Loziuk ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

IR-MALDESI quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissues.

scholarly journals Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging analysis of endogenous metabolites in cherry tomatoes

The Analyst ◽  
2020 ◽  
Vol 145 (16) ◽  
pp. 5516-5523
Author(s):  
M. Caleb Bagley ◽  
Crystal L. Pace ◽  
Måns Ekelöf ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Spatially Resolved ◽  
Endogenous Metabolites ◽  
Cherry Tomatoes ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

We report the spatially resolved metabolic profiling of cherry tomatoes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI); an ambient mass spectrometry imaging (MSI) technique that requires no sample derivatization.

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