scholarly journals Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation

10.3791/55495 ◽  
2017 ◽  
Author(s):  
Rupesh Agrawal ◽  
Praveen Kumar Balne ◽  
Sai Bo Bo Tun ◽  
Yeo Sia Wey ◽  
Neha Khandelwal ◽  
...  
Keyword(s):  
Fluorescent Dye ◽  
Flow Dynamics ◽  
Retinal Circulation ◽  
Dye Labeling

scholarly journals Fluorescent Dye Labeling Changes the Biodistribution of Tumor-Targeted Nanoparticles

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1004
Author(s):  
Patricia Álamo ◽  
Victor Pallarès ◽  
María Virtudes Céspedes ◽  
Aïda Falgàs ◽  
Julieta M. Sanchez ◽  
...  
Keyword(s):  
Fluorescent Dye ◽  
Target Tissue ◽  
Dye Molecules ◽  
Targeted Nanoparticles ◽  
Self Assembling ◽  
Target Organs ◽  
Common Strategy ◽  
Uptake Studies ◽  
Living Organisms ◽  
Dye Labeling

Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, to which extent the labeling processes can alter the original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent dye molecules, namely, ATTO488 (ATTO) and Sulfo-Cy5 (S-Cy5), have been covalently attached to a well-characterized CXCR4-targeted self-assembling protein nanoparticle (known as T22-GFP-H6). The biodistribution of labeled T22-GFP-H6-ATTO and T22-GFP-H6-S-Cy5 nanoparticles has been then compared to that of the non-labeled nanoparticle in different CXCR4+ tumor mouse models. We observed that while parental T22-GFP-H6 nanoparticles accumulated mostly and specifically in CXCR4+ tumor cells, labeled T22-GFP-H6-ATTO and T22-GFP-H6-S-Cy5 nanoparticles showed a dramatic change in the biodistribution pattern, accumulating in non-target organs such as liver or kidney while reducing tumor targeting capacity. Therefore, the use of such labeling molecules should be avoided in target and non-target tissue uptake studies during the design and development of targeted nanoscale drug delivery systems, since their effect over the fate of the nanomaterial can lead to considerable miss-interpretations of the actual nanoparticle biodistribution.

Protein Fluorescent Dye Labeling

2013 ◽  
pp. 2015-2015
Keyword(s):  
Fluorescent Dye ◽  
Dye Labeling

scholarly journals A new tool to ensure the fluorescent dye labeling stability of nanocarriers: A real challenge for fluorescence imaging

2013 ◽  
Vol 170 (3) ◽  
pp. 334-342 ◽  
Author(s):  
Guillaume Bastiat ◽  
Christian Oliver Pritz ◽  
Clemens Roider ◽  
Florian Fouchet ◽  
Erwann Lignières ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Dye ◽  
Real Challenge ◽  
Dye Labeling

3-Dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 164-165
Author(s):  
Thomas J. Deerinck ◽  
Maryann E. Martone ◽  
Varda Lev-Ram ◽  
David P. L. Green ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Reactive Oxygen ◽  
Confocal Laser Scanning Microscope ◽  
Fluorescent Dye ◽  
Confocal Laser ◽  
3 Dimensional ◽  
Voltage Electron ◽  
Dimensional Distribution ◽  
Electron Microscopes ◽  
New Generation

The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.

The response of the retinal circulation to altitude

1971 ◽  
Vol 127 (4) ◽  
pp. 708-711 ◽  
Author(s):  
R. Frayser
Keyword(s):  
Retinal Circulation

The role of the sinuses of valsalva in aortic root flow dynamics and aortic root surgery

2007 ◽  
Vol 55 (S 1) ◽  
Author(s):  
F Schoenhoff ◽  
C Loupatatzis ◽  
FS Eckstein ◽  
C Stoupis ◽  
FF Immer ◽  
...  
Keyword(s):  
Aortic Root ◽  
Flow Dynamics ◽  
Aortic Root Surgery

Retinal circulation times in branch retinal vein occlusion

1995 ◽  
Vol 9 (2) ◽  
pp. 107 ◽  
Author(s):  
Gumin Kang ◽  
Jaeheung Lee
Keyword(s):  
Retinal Vein Occlusion ◽  
Branch Retinal Vein Occlusion ◽  
Retinal Circulation ◽  
Vein Occlusion

Use of a Topical Fluorescent Dye to Evaluate Effectiveness of Sunscreen Application

1998 ◽  
Vol 134 (4) ◽  
pp. 515-517 ◽  
Author(s):  
M. D. Gaughan
Keyword(s):  
Fluorescent Dye

Numerical Simulation of Blood Flow Dynamics using FEM

2018 ◽  
Author(s):  
LEANDRO MARQUES
Keyword(s):  
Numerical Simulation ◽  
Blood Flow ◽  
Flow Dynamics ◽  
Blood Flow Dynamics
Sign in / Sign up

Export Citation Format

Share Document