scholarly journals Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation

10.3791/56245 ◽  
2017 ◽  
Author(s):  
Ben Shirley ◽  
Yanxin Li ◽  
Joan H.M. Knoll ◽  
Peter K. Rogan
Keyword(s):  
Dicentric Chromosome ◽  
Dose Estimation ◽  
Chromosome Identification ◽  
Radiation Biodosimetry

scholarly journals Automated Cytogenetic Biodosimetry at Population-Scale

Radiation ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 79-94
Author(s):  
Peter K. Rogan ◽  
Eliseos J. Mucaki ◽  
Ben C. Shirley ◽  
Yanxin Li ◽  
Ruth C. Wilkins ◽  
...  
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Dicentric Chromosome ◽  
Automated Assignment ◽  
Simulated Population ◽  
Dose Estimation ◽  
Processing Times ◽  
Multiple Processors ◽  
Exposure Levels ◽  
Population Scale

The dicentric chromosome (DC) assay accurately quantifies exposure to radiation; however, manual and semi-automated assignment of DCs has limited its use for a potential large-scale radiation incident. The Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) software automates unattended DC detection and determines radiation exposures, fulfilling IAEA criteria for triage biodosimetry. This study evaluates the throughput of high-performance ADCI (ADCI-HT) to stratify exposures of populations in 15 simulated population scale radiation exposures. ADCI-HT streamlines dose estimation using a supercomputer by optimal hierarchical scheduling of DC detection for varying numbers of samples and metaphase cell images in parallel on multiple processors. We evaluated processing times and accuracy of estimated exposures across census-defined populations. Image processing of 1744 samples on 16,384 CPUs required 1 h 11 min 23 s and radiation dose estimation based on DC frequencies required 32 sec. Processing of 40,000 samples at 10 exposures from five laboratories required 25 h and met IAEA criteria (dose estimates were within 0.5 Gy; median = 0.07). Geostatistically interpolated radiation exposure contours of simulated nuclear incidents were defined by samples exposed to clinically relevant exposure levels (1 and 2 Gy). Analysis of all exposed individuals with ADCI-HT required 0.6–7.4 days, depending on the population density of the simulation.

scholarly journals RENEB/EURADOS Field Exercise 2019: Robust Dose Estimation under outdoor Conditions based on the Dicentric Chromosome Assay

2021 ◽  
pp. 1-48
Author(s):  
David Endesfelder ◽  
Ursula Oestreicher ◽  
Ulrike Kulka ◽  
Elizabeth A. Ainsbury ◽  
Jayne Moquet ◽  
...  
Keyword(s):  
Dicentric Chromosome ◽  
Dose Estimation ◽  
Field Exercise

scholarly journals RADIATION DOSE ESTIMATION BY COMPLETELY AUTOMATED INTERPRETATION OF THE DICENTRIC CHROMOSOME ASSAY

2019 ◽  
Author(s):  
Yanxin Li ◽  
Ben C Shirley ◽  
Ruth C Wilkins ◽  
Farrah Norton ◽  
Joan H M Knoll ◽  
...  
Keyword(s):  
Radiation Dose ◽  
Dicentric Chromosome ◽  
Dose Estimation ◽  
Automated Interpretation

QUICKSCAN DICENTRIC CHROMOSOME ANALYSIS FOR RADIATION BIODOSIMETRY

2010 ◽  
Vol 98 (2) ◽  
pp. 276-281 ◽  
Author(s):  
F N. Flegal ◽  
Y Devantier ◽  
J P. McNamee ◽  
R C. Wilkins
Keyword(s):  
Chromosome Analysis ◽  
Dicentric Chromosome ◽  
Radiation Biodosimetry

Dose Estimation Using Dicentric Chromosome Assay and Cytokinesis Block Micronucleus Assay

2014 ◽  
Vol 106 (6) ◽  
pp. 787-797 ◽  
Author(s):  
Andrea De Amicis ◽  
Stefania De Sanctis ◽  
Sara Di Cristofaro ◽  
Valeria Franchini ◽  
Elisa Regalbuto ◽  
...  
Keyword(s):  
Micronucleus Assay ◽  
Dicentric Chromosome ◽  
Dose Estimation

Interlaboratory Comparison of the Dicentric Chromosome Assay for Radiation Biodosimetry in Mass Casualty Events

2008 ◽  
Vol 169 (5) ◽  
pp. 551-560 ◽  
Author(s):  
Ruth C. Wilkins ◽  
Horst Romm ◽  
Tzu-Cheg Kao ◽  
Akio A. Awa ◽  
Mitsuaki A. Yoshida ◽  
...  
Keyword(s):  
Interlaboratory Comparison ◽  
Mass Casualty ◽  
Dicentric Chromosome ◽  
Mass Casualty Events ◽  
Radiation Biodosimetry

scholarly journals Study of Solving the Bottleneck Problem of the Estimation of Biological Dose in the Window of Time of Medical Emergency in Large-scale Nuclear Radiation Accidents

2020 ◽  
Author(s):  
Gang Liu
Keyword(s):  
Dose Rate ◽  
Irradiation Dose ◽  
Large Scale ◽  
Peripheral Blood Lymphocytes ◽  
Nuclear Radiation ◽  
Dicentric Chromosome ◽  
Medical Emergency ◽  
Dose Estimation ◽  
Standard Curve ◽  
Biological Dose

Abstract Purpose In order to achieve the goal of rapid response, effective disposal and protection of life of large-scale radiation events, how to establish the uniform standard curve of biological dose estimation for chromosome aberration analysis becomes an urgent need. Methods Chromosomal aberrations with different irradiation dose rates were used to analyze the biological dose curve and the share of the “dicentric + ring” caused by the dose rate at each dose point. The dose-rate effect of 60Co-rays on peripheral blood lymphocytes was analyzed by statistical method . Results Irradiation dose is dominant ; At each dose point, “(dicentric chromosome + centric rings) /cell” is proportional to "dose rate", that is, Y = k X + b;Between 1-5Gy dose, “(dicentric chromosome + centric rings) /Cell ” holds a quadratic linear relationship with dose rate, that is,y = ax2 + bx + c. Conclusion The fraction of “dicentric + ring” caused by dose rate was calculated, if “Dose rate” is Z Gy•min− 1, it corresponds to an increase in linear relationships. Biological dose estimation curve :Y = 3.318 × 10− 3+2.0541 × 10− 2 x + 7.1721 × 10− 2x2(1.16Gy•min− 1༛R2 = 0.9997)(3).Dose rate :Y Dose rate = 1.2534 × 10− 2 x 2+6.6164 × 10− 2 x -2.732 × 10− 3༈0.01Gy•min− 1༛R2 = 0.999༉(1).The estimated dose is formula (3) – (1.16-Z) × formula (1).

DOSE ESTIMATION SOFTWARE FOR RADIATION BIODOSIMETRY

2010 ◽  
Vol 98 (2) ◽  
pp. 290-295 ◽  
Author(s):  
Elizabeth A. Ainsbury ◽  
David C. Lloyd
Keyword(s):  
Dose Estimation ◽  
Radiation Biodosimetry

scholarly journals Validation of the dicentric chromosome assay for radiation biological dosimetry in South Korea

2019 ◽  
Vol 60 (5) ◽  
pp. 555-563
Author(s):  
Younghyun Lee ◽  
Young Woo Jin ◽  
Ruth C Wilkins ◽  
Seongjae Jang
Keyword(s):  
South Korea ◽  
Irradiation Dose ◽  
Dicentric Chromosome ◽  
Medical Sciences ◽  
Radiation Accidents ◽  
Medical Response ◽  
Intercomparison Exercises ◽  
And Performance ◽  
Health Canada ◽  
Radiation Biodosimetry

Abstract The dicentric chromosome assay (DCA) is a well-established biodosimetry test to estimate exposure to ionizing radiation. The Korea Institute of Radiological and Medical Sciences (KIRAMS) established a DCA protocol as a medical response to radiation emergencies in South Korea. To maintain its accuracy and performance, intercomparison exercises with Health Canada (HC) have been conducted; herein, we aimed to validate our capacity of DCA analysis based on those results. Blood samples irradiated at HC were shipped to KIRAMS to assess the irradiation dose to blinded samples using conventional DCA full scoring and triage-based techniques (conventional DCA scoring in triage mode and DCA QuickScan method). Actual doses fell within the 95% confidence intervals of dose estimates for 70–100% of the blinded samples in 2015–2018. All methods discriminated binary dose categories, reflecting clinical significance. This DCA can be used as a reliable radiation biodosimetry tool in preparation for radiation accidents in South Korea.

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