scholarly journals Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

10.3791/56345 ◽  
2018 ◽  
Author(s):  
Nathalie Dinguirard ◽  
Codie Heinemann ◽  
Timothy P. Yoshino
Keyword(s):  
Schistosoma Mansoni ◽  
Human Blood ◽  
Blood Fluke ◽  
Mass Isolation

scholarly journals In vitro and in silico analysis of signal peptides from the human blood fluke,Schistosoma mansoni

2005 ◽  
Vol 45 (2) ◽  
pp. 201-211 ◽  
Author(s):  
Mark S. Pearson ◽  
Donald P. McManus ◽  
Danielle J. Smyth ◽  
Fred A. Lewis ◽  
Alex Loukas
Keyword(s):  
Schistosoma Mansoni ◽  
Human Blood ◽  
In Silico ◽  
In Silico Analysis ◽  
Signal Peptides ◽  
Blood Fluke ◽  
Silico Analysis

Corrigendum to “The fugitive LTR retrotransposon from the genome of the human blood fluke, Schistosoma mansoni” [International Journal for Parasitology 34 (2004) 1365–1375]

2005 ◽  
Vol 35 (4) ◽  
pp. 461
Author(s):  
T. Laha ◽  
A. Loukas ◽  
D.J. Smyth ◽  
C.S. Copeland ◽  
P.J. Brindley
Keyword(s):  
Schistosoma Mansoni ◽  
Human Blood ◽  
Ltr Retrotransposon ◽  
Blood Fluke

scholarly journals Solution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood Fluke Schistosoma mansoni

2014 ◽  
Vol 289 (10) ◽  
pp. 7151-7163 ◽  
Author(s):  
Xinying Jia ◽  
Leigh Schulte ◽  
Alex Loukas ◽  
Darren Pickering ◽  
Mark Pearson ◽  
...  
Keyword(s):  
Protein Binding ◽  
Schistosoma Mansoni ◽  
Human Blood ◽  
Solution Structure ◽  
Vaccine Antigen ◽  
Membrane Interactions ◽  
Blood Fluke ◽  
Binding Partners

scholarly journals Immunoreactivity to the pancreatic polypeptide family in the nervous system of the adult human blood fluke, Schistosoma mansoni

1991 ◽  
Vol 263 (1) ◽  
pp. 199-199
Author(s):  
P. J. Skuce ◽  
C. F. Johnston ◽  
I. Fairweather ◽  
D. W. Halton ◽  
C. Shaw ◽  
...  
Keyword(s):  
Nervous System ◽  
Schistosoma Mansoni ◽  
Pancreatic Polypeptide ◽  
Human Blood ◽  
Blood Fluke ◽  
Adult Human

Lectin and Human Blood Group Determinants of Schistosoma mansoni: Alteration Following In vitro Transformation of Miracidium to Mother Sporocyst

1977 ◽  
Vol 63 (5) ◽  
pp. 818 ◽  
Author(s):  
Timothy P. Yoshino ◽  
Thomas C. Cheng ◽  
Lothar R. Renwrantz
Keyword(s):  
Schistosoma Mansoni ◽  
Blood Group ◽  
Human Blood ◽  
Human Blood Group ◽  
In Vitro Transformation ◽  
Mother Sporocyst

scholarly journals Characterization of a female-specific cDNA derived from a developmentally regulated mRNA in the human blood fluke Schistosoma mansoni.

1986 ◽  
Vol 83 (15) ◽  
pp. 5544-5548 ◽  
Author(s):  
L. Bobek ◽  
D. M. Rekosh ◽  
H. van Keulen ◽  
P. T. LoVerde
Keyword(s):  
Schistosoma Mansoni ◽  
Human Blood ◽  
Developmentally Regulated ◽  
Blood Fluke ◽  
Female Specific

scholarly journals Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni

2021 ◽  
Author(s):  
Chrystelle Lasica ◽  
Ronaldo de Carvalho Augusto ◽  
Helene Mone ◽  
Gabriel Mouahid ◽  
Cristian Chaparro ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Human Blood ◽  
Chromatin Immunoprecipitation ◽  
Biological Material ◽  
Blood Fluke ◽  
Human Parasite

Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq. Using the human parasite Schistosoma mansoni, whose production requires scarifying animals and should therefore kept to a minimum, we show here that the automated ChIPmentation is suitable for limited biological material. We define as operational limit ≥20,000 cells. We also present a streamlined protocol for the preparation of ChIP input libraries.

scholarly journals Therapeutic Effect of Diminazene Aceturate on Parasitic Blood Fluke Schistosoma mansoni Infection

2020 ◽  
Vol 64 (11) ◽  
Author(s):  
Mariana G. de Brito ◽  
Ana C. Mengarda ◽  
George L. Oliveira ◽  
Maria E. Cirino ◽  
Tais C. Silva ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Chronic Infection ◽  
Worm Burden ◽  
Egg Production ◽  
Laser Scanning Microscopy ◽  
Early Infection ◽  
Blood Fluke ◽  
Serum Aminotransferase ◽  
Content Type

ABSTRACT Praziquantel is currently the only drug available to treat schistosomiasis, a disease of enormous public health significance caused by a blood fluke of the genus Schistosoma. Diminazene, a drug approved by the FDA, has been successfully used to treat diseases caused by blood protozoan parasites. In this study, we evaluated the antiparasitic properties of diminazene against Schistosoma mansoni ex vivo and in mice harboring either chronic or early S. mansoni infections. In vitro, we monitored phenotypic and tegumental changes as well as the effects of the drug on pairing and egg production. In mice infected with either adult (chronic infection) or immature (early infection) worms, diminazene was administered intraperitoneally (10 to 100 mg/kg of body weight) or by oral gavage (100 to 400 mg/kg), and we studied the influence of the drug on worm burden and egg production. Liver and spleen pathologies and serum aminotransferase levels were also analyzed. In vitro, 50% effective concentrations (EC50) and EC90 revealed that diminazene is able to kill both immature and adult parasites, and its effect was time and concentration dependent. In addition, confocal laser scanning microscopy showed morphological alterations in the teguments of schistosomes. In an animal model, the influence of the drug on worm burden, egg production, hepatomegaly, and splenomegaly depended on the dosing regimen applied and the route of administration. Diminazene also caused a significant reduction in aminotransferase levels. Comparatively, diminazene treatment was more effective in chronic infection than in early infection. In tandem, our study revealed that diminazene possesses anthelmintic properties and inhibits liver injury caused by Schistosoma eggs.

scholarly journals Genomic linkage map of the human blood fluke Schistosoma mansoni

2009 ◽  
Vol 10 (6) ◽  
pp. R71 ◽  
Author(s):  
Charles D Criscione ◽  
Claudia LL Valentim ◽  
Hirohisa Hirai ◽  
Philip T LoVerde ◽  
Timothy JC Anderson
Keyword(s):  
Schistosoma Mansoni ◽  
Linkage Map ◽  
Human Blood ◽  
Blood Fluke ◽  
Genomic Linkage
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