scholarly journals Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

10.3791/56614 ◽  
2018 ◽  
Author(s):  
Piotr Pander ◽  
Przemyslaw Data ◽  
Fernando B. Dias
Keyword(s):  
Organic Compounds ◽  
Iccd Camera ◽  
Time Resolved ◽  
Free Environment ◽  
Triplet Harvesting

Time-resolved characterization of organic compounds in PM2.5 collected at Oki Island, Japan, affected by transboundary pollution of biomass and non-biomass burning from Northeast China

2021 ◽  
Vol 750 ◽  
pp. 142183
Author(s):  
Fumikazu Ikemori ◽  
Katsushige Uranishi ◽  
Takahiro Sato ◽  
Makoto Fujihara ◽  
Hitomi Hasegawa ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Biomass Burning ◽  
Northeast China ◽  
Transboundary Pollution ◽  
Time Resolved

Characterization of Organic Compounds in Aframomum melegueta K. Schum Using GC-MS

2019 ◽  
Vol 08 (02) ◽  
Author(s):  
Oladunmoye MK
Keyword(s):  
Organic Compounds

Characterization of Soluble Organic Compounds and Complexation of Copper, Nickel, and Zinc in Extracts of Sludge-amended Soils

1987 ◽  
Vol 16 (4) ◽  
pp. 341-348 ◽  
Author(s):  
L. M. Dudley ◽  
B. L. McNeal ◽  
J. E. Baham ◽  
C. S. Coray ◽  
H. H. Cheng
Keyword(s):  
Organic Compounds ◽  
Amended Soils ◽  
Copper Nickel

Methods for characterization of organic compounds in atmospheric aerosol particles

2014 ◽  
Vol 407 (20) ◽  
pp. 5877-5897 ◽  
Author(s):  
Jevgeni Parshintsev ◽  
Tuulia Hyötyläinen
Keyword(s):  
Organic Compounds ◽  
Atmospheric Aerosol ◽  
Aerosol Particles ◽  
Atmospheric Aerosol Particles

scholarly journals Characterization of a Novel FRET Probe in a Crowded Environment using Time-Resolved Anisotropy

2017 ◽  
Vol 112 (3) ◽  
pp. 346a
Author(s):  
Hannah Leopold ◽  
Megan Currie ◽  
Jacob Schwarz ◽  
Arnold J. Boersma ◽  
Erin D. Sheets ◽  
...  
Keyword(s):  
Time Resolved ◽  
Crowded Environment

scholarly journals Halogenated Volatile Organic Compounds in Water Samples and Inorganic Elements Levels in Ores for Characterizing a High Anthropogenic Polluted Area in the Northern Latium Region (Italy)

2021 ◽  
Vol 18 (4) ◽  
pp. 1628
Author(s):  
Mario Vincenzo Russo ◽  
Ivan Notardonato ◽  
Alberto Rosada ◽  
Giuseppe Ianiri ◽  
Pasquale Avino
Keyword(s):  
Organic Compounds ◽  
Mean Value ◽  
Greenhouse Crops ◽  
River Waters ◽  
Liquid Liquid Extraction ◽  
Volatile Organic ◽  
High Concentrations ◽  
Very High ◽  
Inorganic Fraction

This paper shows a characterization of the organic and inorganic fraction of river waters (Tiber and Marta) and ores/soil samples collected in the Northern Latium region of Italy for evaluating the anthropogenic/natural source contribution to the environmental pollution of this area. For organic compounds, organochloride volatile compounds in Tiber and Marta rivers were analyzed by two different clean-up methods (i.e., liquid–liquid extraction and static headspace) followed by gas chromatography–electron capture detector (GC-ECD) analysis. The results show very high concentrations of bromoform (up to 1.82 and 3.2 µg L−1 in Tiber and Marta rivers, respectively), due to the presence of greenhouse crops, and of chloroform and tetrachloroethene, due to the presence of handicrafts installations. For the qualitative and quantitative assessment of the inorganic fraction, it is highlighted the use of a nuclear analytical method, instrumental neutron activation analysis, which allows having more information as possible from the sample without performing any chemical-physical pretreatment. The results have evidenced high levels of mercury (mean value 88.6 µg g−1), antimony (77.7 µg g−1), strontium (12,039 µg g−1) and zinc (103 µg g−1), whereas rare earth elements show levels similar to the literature data. Particular consideration is drawn for arsenic (414 µg g−1): the levels found in this paper (ranging between 1 and 5100 µg g−1) explain the high content of such element (as arsenates) in the aquifer, a big issue in this area.

scholarly journals KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements

2021 ◽  
Author(s):  
Diana Spiegelberg ◽  
Jonas Stenberg ◽  
Pascale Richalet ◽  
Marc Vanhove
Keyword(s):  
Flow Cytometry ◽  
Live Cells ◽  
Time Resolved ◽  
Surface Receptors ◽  
Full Characterization ◽  
End Point ◽  
Titration Curves ◽  
Kinetics Of

AbstractDesign of next-generation therapeutics comes with new challenges and emulates technology and methods to meet them. Characterizing the binding of either natural ligands or therapeutic proteins to cell-surface receptors, for which relevant recombinant versions may not exist, represents one of these challenges. Here we report the characterization of the interaction of five different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate target receptors using LigandTracer. The method offers the advantage of being performed on live cells, alleviating the need for a recombinant source of the receptor. Furthermore, time-resolved measurements, in addition to allowing the determination of the affinity of the studied drug to its target, give access to the binding kinetics thereby providing a full characterization of the system. In this study, we also compared time-resolved LigandTracer data with end-point KD determination from flow cytometry experiments and hypothesize that discrepancies between these two approaches, when they exist, generally come from flow cytometry titration curves being acquired prior to full equilibration of the system. Our data, however, show that knowledge of the kinetics of the interaction allows to reconcile the data obtained by flow cytometry and LigandTracer and demonstrate the complementarity of these two methods.

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