scholarly journals Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

10.3791/57015 ◽  
2018 ◽  
Author(s):  
Virginie Sabado ◽  
Emi Nagoshi
Keyword(s):  
Single Cell ◽  
Live Imaging ◽  
Circadian Clocks ◽  
Larval Brain ◽  
Brain Culture

scholarly journals Quantitative single-cell live imaging links HES5 dynamics with cell-state and fate in murine neurogenesis

2018 ◽  
Author(s):  
Cerys S Manning ◽  
Veronica Biga ◽  
James Boyd ◽  
Jochen Kursawe ◽  
Bodvar Ymisson ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Fate ◽  
Live Imaging ◽  
Protein Quantification ◽  
Expression Change ◽  
Dynamic View ◽  
Transient Period ◽  
Gene Expression Dynamics ◽  
Transcription Factor Expression ◽  
Protein Fluctuations

AbstractDuring embryogenesis cells make fate decisions within complex tissue environments. The levels and dynamics of transcription factor expression regulate these decisions. Here we use single cell live imaging of an endogenous HES5 reporter and absolute protein quantification to gain a dynamic view of neurogenesis in the embryonic mammalian spinal cord. We report that dividing neural progenitors show both aperiodic and periodic HES5 protein fluctuations. Mathematical modelling suggests that in progenitor cells the HES5 oscillator operates close to its bifurcation boundary where stochastic conversions between dynamics are possible. HES5 expression becomes more frequently periodic as cells transition to differentiation which, coupled with an overall decline in HES5 expression, creates a transient period of oscillations with higher fold expression change. This increases the decoding capacity of HES5 oscillations and correlates with interneuron versus motor neuron cell fate. Thus, HES5 undergoes complex changes in gene expression dynamics as cells differentiate.

Abstract LB-119: Single-cell live imaging of paclitaxel-resistant cancer cell generation

2016 ◽  
Author(s):  
Na Niu ◽  
Jie Zhang ◽  
Ning Zhang ◽  
Imelda Mercado-Uribe ◽  
Fangfang Tao ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cell ◽  
Live Imaging ◽  
Cell Generation

Su1736 Live Imaging of Single Cell Reveals Single Stem Cell Dynamics in an Organoid Derived From Murine Small Intestine

2013 ◽  
Vol 144 (5) ◽  
pp. S-464
Author(s):  
Nobukatsu Horita ◽  
Kiichiro Tsuchiya ◽  
Shuji Hibiya ◽  
Keita Fukushima ◽  
Yoshihito Kano ◽  
...  
Keyword(s):  
Small Intestine ◽  
Stem Cell ◽  
Single Cell ◽  
Live Imaging ◽  
Cell Dynamics ◽  
Murine Small Intestine

scholarly journals The Single-Cell Yin and Yang of Live Imaging and Transcriptomics

Cell Systems ◽  
2017 ◽  
Vol 4 (4) ◽  
pp. 375-377
Author(s):  
Michael Tsabar ◽  
Galit Lahav
Keyword(s):  
Single Cell ◽  
Live Imaging ◽  
Yin And Yang

scholarly journals A sensitive and bright single-cell resolution live imaging reporter of Wnt/ß-catenin signaling in the mouse

2010 ◽  
Vol 10 (1) ◽  
pp. 121 ◽  
Author(s):  
Anna Ferrer-Vaquer ◽  
Anna Piliszek ◽  
Guangnan Tian ◽  
Robert J Aho ◽  
Daniel Dufort ◽  
...  
Keyword(s):  
Single Cell ◽  
Live Imaging

scholarly journals Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

10.3791/56291 ◽  
2017 ◽  
Author(s):  
Rosa Gómez-Villafuertes ◽  
Lucía Paniagua-Herranz ◽  
Sergio Gascon ◽  
David de Agustín-Durán ◽  
María de la O Ferreras ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Biology ◽  
Cell Tracking ◽  
Live Imaging ◽  
Neural Populations

scholarly journals A bright single-cell resolution live imaging reporter of Notch signaling in the mouse

2013 ◽  
Vol 13 (1) ◽  
pp. 15 ◽  
Author(s):  
Sonja Nowotschin ◽  
Panagiotis Xenopoulos ◽  
Nadine Schrode ◽  
Anna-Katerina Hadjantonakis
Keyword(s):  
Notch Signaling ◽  
Single Cell ◽  
Live Imaging

scholarly journals Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for Lis1/Dynactin in Spindle Assembly and Mitotic Checkpoint Control

2005 ◽  
Vol 16 (11) ◽  
pp. 5127-5140 ◽  
Author(s):  
Karsten H. Siller ◽  
Madeline Serr ◽  
Ruth Steward ◽  
Tom S. Hays ◽  
Chris Q. Doe
Keyword(s):  
Cell Cycle ◽  
Fluorescent Protein ◽  
Spindle Assembly ◽  
Mitotic Checkpoint ◽  
Live Imaging ◽  
Cell Cycle Checkpoint ◽  
Checkpoint Control ◽  
Larval Brain ◽  
Independent Functions

Lis1 is required for nuclear migration in fungi, cell cycle progression in mammals, and the formation of a folded cerebral cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactin-dependent processes is not clearly understood. Here we generate and/or characterize mutants for Drosophila Lis1 and a dynactin subunit, Glued, to investigate the role of Lis1/dynactin in mitotic checkpoint function. In addition, we develop an improved time-lapse video microscopy technique that allows live imaging of GFP-Lis1, GFP-Rod checkpoint protein, green fluorescent protein (GFP)-labeled chromosomes, or GFP-labeled mitotic spindle dynamics in neuroblasts within whole larval brain explants. Our mutant analyses show that Lis1/dynactin have at least two independent functions during mitosis: first promoting centrosome separation and bipolar spindle assembly during prophase/prometaphase, and subsequently generating interkinetochore tension and transporting checkpoint proteins off kinetochores during metaphase, thus promoting timely anaphase onset. Furthermore, we show that Lis1/dynactin/dynein physically associate and colocalize on centrosomes, spindle MTs, and kinetochores, and that regulation of Lis1/dynactin kinetochore localization in Drosophila differs from both Caenorhabditis elegans and mammals. We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release.

scholarly journals Single-cell transcriptome profiling of the Ciona larval brain

2018 ◽  
Author(s):  
Sarthak Sharma ◽  
Wei Wang ◽  
Alberto Stolfi
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Expression Patterns ◽  
Transcriptome Profiling ◽  
Specific Gene ◽  
Larval Brain ◽  
Rnaseq Data ◽  
Cell Type Specific ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

AbstractThe tadpole-type larva of Ciona has emerged as an intriguing model system for the study of neurodevelopment. The Ciona intestinalis connectome has been recently mapped, revealing the smallest central nervous system (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent of larger CNS of vertebrates, sharing many conserved developmental processes, anatomical compartments, neuron subtypes, and even specific neural circuits. Thus, the Ciona tadpole offers a unique opportunity to understand the development and wiring of a chordate CNS at single-cell resolution. Here we report the use of single-cell RNAseq to profile the transcriptomes of single cells isolated by fluorescence-activated cell sorting (FACS) from the whole brain of Ciona robusta (formerly intestinalis Type A) larvae. We have also compared these profiles to bulk RNAseq data from specific subsets of brain cells isolated by FACS using cell type-specific reporter plasmid expression. Taken together, these datasets have begun to reveal the compartment- and cell-specific gene expression patterns that define the organization of the Ciona larval brain.

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