scholarly journals Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

10.3791/62431 ◽  
2021 ◽  
Author(s):  
Masashi Yamaguchi ◽  
Azusa Takahashi-Nakaguchi ◽  
Katsuyuki Uematsu ◽  
Masaki Taguchi ◽  
Michiyo Sato-Okamoto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Rapid Freezing

Bacterial envelope profiles revealed by freeze-substitution

1992 ◽  
Vol 50 (1) ◽  
pp. 868-869
Author(s):  
L.L. Graham ◽  
T.J. Beveridge
Keyword(s):  
Electron Microscopy ◽  
Freeze Substitution ◽  
Rapid Freezing ◽  
Chemical Fixation ◽  
Diverse Range ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Conventional Methods ◽  
Section Electron Microscopy ◽  
Better Than

Traditional methods of processing bacteria for thin section electron microscopy rely on chemical fixation and dehydration under conditions which maximize specimen deterioration. Cryotechniques, however, use physical fixation (rapid freezing) and are slowly being recognized as a superior alternative to the more conventional methods. Freeze-substitution is a cryotechnique which combines cryofixation with a gentle chemical fixation and dehydration regimen, yielding specimens amenable to standard embedment procedures and ultramicrotomy. Previous study has shown that freeze-substitution retains the molecular composition of eubacteria better than conventional methods of processing. In this study we extend our observations and show that a simple freeze-substitution protocol reliably preserves the ultrastructure of a diverse range of microorganisms including archaeobacteria and anaerobic eubacteria.

scholarly journals Rapid Freezing/Freeze Substitution Transmission Electron Microscopy Assessment of Cultured Endothelial Cell Glycocalyx Structure

2011 ◽  
Vol 17 (S2) ◽  
pp. 162-163
Author(s):  
E Ebong ◽  
F Macaluso ◽  
D Spray ◽  
J Tarbell
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endothelial Cell ◽  
Freeze Substitution ◽  
Rapid Freezing ◽  
Transmission Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

Rapid freezing and electron microscopy for the arrest of physiological processes

1974 ◽  
Vol 100 (2) ◽  
pp. 189-198 ◽  
Author(s):  
A. Harreveld ◽  
Janett Trubatch ◽  
Jana Steiner
Keyword(s):  
Electron Microscopy ◽  
Rapid Freezing ◽  
Physiological Processes
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