scholarly journals In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

10.3791/827 ◽  
2008 ◽  
Author(s):  
Mayumi Yamada ◽  
Phillip Yang
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Resonance Imaging

scholarly journals Magnetic resonance imaging tractography as a diagnostic tool in patients with spinal cord injury treated with human embryonic stem cells

2017 ◽  
Vol 30 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Geeta Shroff
Keyword(s):  
Magnetic Resonance Imaging ◽  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Resonance Imaging ◽  
Cord Injury

Introduction Spinal cord injury is a cause of severe disability and mortality. The pharmacological and non-pharmacological methods used, are unable to improve the quality of life in spinal cord injury. Spinal disorders have been treated with human embryonic stem cells. Magnetic resonance imaging and tractography were used as imaging modality to document the changes in the damaged cord, but the magnetic resonance imaging tractography was seen to be more sensitive in detecting the changes in the spinal cord. The present study was conducted to evaluate the diagnostic modality of magnetic resonance imaging tractography to determine the efficacy of human embryonic stem cells in chronic spinal cord injury. Materials and methods The study included the patients with spinal cord injury for whom magnetic resonance imaging tractography was performed before and after the therapy. Omniscan (gadodiamide) magnetic resonance imaging tractography was analyzed to assess the spinal defects and the improvement by human embryonic stem cell treatment. The patients were also scored by American Spinal Injury Association scale. Results Overall, 15 patients aged 15–44 years with clinical manifestations of spinal cord injury had magnetic resonance imaging tractography performed. The average treatment period was nine months. The majority of subjects ( n = 13) had American Spinal Injury Association score A, and two patients were at score C at the beginning of therapy. At the end of therapy, 10 patients were at score A, two patients were at score B and three patients were at score C. Improvements in patients were clearly understood through magnetic resonance imaging tractography as well as in clinical signs and symptoms. Conclusion Magnetic resonance imaging tractography can be a crucial diagnostic modality to assess the improvement in spinal cord injury patients.

scholarly journals Multimodal evaluation of in vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells

2008 ◽  
Vol 136 (4) ◽  
pp. 1028-1037.e1 ◽  
Author(s):  
Stephen L. Hendry ◽  
Koen E.A. van der Bogt ◽  
Ahmad Y. Sheikh ◽  
Takayasu Arai ◽  
Scott J. Dylla ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Resonance Imaging

In vivo detection limits of magnetically labeled embryonic stem cells in the rat brain using high-field (17.6 T) magnetic resonance imaging

NeuroImage ◽  
2005 ◽  
Vol 24 (3) ◽  
pp. 635-645 ◽  
Author(s):  
Albrecht Stroh ◽  
Cornelius Faber ◽  
Thomas Neuberger ◽  
Peer Lorenz ◽  
Katharina Sieland ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Rat Brain ◽  
High Field ◽  
Embryonic Stem ◽  
Resonance Imaging ◽  
In Vivo Detection

scholarly journals Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques

Stem Cells ◽  
2007 ◽  
Vol 25 (11) ◽  
pp. 2936-2944 ◽  
Author(s):  
Steven N. Ebert ◽  
David G. Taylor ◽  
Ha-Long Nguyen ◽  
David P. Kodack ◽  
Ronald J. Beyers ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Imaging Techniques ◽  
Embryonic Stem ◽  
Resonance Imaging ◽  
Magnetic Resonance Imaging Techniques

scholarly journals Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro

2021 ◽  
Author(s):  
Eun-Young Shin ◽  
Seah Park ◽  
Won Yun Choi ◽  
Dong Ryul Lee
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Male Hypogonadism ◽  
Testosterone Levels ◽  
Sequence Of Events ◽  
Short Period ◽  
Molecular Compounds

Abstract Background: Leydig cells (LCs) are testicular somatic cells that are the major producers of testosterone in males. Testosterone is essential for male physiology and reproduction. Reduced testosterone levels lead to hypogonadism and are associated with diverse pathologies, such as neuronal dysfunction, cardiovascular disease, and metabolic syndrome. LC transplantation is a promising therapy for hypogonadism; however, the number of LCs in the testis is very rare and they do not proliferate in vitro. Therefore, there is a need for an alternative source of LCs. Methods: To develop a safer, simple, and rapid strategy to generate human LC-like cells (LLCs) from stem cells, we first performed preliminary tests under different conditions for the induction of LLCs from human CD34/CD73 double positive-testis-derived stem cells (HTSCs). Based on the embryological sequence of events, we suggested a 3-step strategy for the differentiation of human ESCs into LLCs. We generated the mesendoderm in the first stage and intermediate mesoderm (IM) in the second stage and optimized the conditions for differentiation of IM into LLCs by comparing the secreted testosterone levels of each group. Results: HTSCs and human embryonic stem cells can be directly differentiated into LLCs by defined molecular compounds within a short period. Human ESC-derived LLCs can secrete testosterone and express steroidogenic markers. Conclusion: We developed a rapid and efficient protocol for the production of LLCs from stem cells using defined molecular compounds. These findings provide a new therapeutic cell source for male hypogonadism.

Nanofiber-Based in Vitro System for High Myogenic Differentiation of Human Embryonic Stem Cells

2013 ◽  
Vol 14 (12) ◽  
pp. 4207-4216 ◽  
Author(s):  
Matthew Leung ◽  
Ashleigh Cooper ◽  
Soumen Jana ◽  
Ching-Ting Tsao ◽  
Timothy A. Petrie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Myogenic Differentiation ◽  
Embryonic Stem ◽  
In Vitro System

Smooth-Muscle-Like Cells Derived from Human Embryonic Stem Cells Support and Augment Cord-Like Structures In Vitro

2010 ◽  
Vol 6 (2) ◽  
pp. 237-247 ◽  
Author(s):  
Elaine Vo ◽  
Donny Hanjaya-Putra ◽  
Yuanting Zha ◽  
Sravanti Kusuma ◽  
Sharon Gerecht
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem
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