Recurrent outbreaks of infectious bursal disease (IBD) in a layer farm caused by very virulent IBD virus (vvIBDV) in India: Pathology and molecular analysis

2013 ◽  
Vol 3 (4) ◽  
pp. 200-206
Author(s):  
R. Barathidasan ◽  
S. D. Singh ◽  
M. Asok Kumar ◽  
P. A. Desingu ◽  
M. Palanivelu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetic Analysis ◽  
Uttar Pradesh ◽  
Infectious Bursal Disease ◽  
Nucleotide Sequencing ◽  
Rt Pcr ◽  
Vp2 Gene ◽  
Amino Acid Homology ◽  
Bursal Disease ◽  
Layer Farm

The present study was carried out to investigate and characterize the nature of infectious bursal disease virus (IBDV) involved in the recurrent outbreaks in an experimental layer farm in Bareilly, Uttar Pradesh, India using direct tissue reverse transcription- polymerase chain reaction (RT-PCR) followed by nucleotide sequencing of VP2 gene. A total of three acute IBD outbreaks with the interval of 4-6 months were recorded in the batch of 3-5 weeks-old layer chicks in the year 2012-2013. Tissues collected from necropsied birds were found RT-PCR positive for IBDV VP2 gene. Genetic analysis of the sequenced VP2 gene revealed the IBDV belonged to very virulent (vv) subtype and had amino acids at positions 222A, 256I, 294I, and 299S typical for vvIBDV strains isolated worldwide. It had only one unique amino acid change in the antigenic peak A (210-225 aa) at position 212DàN (AspàAsn), which is not observed in any of the vvIBDVs isolated in India and abroad. Phylogenetic analysis revealed the isolates were more closely related to vvIBDV strains rA and rB (U.S.A.), Gx and HLJ-7 (China), OKYM (Japan), and shared >95% nucleotide homology with them. The VP2 gene shared 96.7% amino acid homology with IBDI+ vaccine strain used in India, comparatively higher among other vaccines strains, suggesting that IBD intermediate plus (IBDI+) vaccine might provide optimum cross protection, also for other vvIBDV strains. The vvIBDV strains remain a threat to poultry industry worldwide, and require regular monitoring and genetic analysis in order to keep track of the appearance and evolution of antigenically different IBDV strains or subtypes.    

scholarly journals Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey

2012 ◽  
Vol 48 (No. 12) ◽  
pp. 359-362 ◽  
Author(s):  
G. Ozbes ◽  
Ertas HB ◽  
A. Muzo
Keyword(s):  
Rflp Analysis ◽  
Infectious Bursal Disease ◽  
Polymorphism Analysis ◽  
Rt Pcr ◽  
Vp2 Gene ◽  
Infectious Bursal Disease Viruses ◽  
Pcr Rflp ◽  
Bursal Disease ◽  
Polymerase Chain ◽  
Rflp Assay

Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay. The assay amplifies a 743 bp fragment of the IBDV VP2 gene. The RFLP profiles of 40 of these positive samples were determined using the enzyme MboI. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity exists among IBDV strains in a infected flock.

scholarly journals Identification of infectious bursal disease virus with atypical VP2 amino acid profile in Latvia

2017 ◽  
Vol 61 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Anna Pikuła ◽  
Katarzyna Domańska-Blicharz ◽  
Rytis Cepulis ◽  
Krzysztof Śmietanka
Keyword(s):  
Amino Acid ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Genetic Relatedness ◽  
Amino Acid Profile ◽  
Infectious Bursal Disease ◽  
Poultry Industry ◽  
Vp2 Gene ◽  
Detection And Identification ◽  
Bursal Disease

AbstractIntroduction: Infectious bursal disease virus (IBDV) is a causative agent of immunosuppressive disorder resulting in significant losses to the world poultry industry. This study describes the molecular characterisation of an atypical IBDV from a field outbreak that occurred in vaccinated chicken flocks in Latvia in 2011.Material and Methods: Ten bursae of Fabricius from each flock were collected for laboratory examination. Virus isolation was performed in embryonated eggs and CEF culture. The RT-PCR aimed at hypervariable domain of VP2 gene combined with sequencing was performed for detection and identification of IBDV.Results: The molecular examinations confirmed the IBDV infection. The analysis of the amino acid sequence revealed that the strain possessed four amino acids at VP2 protein (222A, 256I, 294I, and 299S), indicating a genetic relatedness to a very virulent IBDV. However, some unique or rare amino acid substitutions (219L, 220F, 254D, 279N, and 280T) were also detected.Conclusion: The obtained results demonstrate the occurrence of IBDV with a high mutation rate within the hypervariable domain of VP2 peptide, and highlight the necessity of implementation of IBDV surveillance in Eastern European poultry industry to determine whether this strain is an exception or a new wave of IBDV with new genetic features emerged in the field.

Molecular and Cultures based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Vaccinated and Non Vaccinated Poultry Flocks 

2021 ◽  
Author(s):  
Ritesh S. Shinde ◽  
Harshad C. Chauhan ◽  
Sandip S. Patel ◽  
Kishan Kumar Sharma ◽  
Arun C. Patel ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Infectious Bursal Disease ◽  
Fibroblast Cells ◽  
Culture Result ◽  
Rt Pcr ◽  
Culture Methods ◽  
Bursal Disease ◽  
History Of ◽  
Chicken Fibroblast ◽  
Pooled Sample

Background: In recent years, Infectious bursal disease is continuously occurring even after vaccination in India and requires an inclusive diagnosis. Therefore, the present study was undertaken to diagnose IBD through molecular and culture methods. Methods: One pooled sample, from each of 54 flocks having birds with IBD like symptoms, was collected. History of bird type, age and vaccination was recorded. Samples were subjected to RT-PCR, egg embryo culture and chicken fibroblast cells culture. Result: A total of 49669 out of 517900 (9.59 %) of birds, aging 3-6 weeks, were displaying the signs similar to IBD.In RT-PCR, 21 (38.88%) samples were found positive which belonged to11 (52.38%) vaccinated and 10 (47.62%) unvaccinated flocks.The RT-PCR positive samples were successfully cultivated for the virus through egg embryo and cell culture. The CEF culture was found least sensitive compared to egg embryo culture and RT-PCR. 

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