scholarly journals Characterizing nanoscale changes in the activity of VEGFR-2 on glioma microvascular endothelial cell membranes using atomic force microscopy

2016 ◽  
Vol 13 (2) ◽  
pp. 483-488 ◽  
Author(s):  
Dexiang Zhou ◽  
Shengquan Zhan ◽  
Dong Zhou ◽  
Peng Wang ◽  
Guangzhong Chen ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cell ◽  
Cell Membranes ◽  
Microvascular Endothelial Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microvascular Endothelial

scholarly journals Atomic Force Microscopy of the Structure of Red Blood Cell Membranes in Acute Blood Loss and Reinfusion

2009 ◽  
Vol 5 (5) ◽  
pp. 5
Author(s):  
V. V. Moroz ◽  
A. M. Chernysh ◽  
Ye. K. Kozlova ◽  
A. K. Kirsanova ◽  
I. S, Novoderzhkina ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Loss ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Acute Blood Loss ◽  
Force Microscopy ◽  
Atomic Force ◽  
Red Blood Cell Membranes

scholarly journals Stiffness and heterogeneity of the pulmonary endothelial glycocalyx measured by atomic force microscopy

2011 ◽  
Vol 301 (3) ◽  
pp. L353-L360 ◽  
Author(s):  
Ryan O'Callaghan ◽  
Kathleen M. Job ◽  
Randal O. Dull ◽  
Vladimir Hlady
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Indentation Depth ◽  
Cell Junctions ◽  
Endothelial Glycocalyx ◽  
Cell Junction ◽  
Microvascular Endothelial Cell ◽  
Cell Contact ◽  
Force Microscopy ◽  
Atomic Force

The mechanical properties of endothelial glycocalyx were studied using atomic force microscopy with a silica bead (diameter ∼18 μm) serving as an indenter. Even at indentations of several hundred nanometers, the bead exerted very low compressive pressures on the bovine lung microvascular endothelial cell (BLMVEC) glycocalyx and allowed for an averaging of stiffness in the bead-cell contact area. The elastic modulus of BLMVEC glycocalyx was determined as a pointwise function of the indentation depth before and after enzymatic degradation of specific glycocalyx components. The modulus-indentation depth profiles showed the cells becoming progressively stiffer with increased indentation. Three different enzymes were used: heparinases III and I and hyaluronidase. The main effects of heparinase III and hyaluronidase enzymes were that the elastic modulus in the cell junction regions increased more rapidly with the indentation than in BLMVEC controls, and that the effective thickness of glycocalyx was reduced. Cytochalasin D abolished the modulus increase with the indentation. The confocal profiling of heparan sulfate and hyaluronan with atomic force microscopy indentation data demonstrated marked heterogeneity of the glycocalyx composition between cell junctions and nuclear regions.

scholarly journals The Effect of the Endothelial Cell Cortex on Atomic Force Microscopy Measurements

2013 ◽  
Vol 105 (2) ◽  
pp. 300-309 ◽  
Author(s):  
R. Vargas-Pinto ◽  
H. Gong ◽  
A. Vahabikashi ◽  
M. Johnson
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cell ◽  
Cell Cortex ◽  
Force Microscopy ◽  
Atomic Force

"Holes" on Red Blood Cell Membranes Including Thalassemia Imaged by Atomic Force Microscopy

2004 ◽  
Vol 10 (S02) ◽  
pp. 1110-1111
Author(s):  
TAPAN Guha ◽  
RADHABALLAV Bhar ◽  
KAJAL Bhattacharyya ◽  
RATANLAL L Brahmachary
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Red Blood Cell Membranes

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

scholarly journals Adhesively-Tensed Cell Membranes: Lysis Kinetics and Atomic Force Microscopy Probing

2003 ◽  
Vol 85 (4) ◽  
pp. 2746-2759 ◽  
Author(s):  
Alina Hategan ◽  
Richard Law ◽  
Samuel Kahn ◽  
Dennis E. Discher
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membranes ◽  
Force Microscopy ◽  
Atomic Force
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