scholarly journals Atomic Force Microscopy of the Structure of Red Blood Cell Membranes in Acute Blood Loss and Reinfusion

2009 ◽  
Vol 5 (5) ◽  
pp. 5
Author(s):  
V. V. Moroz ◽  
A. M. Chernysh ◽  
Ye. K. Kozlova ◽  
A. K. Kirsanova ◽  
I. S, Novoderzhkina ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Loss ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Acute Blood Loss ◽  
Force Microscopy ◽  
Atomic Force ◽  
Red Blood Cell Membranes

"Holes" on Red Blood Cell Membranes Including Thalassemia Imaged by Atomic Force Microscopy

2004 ◽  
Vol 10 (S02) ◽  
pp. 1110-1111
Author(s):  
TAPAN Guha ◽  
RADHABALLAV Bhar ◽  
KAJAL Bhattacharyya ◽  
RATANLAL L Brahmachary
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Red Blood Cell Membranes

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

scholarly journals Impairments in the Nanostructure of Red Blood Cell Membranes in Acute Blood Loss and Their Correction with Perfluorocarbon Emulsion

2011 ◽  
Vol 7 (2) ◽  
pp. 5 ◽  
Author(s):  
V. V. Moroz ◽  
A. M. Chernysh ◽  
E. K. Kozlova ◽  
V. A. Sergunova ◽  
O. E, Gudkova ◽  
...  
Keyword(s):  
Blood Loss ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Acute Blood Loss ◽  
Perfluorocarbon Emulsion ◽  
Red Blood Cell Membranes

scholarly journals Changes in the Surface of Red Blood Cell Membranes after Blood Loss and Their Correction with Laser Irradiation

2010 ◽  
Vol 6 (2) ◽  
pp. 5 ◽  
Author(s):  
V. V. Moroz ◽  
A. K. Kirsanova ◽  
I. S. Novoderzhkina ◽  
Ye. K., Kozlova ◽  
P. Yu. Borshchegovskaya ◽  
...  
Keyword(s):  
Blood Loss ◽  
Blood Cell ◽  
Laser Irradiation ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Red Blood Cell Membranes

93. Influnce of glycerol and cryopreservation on red blood cell shape and band 3 conformation studied by atomic force microscopy

Cryobiology ◽  
2006 ◽  
Vol 53 (3) ◽  
pp. 407
Author(s):  
Olga A. Kofanova ◽  
Michael R. Mueller ◽  
Ingolf Bernhardt
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Shape ◽  
Band 3 ◽  
Force Microscopy ◽  
Atomic Force

STRUCTURE OF INTRACRANIAL VEINS AND ELASTIC-VISCOUS PROPERTIES OF RED BLOOD CELL MEMBRANES IN ADOLESCENTS WITH CONNECTIVE TISSUE DYSPLASIA

2021 ◽  
pp. 47-60
Author(s):  
A.N. Proshin ◽  
V.V. Mashin ◽  
L.A. Belova ◽  
D.V. Belov
Keyword(s):  
Connective Tissue ◽  
Blood Cell ◽  
Young’S Modulus ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Young's Modulus ◽  
Main Group ◽  
Atomic Force ◽  
Connective Tissue Dysplasia ◽  
Red Blood Cell Membranes

The aim of the study is to examine the characteristics of intracranial veins (magnetic resonance angiography), and the elastic-viscous properties of the red blood cell membranes (atomic force microscopy) in adolescents with connective tissue dysplasia. Material and Methods. The authors examined 156 adolescents. The main group consisted of 82 adolescents with connective tissue dysplasia. The comparison group included 74 adolescents without connective tissue dysplasia. Connective tissue dysplasia was assessed according to L.N. Abakumova scale (2006). Magnetic resonance angiography was performed on a Siemens Magnetom Symphony apparatus equipped with a superconducting magnetic system with 1.5 Tesla field strength. Preparations of dry red blood cells were made to study red blood cell cytoplasmic membrane. The specimen was scanned using an atomic force microscope SOLVER P47-Pro. Results. The majority of adolescents from the main group demonstrated anatomical variants of the dura mater sinuses structure. At the same time, sinus hypoplasia in patients of the main group was diagnosed more often than asymmetry of these structures. In the comparison group, the number of anatomical variants of the dura mater sinuses structure also prevailed over the symmetrical structure. However, the sinuses asymmetry was more often. Comparative analysis of Young's modulus values in two groups of patients showed a statistically significant superiority of this indicator in the main group. Correlation analysis of the connective tissue dysplasia severity and Young's modulus values revealed a moderate direct correlation. Conclusion. Thus, it can be assumed that in patients with connective tissue dysplasia the cerebral vessel structure and elastic properties of red blood cell membranes formed during embryogenesis impair the functional and adaptive capabilities of intracranial vessels, contributing to the development and progression of cerebral venous disorders and their subsequent clinical manifestation. Key words: intracranial veins, Young's modulus, connective tissue dysplasia. Цель. Исследовать при помощи магнитно-резонансной ангиографии особенности интракраниальных вен и методом атомно-силовой микроскопии – упруго-вязкостные свойства мембран эритроцитов подростков с соединительнотканной дисплазией. Материалы и методы. Обследовано 156 подростков. Основную группу (ОГ) составили 82 подростка с признаками дисплазии соединительной ткани (ДСТ), группу сравнения (ГС) – 74 подростка без проявлений ДСТ. ДСТ оценивали по шкале Л.Н. Абакумовой (2006). Магнитно-резонансная ангиография проводилась на аппарате Siemens Magnetom Symphony, оснащенном сверхпроводящей магнитной системой с силой поля 1,5 Тесла. С целью изучения состояния цитоплазматической мембраны эритроцитов были изготовлены сухие препараты эритроцитов. Препарат подвергался сканированию, которое проводилось с помощью атомно-силового микроскопа SOLVER P47-Pro. Результаты. У большинства подростков ОГ выявлены анатомические варианты строения синусов твердой мозговой оболочки. При этом гипоплазия синусов у пациентов ОГ диагностировалась чаще, чем асимметрия данных структур. В ГС количество анатомических вариантов строения синусов твердой мозговой оболочки также превалировало над симметричным строением, но чаще диагностировалась асимметрия синусов. Сравнительный анализ значений модуля Юнга в двух группах пациентов показал статистически значимое превосходство данного показателя в ОГ. Корреляционный анализ степени выраженности ДСТ и значений модуля Юнга выявил умеренную прямую корреляцию. Выводы. Можно предположить, что имеющиеся при ДСТ особенности структуры церебральных сосудов и эластических свойств мембран эритроцитов, заложенные в процессе эмбриогенеза, ухудшают функциональные и адаптационные возможности интракраниальных сосудов, что способствует возникновению и прогрессированию церебральных венозных расстройств и их последующей клинической манифестации. Ключевые слова: интракраниальные вены, модуль Юнга, дисплазия соединительной ткани.

Changes in red blood cell membrane structure in G6PD deficiency: An atomic force microscopy study

2015 ◽  
Vol 444 ◽  
pp. 264-270 ◽  
Author(s):  
Jia Tang ◽  
Chengrui Jiang ◽  
Xiao Xiao ◽  
Zishui Fang ◽  
Lei Li ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
G6pd Deficiency ◽  
Membrane Structure ◽  
Microscopy Study ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Measurement of the Local Tension of Red Blood Cell Membranes by Atomic Force Spectroscopy

2013 ◽  
Vol 9 (1) ◽  
pp. 14 ◽  
Author(s):  
V. A. Sergunova ◽  
O. E. Gudkova ◽  
A. P. Kozlov ◽  
A. M. Chernysh
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Force Spectroscopy ◽  
Atomic Force Spectroscopy ◽  
Atomic Force ◽  
Red Blood Cell Membranes

scholarly journals Human Red Blood Cell Adhesion to Laminin Measured by Atomic Force Microscopy

2015 ◽  
Vol 108 (2) ◽  
pp. 167a ◽  
Author(s):  
George Lykotrafitis
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Adhesion ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Force Microscopy ◽  
Atomic Force

Ultrastructural changes in red blood cells in aortic dissection patients under extracorporeal circulation: Atomic force microscopy study

2018 ◽  
Vol 41 (8) ◽  
pp. 452-459
Author(s):  
Li Deng ◽  
Hui Yang ◽  
Weixin Meng ◽  
Yue Chen ◽  
Guang Hu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Aortic Dissection ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Extracorporeal Circulation ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Scanning Electron

Purpose: During extracorporeal circulation in heart surgery, blood is exposed to non-physiological conditions, such as high shear stress, foreign surfaces, turbulence, and hypothermia. These factors cause damage to the red blood cells, which is manifested by immediate and delayed hemolysis or some changes in the mechanical properties of red blood cells, defined as sublethal trauma. Unfortunately, sublethal trauma is hard to detect, and there is not enough morphological evidence regarding red blood cell sublethal trauma. In this study, red blood cell sublethal trauma was observed after extracorporeal circulation by describing ultrastructural changes in red blood cell membranes using atomic force microscopy and scanning electron microscopy. Methods: Venous blood (2 mL) was collected into heparin tubes from preoperative, intraoperative and postoperative aortic dissection patients for comparison with blood from healthy patients. The red blood cell morphological study (malformations percentage, diameter, height, concavity, and roughness) was performed with scanning electron microscopy and atomic force microscopy. Results: Scanning electron microscopy and atomic force microscopy imaging analysis revealed that the red blood cell shape changed during extracorporeal circulation and that the red blood cell malformation percentage in the postoperative group was higher than those in the preoperative and intraoperative groups. Most morphological parameters had no obvious changes, except roughness (Ra and Rq) in aortic dissection patients. Atomic force microscopy quantitative analysis indicated that the roughness of red blood cell membranes increased during extracorporeal circulation. Conclusions: This study demonstrates that ultrastructural morphological damage occurs to red blood cells membranes due to extracorporeal circulation in aortic dissection patients. In addition, we provided a new parameter (Ra and Rq) to evaluate red blood cell sublethal trauma.

Atomic force microscopy study of red blood cell membrane nanostructure during oxidation-reduction processes

2018 ◽  
Vol 31 (10) ◽  
pp. e2724 ◽  
Author(s):  
E. Kozlova ◽  
A. Chernysh ◽  
V. Sergunova ◽  
O. Gudkova ◽  
E. Manchenko ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Microscopy Study ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Reduction Processes ◽  
Atomic Force ◽  
Oxidation Reduction
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