scholarly journals Differential expression of PTEN in hepatic tissue and hepatic stellate cells during rat liver fibrosis and its reversal

2012 ◽  
Vol 30 (6) ◽  
pp. 1424-1430 ◽  
Author(s):  
LIBO ZHENG ◽  
XIULI CHEN ◽  
JINBO GUO ◽  
HUICONG SUN ◽  
LEI LIU ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Differential Expression ◽  
Rat Liver ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Hepatic Tissue ◽  
Rat Liver Fibrosis

scholarly journals A Novel Matrine Derivative WM130 Inhibits Activation of Hepatic Stellate Cells and Attenuates Dimethylnitrosamine-Induced Liver Fibrosis in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Yang Xu ◽  
Zhangxiao Peng ◽  
Weidan Ji ◽  
Xiang Li ◽  
Xuejing Lin ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Rat Liver ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Critical Event ◽  
Pharmacological Activities ◽  
Fibrosis Progression ◽  
Collagen Iii ◽  
Rat Liver Fibrosis ◽  
Low Toxicity

Activation of hepatic stellate cells (HSCs) is a critical event in process of hepatic fibrogenesis and cirrhosis. Matrine, the active ingredient ofSophora, had been used for clinical treatment of acute/chronic liver disease. However, its potency was low. We prepared a high potency and low toxicity matrine derivate, WM130 (C30N4H40SO5F), which exhibited better pharmacological activities on antihepatic fibrosis. This study demonstrated that WM130 results in a decreased proliferative activity of HSC-T6 cells, with the half inhibitory concentration (IC50) of 68 μM. WM130 can inhibit the migration and induce apoptosis in HSC-T6 cells at both concentrations of 68 μM (IC50) and 34 μM (half IC50). The expression ofα-SMA, Collagen I, Collagen III, and TGF-β1 could be downregulated, and the protein phosphorylation levels of EGFR, AKT, ERK, Smad, and Raf (p-EGFR, p-AKT, p-ERK, p-Smad, and p-Raf) were also decreased by WM130. On the DMN-induced rat liver fibrosis model, WM130 can effectively reduce the TGF-β1, AKT,α-SMA, and p-ERK levels, decrease the extracellular matrix (ECM) formation, and inhibit rat liver fibrosis progression. In conclusion, this study demonstrated that WM130 can significantly inhibit the activation of HSC-T6 cells and block the rat liver fibrosis progression by inducing apoptosis, suppressing the deposition of ECM, and inhibiting TGF-β/Smad and Ras/ERK pathways.

The anti-fibrotic effect of Aramchol on rat liver fibrosis induced by TAA and in vitro on primary hepatic stellate cells

2018 ◽  
Vol 68 ◽  
pp. S407
Author(s):  
R. Golan-Gerstl ◽  
M. Valitsky ◽  
R. Oren ◽  
E. Brazowski ◽  
L. Hayardeni ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Rat Liver ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Rat Liver Fibrosis
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